Plant growth-promoting rhizobacteria: strategies to improve abiotic stresses under sustainable agriculture

The abiotic stresses like drought, heavy metal and salts directly or indirectly influence the global environmental pollution and decrease the agricultural productivity. The stress tolerant plant growth promoting rhizobacteria (PGPR) play an important role against the abiotic stresses in terms of enhancing the efficacy of soil, plant growth promotion (PGP). Stress tolerance PGPRs have certain specific PGP properties such as hormones synthesis, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, Indole-3-acetic acid (IAA) production, Abscisic acid (ABA) synthesis, enzymes production, nitrogen fixation, phosphorus (P) and Potassium (K) solubilization, as results which protect various crops during such stress conditions and consequently enhance crop sustainability. Efficient PGPRs isolated from various stress conditions have certainly, more useful against that particular stress. This article highlighted the isolation of various stresses tolerant PGPRs from varieties of crops under different stress conditions and their effect on varieties of crops to enhance their plant growth promotion.     

Latent heat flux estimation in clear sky days over Indian agroecosystems using noontime satellite remote sensing data

A simplified evaporative fraction (Λ) based single-source energy balance scheme was tested with moderate resolution (1 km) noontime satellite observations to evaluate clear sky latent heat flux (λE) estimates over diverse agricultural landscapes. This approach uses two-dimensional (2D) scatter between land surface temperature (LST) and albedo to determine Λ. The operational utility of this scheme was demonstrated for estimating regional evapotranspiration and consumptive water use during rabi (November to April) crop growing season to predict pre-harvest wheat yield (error within 15.9% of reported mean) using time series data. The existence of triangular relations between Λ and LAI (leaf area index) or NDVI (normalized difference vegetation index) was found with basal line (hypotenuse) linearly coupled with LAI or NDVI at low level of surface soil wetness. The analysis of diurnal course of in situ Λ proved the validity of constant-Λ hypothesis over pure, uniform, homogeneous crop canopies but showed irregular and wave-like patterns over heterogeneous, mixed crop canopies. The root mean square error (RMSE) of noontime and daytime average λE estimates with respect to in situ λE measurements were also smaller over homogeneous agricultural canopies (41 and 23 W m⁻²) with correlation coefficients (r) 0.94 and 0.96, respectively, from 135 clear sky datasets as compared to RMSE over heterogeneous ones (59 and 28 W m⁻² with r = 0.66 and 0.82, respectively from 22 datasets). The intercomparison with another Λ based approach (LST–NDVI 2D scatter) showed the supremacy of Λ determined from LST–albedo 2D scatter. The efficiency of LST–NDVI scatter was better during the dry down or water limited phases of crop growth only. The uncertainties of λE estimates were attributed to errors in core radiation budget inputs, relative loss of conservativeness of Λ due to canopy heterogeneity, and the inherent limitations of the single-source approach. There is further scope to reduce present λE uncertainties by combining the new findings on Λ (LST–albedo scatter)–NDVI triangular relations, diurnal Λ and two-source radiation budget.     

Dynamics and modeling of soil water under subsurface drip irrigated onion

Subsurface drip irrigation provides water to the plants around the root zone while maintaining a dry soil surface. A problem associated with the subsurface drip irrigation is the formation of cavity at the soil surface above the water emission points. This can be resolved through matching dripper flow rates to the soil hydraulic properties. Such a matching can be obtained either by the field experiments supplemented by modeling. Simulation model (Hydrus-2D) was used and tested in onion crop (Allium cepa L.) irrigated through subsurface drip system during 2002-2003, 2003-2004 and 2004-2005. Onion was transplanted at a plant to plant and row to row spacing of 10 cm × 15 cm with 3 irrigation levels and 6 depths of placement of drip lateral. The specific objective of this study was to assess the effect of depth of placement of drip laterals on crop yield and application of Hydrus-2D model for the simulation of soil water. In sandy loam soils, it was observed that operating pressures of up to 1.0 kg cm⁻² did not lead to the formation of cavity above the subsurface dripper having drippers of 2.0 l h⁻¹ discharge at depths up to 30 cm. Wetted soil area of 60 cm wide and up to a depth of 30 cm had more than 18% soil water content, which was conducive for good growth of crop resulting in higher onion yields when drip laterals were placed either on soil surface or placed up to depths of 15 cm. In deeper placement of drip lateral (20 and 30 cm below surface), adequate soil water was found at 30, 45 and 60 cm soil depth. Maximum drainage occurred when drip lateral was placed at 30 cm depth. Maximum onion yield was recorded at 10 cm depth of drip lateral (25.7 t ha⁻¹). The application of Hydrus-2D confirmed the movement of soil water at 20 and 30 cm depth of placement of drip laterals. The model performance in simulating soil water was evaluated by comparing the measured and predicted values using three parameters namely, AE, RMSE and model efficiency. Distribution of soil water under field experiment and by model simulation at different growth stages agreed closely and the differences were statistically insignificant. The use of Hydrus-2D enabled corroborating the conclusions derived from the field experimentation made on soil water distribution at different depths of placement of drip laterals. This model helped in designing the subsurface drip system for efficient use of water with minimum drainage.     

Three-dimensionally ordered array of air bubbles in a polymer film

We report the formation of a three-dimensionally ordered array of air bubbles of monodisperse pore size in a polymer film through a templating mechanism based on thermocapillary convection. Dilute solutions of a simple, coil-like polymer in a volatile solvent are cast on a glass slide in the presence of moist air flowing across the surface. Evaporative cooling and the generation of an ordered array of breath figures leads to the formation of multilayers of hexagonally packed water droplets that are preserved in the final, solid polymer film as spherical air bubbles. The dimensions of these bubbles can be controlled simply by changing the velocity of the airflow across the surface. When these three-dimensionally ordered macroporous materials have pore dimensions comparable to the wavelength of visible light, they are of interest as photonic band gaps and optical stop-bands.     

Scanning electron, light, and immunofluorescent microscopy of coronaviral enteritis of turkeys (Bluecomb).

Intestinal sections from both experimental and field cases of turkey coronaviral enteritis (TCE) were examined by scanning electron microscopy and light microscopy through 10 days after inoculation and by a direct fluorescent antibody test for TCE through 12 days. Serums were collected for an indirect fluorescent antibody test for TCE through 160 days after inoculation. Lesions observed with the scanning electron microscopy were catarrhal enteritis with hemorrhage per diapedesis, epithelial desquamation, and villous atrophy which developed and regressed within 6 days after inoculation. Light microscopy demonstrated similar lesions, except that villus-to-crypt ratios remained depressed 10 days. The direct fluorescent antibody test demonstrated the presence of coronaviral antigen throughout the sampling period, and serum antibodies to TCE were present until at least 160 days, when the experiment was terminated.