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51.
Y Zhou V Moennig C O Coulibaly J Dahle B Liess 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(1):76-80
Monoclonal antibodies against hog cholera and bovine viral diarrhoea viruses were assayed on organ tissue sections of experimentally infected animals. The animals had been infected simultaneously with both viruses. The antibodies were tested using an indirect immunofluorescence test and an indirect enzyme immunoassay with a biotin/streptavidin/peroxidase detection system. A polyclonal hyperimmune serum was used as a control in direct immunofluorescence tests. Both techniques based on monoclonal antibodies were more sensitive and more specific than the conventional test, the enzyme immunoassay being more sensitive than the immunofluorescence test. Small amounts of BVD viral antigen were demonstrable with monoclonal antibodies in most organ tissues. 相似文献
52.
T J Weckman C L Tai W E Woods H H Tai J W Blake T Tobin 《American journal of veterinary research》1989,50(4):502-507
Pharmacologic effects of alpha-methylfentanyl and 3-methylfentanyl, analogs of fentanyl, were investigated in mares. The ability of an 125I-labeled fentanyl radioimmunoassay (125I-RIA) to detect these methylated fentanyl analogs in individual and pooled urine samples from horses was evaluated. Also, the ability of 7 fentanyl antibodies to react with fentanyl and fentanyl derivatives (sufentanil, alfentanil, and carfentanil) was investigated. Mares were studied in a locomotor test to determine the amount of stimulation methylated fentanyl analogs might induce. Two mares each were given alpha-methylfentanyl at 1, 2, 4, 8, or 13 micrograms/kg of body weight, IV, or 3-methylfentanyl at 0.4, 0.7, or 1 microgram/kg IV. The cross-reactivity of sufentanil, alfentanil, carfentanil, alpha-methylfentanyl, and 3-methylfentanyl with 7 fentanyl antibodies was studied, using the 125I-RIA. All fentanyl analogs, with the exception of alfentanil, cross-reacted well with a C1 antibody raised to fentanyl. Less satisfactory cross-reactivity was determined with 6 other antibodies raised to fentanyl derivatives. When the C1 antibody was combined with an iodinated analog to fentanyl, good detectability of alpha-methylfentanyl and 3-methylfentanyl, in terms of fentanyl equivalents, was obtained from urine samples of dosed mares. The ability of the 125I-RIA to detect methylated fentanyl analogs in forensic urine samples pooled in groups of up to 20 samples was evaluated. When these methylated analogs were administered to mares in doses that induced measurable locomotor stimulation, the analog's presence was readily detected in individual or pooled samples. 相似文献
53.
M C Rick 《Veterinary Clinics of North America: Equine Practice》1989,5(2):407-428
Diagnosis, evaluation, and management of the various grades of rectal tears is discussed. Surgical techniques, which include direct closure, diverting colostomies, and placement of temporary rectal liners, are detailed. Also, rectal prolapses and various methods of repair are outlined. 相似文献
54.
Enzyme-linked immunosorbent assay for the detection of circulating antigens of Toxoplasma gondii in the serum of cats 总被引:1,自引:0,他引:1
M R Lappin C E Greene A K Prestwood D L Dawe R L Tarleton 《American journal of veterinary research》1989,50(9):1586-1590
An ELISA was developed to detect circulating antigens of Toxoplasma gondii in the serum of cats. For the experiment, toxoplasmosis was induced in a group of cats by oral administration of bradyzoites. An ELISA that detects anti-Toxoplasma IgG, an ELISA to detect circulating antigens, and fecal examinations were performed on samples from each cat for 1 year after inoculation. When coupled with IgG-class antibody measurement, antigen detection can aid in the diagnosis of some cases of subclinical feline toxoplasmosis. 相似文献
55.
A B Gardner C E Gardner 《Veterinary Clinics of North America: Food Animal Practice》1989,5(3):653-664
Dairy practice often requires substantial time commitments. Those veterinarians with families may find that if they do not actively budget some time for spouse and children, the family unit may suffer. A successful practice and meaningful family life are both possible if the doctor or doctors involved are willing to work for their achievement. 相似文献
56.
