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41.
C Dorn A Schroeter A Miko D Protz R Helmuth 《Berliner und Münchener tier?rztliche Wochenschrift》2001,114(5-6):179-183
In the last years the number of isolations of Salmonella enterica subspecies enterica serovar paratyphi B (S. paratyphi B) sent to the national salmonella reference laboratory of Germany has increased steadily. Most of the isolates originated from fowl or poultry products. The bacteriological, serological and biochemical properties of the isolates were investigated. Special emphasis was given to the utilization of d-tartrate which subgroups the serovar. All of them belonged to the d-tartrate positive variant, which is generally considered less virulent for humans and was formerly called S. java. The performance of various tests is compared and in addition the possibility of the spread within the production line is discussed. 相似文献
42.
A Yabuki S Suzuki M Matsumot M Kurohmaru Y Hayashi H Nishinakagawa 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(12):1339-1342
The proximal straight tubules of the female mouse kidney exhibit heavy periodic acid Schiff (PAS) staining in their brush borders and numerous cytoplasmic granules. In the present study, the female DBA/2Cr mouse kidney was examined, using various fixatives (formalin, PFA, PLP, Zamboni's, Bouin, or Carnoy solution) and various staining methods (HE, PAS, alcian blue, periodic acid methenamine-silver (PAM), toluidine blue, azan, or Congo red). Under azan and PAM, the staining pattern of the brush border was similar to that of PAS, and few effects of the fixative were observed. Cytoplasmic granules were clearly detected with PAM as well as PAS. However, these granules were not detected with Carnoy solution. Furthermore, distribution of granules differed between PAS and PAM. 相似文献
43.
Pilot study of the effect of acemannan in cats infected with feline immunodeficiency virus. 总被引:7,自引:0,他引:7
K M Yates L J Rosenberg C K Harris D C Bronstad G K King G A Biehle B Walker C R Ford J E Hall I R Tizard 《Veterinary immunology and immunopathology》1992,35(1-2):177-189
Acemannan, a complex carbohydrate shown to stimulate interleukin-1, tumor necrosis factor alpha and prostaglandin E2 production by macrophages, has also demonstrated antiviral activity in vitro against human immunodeficiency virus, Newcastle disease virus and influenza virus. A pilot study was undertaken to determine acemannan's effect in 49 feline immunodeficiency virus (FIV) infected cats with clinical signs of disease (Stage 3, 4 or 5), 23 of which had severe lymphopenia. Cats received acemannan either by intravenous (Group 1) or subcutaneous (Group 2) injection once weekly for 12 weeks, or by daily oral (Group 3) administration for 12 weeks. Upon entry into the study, cats were randomly assigned to one of the three groups. Laboratory analyses were performed at the beginning of the study and at Weeks 6 and 12. Cats were allowed to continue with a predetermined maintenance regimen of acemannan after completing the 12-week study. Thirteen cats died during the course of treatment. Upon necropsy, the most frequent histopathologic findings were neoplastic, kidney and pancreatic disease. Friedman's two-way ANOVA test showed no significant differences in efficacy among groups administered acemannan by the different routes. Therefore, groups were combined and a signed-ranks test was used to determine changes over time. A significant increase was seen in lymphocyte counts (P < 0.001). Neutrophil counts decreased significantly (P = 0.007), as did incidence of sepsis (P = 0.008). When cats entering with lymphopenia were analyzed separately, a much greater increase in lymphocyte counts was noted (235%) compared with non-lymphopenic cats (42%). A survival rate of 75% was found for all three groups. Thirty-six of 49 animals are alive 5-19 months post-entry. These results suggest that acemannan therapy may be of significant benefit in FIV-infected cats exhibiting clinical signs of disease. 相似文献
44.
45.
Abstract. A humoral antibody response was demonstrated by ELISA in rainbow trout immunized intraperitoneally with extracts from the branchiuran ectoparasite Argulus foliaceus. A similar immunization protocol produced a higher titre response in a rabbit. Both trout and rabbit identified several antigenic components on immunoblots. ELISA and immunoblotting indicated that rainbow trout and rabbit anti-A. foliaceus sera identified components from the parasitic copepods Lepeophtheirus salmonis and Caligus elongatus. 相似文献
46.
47.
Behaviour of laying hens in a deep litter house 总被引:4,自引:0,他引:4
1. Flocks of medium hybrid laying hens were housed in a modified deep litter system; the house was divided into 2, 3 and 4 pens in three successive years. Flock size was 300 or 370 and stocking density varied from 3.4 to 10.7 birds/m2. Higher densities used were above those recommended by the Ministry of Agriculture, Fisheries and Food (MAFF). 2. A random sample of 100 birds was identified with individual tags in each of the 9 flocks; regular observations using a scanning technique were made in each laying cycle to determine bird location and behaviour. 3. In all flocks the use of area by some individuals was uneven, that is, they were sighted in certain areas significantly more often than would have been expected by chance. The proportion of such individuals varied between flocks from 35 to 65%. Overall, birds spent more time on the slatted area than would have been expected from the area that it occupied. 4. A wide variety of different behaviour patterns was observed both on litter and on slats, but with foraging occurring more on litter and feeding more on slats. 5. Movement appeared to be constrained by crowding, because time spent in locomotion decreased in approximately linear fashion with increased stocking density. This provides support for MAFF recommendations of limits on stocking density in deep litter houses. 相似文献
48.
在查清天津市园林主要花灌木刺吸口器害虫桃粉蚜、绣线菊蚜、棉蚜和山楂红蜘蛛发生消长规律的基础上 ,确定了防治适期和结合点 ,筛选出 10 %吡虫啉、绿灵、蚧螨灵、 1. 8%齐螨素和花保5种低、无毒药剂 ,防效均达到 90 %以上。查出天敌 13种 ,并对优势种作了取食量调查 相似文献
49.
根据饲料可消化氨基酸研制肉仔鸡无鱼粉饲粮试验 总被引:1,自引:0,他引:1
选用240只AA商品代出壳肉仔鸡进行试验,以探讨根据饲料中可消化氨基酸含量,研制菜籽粕型无鱼粉低蛋白肉仔鸡饲粮的饲用效果。结果表明:以可消化氨基酸为基础研制的菜籽粕型无鱼粉肉仔鸡低蛋白平衡饲粮,可降低饲粮蛋白质水平1-1.5个百分点,试验鸡49日龄体重达2158.26克、料肉比2.15:1,增重和饲料转化率均达到或接近鱼粉型高蛋白饲粮的效果;每吨菜籽粕型无鱼粉饲粮可比鱼粉型饲粮降低原料成本72-8 相似文献
50.
对5个无核葡萄品种的试验表明,不同培养方式对离体胚发育的影响是很明显的,其中4个品种胚发育率液体培养高于固体培养,‘京早晶’在液体培养中胚发育率高达73.7%,是目前国内外胚发育率最高的报道;液体培养比固体培养胚的发育程度高,发育整齐,无畸形胚,易成苗. 相似文献