Oestrogen-induced bone marrow aplasia in a dog with a Sertoli cell tumour

A 10-year-old male Drahthaar dog with unilateral cryptorchidism was examined because of feminisation and myelotoxicity. Radiography and ultrasonography revealed an abdominal mass which was surgically removed. The mass was identified as a Sertoli cell tumour on histological examination. Findings on bone marrow examination were compatible with aplasia due to the oestrogens secreted by the tumoral cells. Treatment with fluids, antibiotics, whole blood transfusions, corticosteroids and lithium carbonate was unsuccessful. Survival time was 22 days after surgery.     

Growth and reproduction of the lion's paw scallop Nodipecten subnodosus in a suspended culture system at Guerrero Negro lagoon,Baja California Sur,Mexico

The lion's paw scallop, Nodipecten subnodosus (Sowerby) has considerable aquacultural potential due to its fast growth and large adductor muscle. Prior investigations throughout northwestern Mexico's littoral have reported highly variable growth rates; furthermore, no studies are available of the environment on growth and gametogenesis in this species under culture conditions. This investigation assesses the effect of food availability and temperature on the growth and gametogenesis of N. subnodosus in a suspended culture system at Guerrero Negro lagoon, Mexico. After 1 year of cultivation, N. subnodosus reached 69.13 mm in shell height (SH) (0.196 mm day^?1, 14 months old). Two significant growth spurts were observed: over the two first months of culture (August and September 2001, mean growth rate 0.4 mm day^?1) and in September 2002 (0.3 mm day^?1), both related to high temperatures and chlorophyll a concentrations. The onset of gametogenesis occurred in April 2002, with an increase in temperature (10‐month‐old scallops, 54.5 mm SH). The first spawning occurred in October and November (86.2 and 93 mm SH), with peak temperatures. These results, together with the analysis of previous reports, indicate that N. subnodosus has a higher preference for temperate areas; therefore, the Guerrero Negro lagoon appears to be a suitable site to culture this species.     

Typical and atypical manifestations of Anaplasma phagocytophilum infection in dogs

Eighteen clinically ill dogs, naturally infected with Anaplasma phagocytophilum, were examined at a veterinary practice in Baxter, Minnesota. A clinical examination, complete blood cell count, enzyme- linked immunosorbent assay (ELISA) for A phagocytophilum, Borrelia burgdorferi, and Ehrlichia canis antibodies and Dirofilaria immitis antigen, and a polymerase chain reaction test for A phagocytophilum DNA were obtained for all dogs. Physical examination findings included fever, arthropathy, lymphadenopathy, epistaxis, acute gastritis, cervical hyperpathia, and central nervous system dysfunction. Complete blood cell count abnormalities included thrombocytopenia, morulae in neutrophils, anemia, leukopenia, eosinopenia, lymphopenia, and monocytosis. Seroreactivity to A phagocytophilum was found in 61%, B burgdorferi antibodies in 17%, and D immitis antigen in 5% of the dogs. Fever, arthropathy, neurologic dysfunction, and epistaxis are clinical syndromes that can be associated with A phagocytophilum infection. Treatment with doxycycline resulted in rapid resolution of clinical signs in all dogs.     

Production of dairy cows fed whole-crop cereals or ryegrass silages supplemented with a fixed amount of concentrate

Abstract

Feed intake and milk production responses to whole-crop cereal silages and ryegrass silage (RGS) supplemented with a fixed amount of concentrate were measured in three 4×4 Latin square-designed experiment with three 17-day periods using 12 Holstein cows. Diets consisted of a fixed amount of concentrate (10 kg) and one of the following silages offered ad libitum: RGS, Mondego wheat silage (MWS), Alva wheat silage (AWS), and triticale silage (TS). Silage dry matter intake and milk yield were significantly higher for RGS. Milk composition was not affected by silage treatment. The whole-crop cereal silages tended to show higher milk concentrations of anteiso C15:0, iso C15:0, C16:1 and C18:1 and lower concentrations of C18:2 and C18:3. This study suggests better response of dairy cows to a single cut RGS than to whole-crop cereal silages harvested in an early stage of maturity.     

Biomass allocation and foliage heteroblasty in hard pine species respond differentially to reduction in rooting volume

Rooting space is considered as a resource in plants, but comparative studies on the biomass allocation plasticity in response to rooting volume (RV) are rare. We compared responses in growth, biomass allocation and ontogenetic heteroblasty in nine hard pine species of contrasted ecology. Seedlings were cultivated in containers of 0.2, 0.5, 1.0, 2.8 and 7 L for two growing seasons (425 days). Reduction in RV caused a reduction in plant absolute and relative growth rate and biomass allocated to stems but it increased biomass allocated to roots. RV affected to a lesser extent and in a less consistent direction allocation to leaves. Species that grew faster (higher relative growth rate) had a steeper decrease in growth with the reduction in RV. Ontogenetic heteroblasty, evaluated as the proportion of secondary needles in the needle biomass, showed highly different plasticity patterns in response to RV. Decrease in RV caused negligible or no change either in the most ontogenetically delayed Mediterranean pines or in the most ontogenetically advanced pines, the mesic Pinus sylvestris and P. uncinata. By contrast, ontogenetically intermediate species showed steep reaction norms in response to reduction in RV. While P. pinaster and P. brutia showed marked rejuvenation, P. nigra accelerated the development of adult foliage.     