M. A. Gomez J. A. Navarro S. Gomez P. Camara J. C. Gomez A. Bernabé 《Anatomia, histologia, embryologia》1989,18(4):305-315
The structure and ultrastructure of the adenohypophyseal pars distalis in kids of the Murciano-granadiana breed (18 males and 12 females), aged 2-3 months and with an average carcass weight of 8 kg has been studied. Techniques of staining (Tetrachrome Herlant's, and Cleveland-Wolfe) and histochemistry (PAS, PAS-OG and BA-PAS-OG) contrasted with immunolabelling (PAP) have been used. In addition an ultrastructural study has been made and nucleus and cytoplasm, secretory granules, mitochondria and lysosomes have been measured with a semiautomatic image analyzer (IBAS-1). TSH cells are found in sagittal section in the anterior area and in an antero-caudodorsal band, and transversally in the ventral and medial region; they are 6% and their average size is 14.15 microns. Ultrastructurally they are a single cellular type with spherical granules whose size is 195 nm in males and 149 in females; these granules are characterized by a clear halo and a variable electronic density. The rough endoplasmic reticulum is found as slightly enlarged vesicles with a homogeneous and moderately electro-dense content or in concentric stratifications. 相似文献
57.
Three strip test kits which measure calcium and/or magnesium in mares' mammary secretions were compared and assessed for their accuracy in:- 1) measuring these electrolytes compared to standard laboratory measurements, and 2) predicting the time of parturition. Daily samples of mammary secretions were obtained pre-partum from 35 Thoroughbred mares and allocated a score. Measurement of mammary secretion calcium and/or magnesium by the strip tests and laboratory methods correlated significantly (P less than 0.001). The results obtained for prediction of parturition were analysed by two methods. Method i) indicated that the majority of mares had a maximum score on the day of birth. However, the second method of analysis included all prepartum scores and this demonstrated that maximum scores occurred on more occasions prior to the day of birth than on the day of birth itself. The number of nights spent attending mares, and the percentage of occasions when foaling was missed, were also calculated for given strip test scores. The results indicate that the test kits are not particularly accurate in predicting time of parturition although they are a reliable means of measuring mammary secretion calcium and/or magnesium. It is suggested that the test kits may be more helpful by indicating when it is not necessary to attend mares at night. 相似文献
58.
The ultrastructure of sarcocysts of macro- and microscopic species of Sarcocystis was compared from naturally infected water buffalo from India. Grossly visible sarcocysts had walls consisting of cauliflower-like villar protrusions, typical of S. fusiformis. The sarcocyst wall of the microscopic species of Sarcocystis was 6.4 microns thick and consisted of tightly packed conical villar protrusions that were 9.6 microns long and 3.7 microns wide at the base. At approximately 3 microns above the base, the distal two-thirds of the villar protrusion became conical shaped and was bent laterally at an angle of 45 degrees to the sarcocyst surface. The granular layer beneath the villar protrusions was 0.9 microns thick. In S. levinei the granular layer was 1.9 microns thick, the villar protrusions were narrow and it had a highly undulating primary cyst wall. Whether the microscopic S. levinei-like sarcocysts of Indian and Malaysian water buffalo are distinct species of Sarcocystis will require further investigation. 相似文献
59.
Effect of pelleting and beta-glucanase supplementation on the ileal and fecal digestibility of a barley-based diet in the pig 总被引:5,自引:0,他引:5
The influences of pelleting and beta-glucanase supplementation on the digestibility of dietary components in a barley-based diet were investigated in pigs fitted with cannulas in the terminal ileum. The diet contained 49.0% starch, 18.9% crude protein and 14.8% dietary fiber, with arabinoxylans (4.9%), mixed-linked beta-glucans (3.4%) and cellulose (3.3%) as the main fiber components. Pelleting increased mixed-linked beta-glucan solubility from 45 to 62%. Neither treatment influenced the ileal or fecal apparent digestibilities of dry matter (64 and 80%, respectively), energy (62 and 79%), crude protein (53 and 75%), crude fat (26 and 27%) or dietary fiber (58 and 72%). However, pelleting increased (P less than .01) the pre-ileal apparent digestibility of starch from 91.5 to 95.3% and decreased (P less than .03) that of ash from -10 to -23%, while increasing (P less than .005) the fecal apparent digestibility of starch from 98.6 to 99.7%. The solubility of mixed-linked beta-glucans in ileal digesta was lower (P less than .001) in pelleted (26%) than in unpelleted (58%) diets. beta-glucanase supplementation also increased (P less than .05) the ileal apparent digestibility of starch, from 92.6 to 94.3%, and of mixed-linked beta-glucans, from 95.7 to 97.1%. Significant between-pig differences in the apparent digestibility of dietary components were observed. In conclusion, treatments that disrupt the endosperm cell walls in barley can increase the proportion of the diet digested prior to the large intestine. 相似文献
60.
C M Honnas 《Veterinary Clinics of North America: Equine Practice》1991,7(3):695-722
Several surgical conditions affecting the foot can be successfully managed with the horse standing. Many factors affect the clinician's choice of whether to perform surgery with the horse standing or anesthetized. Temperament or pregnancy of the horse and economics may influence the decision to perform surgery with the horse standing. This article discusses several foot conditions that can be treated successfully in the ambulatory horse. 相似文献