Native pine species performance in response to age at planting and mulching in a site affected by volcanic ash deposition

Under heavily disturbed conditions, the selection of the appropriate native species and of planting and ameliorating techniques is necessary. Volcanic eruptions create harsh conditions that can preclude native plant establishment. We tested the performance of two native species Pinus pseudostrobus and P. montezumae for restoration of volcanic ash covered areas. Two age classes of P. pseudostrobus and one of P. montezumae were tested as well as the effect of mulching to ameliorate harsh substrate conditions. Results show that older plants of P. pseudostrobus (19-month old at planting) have higher survival and growth rates than young plants (8-months at planting). Plants at least 19-months-old at planting of P. pseudostrobus and P. montezumae, are appropriate for restoration of volcanic ash covered areas. Mulching had no effect on plant survival or growth for this experiment.     

Does afforestation increase bird nest predation risk in surrounding farmland?

Afforestation of agricultural land is increasingly used to deliver environmental benefits, but their effects on biodiversity remain poorly understood. This paper tests the hypothesis that afforestation changes predation processes in surrounding farmland, examining how the characteristics and landscape context of forest plantations affect predator (birds and mammalian carnivores) and key prey (rabbits and hares) abundances, and bird nest predation rates in Iberian cereal-steppes. Lagomorphs and predators were surveyed in fallow fields around 50 forest plantations, where predation rates were estimated using artificial nests set at 0, 100, 200 and 300 m from the forest edge. Recent plantations structurally similar to sparse (oak) or dense (pine) shrublands were associated with the highest hare and rabbit abundances, respectively, whereas both species avoided landscapes with high eucalyptus cover. In contrast, mature eucalyptus plantations showed strong positive effects on typical nest predators such as corvids and carnivores. Open farmland fragmentation favoured the abundance of lagomorphs and carnivores. Despite these effects and the high predation rate on artificial nests (49%), there was neither evidence for increased predation near plantation edges nor higher predation in fields with more lagomorphs and predators. However, predation tended to increase with cover by young oak plantations and overall forest plantation cover, to decrease with eucalyptus cover at both the local and landscape scales, and to peak in landscapes with intermediate edge densities. These results suggest that afforestation may have strong effects on bird nest predation rates by changing landscape composition and configuration, rather than by inducing local increases in predator and prey populations. Nevertheless, increased abundances of generalist predators associated with forest plantations may still be considered of conservation concern, thus supporting the recommendation for strongly restricting afforestation in areas important for open grassland birds. Where this is unavoidable, monitoring should be undertaken to provide early signals for bird population declines associated with predator increases, eventually triggering conservation action such as predator exclusion or removal.     

Phytochemical compounds involved in the anti-inflammatory effect of propolis extract

Two ethanolic propolis extracts (EPE) with and without the caffeic acid phenethyl ester (CAPE), CAPE and galangin (major components of propolis) were investigated for anti-inflammatory activity in rats using carrageenin foot oedema, carrageenin pleurisy and adjuvant arthritis. In our experiments, EPE with CAPE and CAPE alone significantly inhibited carrageenin oedema, carrageenin pleurisy and adjuvant arthritis. In contrast EPE without CAPE and galangin did not exhibit anti-inflammatory effects in acute and chronic inflammation. These results suggest that the anti-inflammatory activity of propolis is due to CAPE.     

Impact of Botryosphaeria,Diplodia and Neofusicoccum species on two Eucalyptus species and a hybrid: From pathogenicity to physiological performance

Species of Botryosphaeriaceae are associated with canker and dieback of Eucalyptus spp. worldwide, but little is known about their effect on the host physiology. The aim of this study was to evaluate the impact of Botryosphaeriaceae isolates from nine species in three genera (Botryosphaeria, Diplodia and Neofusicoccum), previously isolated from eucalypts, on three different Eucalyptus hosts (seedlings of E. nitens, cuttings of E. globulusand of E. globulus× E. cypellocarpa). An approach combining standard pathogenicity trials with evaluation of plant morpho‐physiological parameters was used. The size of the lesions produced revealed differences in fungal aggressiveness and host susceptibility. Isolates of Neofusicoccum kwambonambienseand Diplodia corticolawere the most aggressive, while Botryosphaeria dothidea and Diplodia seriataisolates were the least aggressive. In general, hybrid E. globulus× E. cypellocarpa plants developed smaller lesions, followed by E. nitens and E. globulus. Eucalyptus nitensplants showed minimal modifications on the morpho‐physiological profile when infected, although more severe symptoms and mortality were observed. This is probably due to a more variable genetic background of the plants. However, in general, no direct association between putative fungal aggressiveness and plant physiological disorders could be found. Results suggested that under optimal growth conditions plants manage to cope with pathogen attack and maintain their physiological performance.