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排序方式: 共有112条查询结果,搜索用时 15 毫秒
1.
Pankaj Sharma Inderjit Singh Asmita Sirari Gaurav Khosla Gurjeet Singh Navkiran Kaur Ludhar Sarvjeet Singh 《Plant Breeding》2019,138(6):741-747
Inheritance of fertility restorer gene in pigeonpea was studied using F2 and BC1F1 populations derived from cross AL103A × IC245273. It was found to be controlled by single dominant gene. Out of 228 SSR primer pairs, 33 primer pairs showed parental polymorphism, while four primers were found polymorphic in bulk segregant analysis (BSA). These four primers viz., CcM 1615, CcM 0710, CcM 0765 and CcM 1522 were used for genotyping of F2 population and were found to be placed at 3.1, 5.1, 28.1 and 45.8 cM, respectively. Two of them, CcM 1615 and CcM 0710, evinced clear and unambiguous bands for fertility restoration in F2 population. The Rf gene was mapped on linkage group 6 between the SSR markers CcM 1615 and CcM 0710 with the distances of 3.1 and 5.1 cM, respectively. The accuracy of the CcM 1615 was validated in 18 restorers and six maintainer lines. The marker CcM 1615 amplified in majority of male restorer lines with a selection accuracy of 91.66%. 相似文献
2.
A. N. Mishra K. Kaushal G. S. Shirsekar S. R. Yadav R. N. Brahma H. N. Pandey 《Plant Breeding》2005,124(5):514-516
The bread wheat cultivar ‘Thatcher’ is documented to carry the gene Lr22b for adult‐plant resistance to leaf rust. Seedling‐resistance to leaf rust caused by Puccinia triticina in the bread wheat cultivar ‘Thatcher’, the background parent of the near‐isogenic lines for leaf rust resistance genes in wheat, is rare and no published information could be found on its genetic basis. The F2 and F3 analysis of the cross ‘Agra Local’ (susceptible) × ‘Thatcher’ showed that an apparently incompletely dominant gene conditioned seedling‐resistance in ‘Thatcher’ to the three ‘Thatcher’‐avirulent Indian leaf rust pathotypes – 0R8, 0R8‐1 and 0R9. Test of allelism revealed that this gene (temporarily designated LrKr1) was derived from ‘Kanred’, one of the parents of ‘Thatcher’. Absence of any susceptible F2 segregants in a ‘Thatcher’ × ‘Marquis’ cross confirmed that an additional gene (temporarily designated LrMq1) derived from ‘Marquis’, another parent of ‘Thatcher’, was effective against pathotype 0R9 alone. These two genes as well as a second gene in ‘Kanred’ (temporarily designated LrKr2), which was effective against all the three pathotypes, but has not been inherited by ‘Thatcher’, seem to be novel, undocumented leaf rust resistance genes. 相似文献
3.
A. N. Mishra K. Kaushal S. R. Yadav G. S. Shirsekar H. N. Pandey 《Plant Breeding》2005,124(5):517-519
The gene Lr34 has contributed to durable resistance to leaf rust caused by Puccinia triticina in wheat worldwide. The closely associated leaf tip necrosis is generally used as the gene's marker. Lr34 has been postulated in many Indian bread wheat cultivars including ‘C 306’, based on the associated leaf tip necrosis and a few other field and glasshouse observations. The present study showed monogenic control of adult‐plant resistance in ‘C 306’ to leaf rust pathotype 77‐5 (121R63‐1). The F2 segregation in the crosses between ‘C 306’ and the two known carriers of Lr34, ‘Line 897’ and ‘Jupateco 73’‘R’ fitted a digenic ratio. The F3 families derived from the susceptible F2 segregants were true breeding for susceptibility, proving the absence of Lr34 in ‘C 306’. The cross between ‘Line 897’ and ‘Jupateco 73’‘R’ did not segregate for susceptibility. Resistance in the cross ‘Agra Local’ (susceptible) × ‘C 306’ was associated with leaf tip necrosis, showing that the leaf rust resistance gene in ‘C 306’ was associated with leaf tip necrosis, but was different from Lr34. This gene is being temporarily designated as Lr‘C 306’. Hence, leaf tip necrosis cannot be considered as an exclusive marker for selecting Lr34 in wheat improvement. 相似文献
4.
Identification of a diverse mini‐core panel of Indian rice germplasm based on genotyping using microsatellite markers
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Kapil K. Tiwari Anshuman Singh Sasmita Pattnaik Maninder Sandhu Sukhdeep Kaur Sourabh Jain Sushma Tiwari Shweta Mehrotra Mahender Anumalla Rashmita Samal Jyoti Bhardwaj Neha Dubey Vikrant Sahu Gayle A. Kharshing Patu K. Zeliang Kadiri Sreenivasan Pankaj Kumar Swarup K. Parida Sevanthi V. A. Mithra Vandana Rai Wricha Tyagi Pawan K. Agrawal Atmakuri R. Rao Arunava Pattanayak Girish Chandel Ashok K. Singh Ishwari S. Bisht Kangila V. Bhat Gundimeda J. N. Rao Jitendra P. Khurana Nagendra K. Singh Trilochan Mohapatra 《Plant Breeding》2015,134(2):164-171
Identification of a small core germplasm set representing the available genetic diversity is essential for its proper evaluation and subsequent utilization in rice improvement programmes. For constituting a small diverse mini‐core panel of Indian rice germplasm, a representative set of 6912 accessions drawn based on their geographic origin from the whole rice germplasm collection available in the National Gene Bank was genotyped using 36 microsatellite markers. Automated fragment analysis of amplicons yielded a total of 435 alleles, with an average 12.4 and range of 3–29 alleles per locus. Polymorphism information content (PIC) ranged from 0.08 (RGNMS190) to 0.86 (RM552) with an average of 0.528. Based on genotyping data, a mini‐core consisting of 98 genotypes was identified. Ninety‐four per cent of the alleles present in the core set were present in the mini‐core. The identified small but diverse panel will be useful for further intensive trait‐specific evaluation and utilization in allele mining. 相似文献
5.
A. N. Mishra K. Kaushal S. R. Yadav G. S. Shirsekar H. N. Pandey 《Plant Breeding》2005,124(5):520-522
Recessively inherited gene Sr2 has provided the basis of durable resistance to stem rust (caused by Puccinia graminis tritici) in wheat (Triticum aestivum L.) worldwide. The associated earhead and stem melanism or ‘pseudo‐black chaff’ is generally used as a marker for this gene. Sr2 has been postulated in many wheat cultivars of India including ‘Lok 1’, based on associated pseudo‐black chaff in adult plants, and leaf chlorosis in seedlings. However, dominant inheritance of the resistance factor operating in ‘Lok 1’, and a 13 : 3 (resistant : susceptible) F2 segregation in the ‘Sr2‐line’ (‘Chinese Spring’6 × ‘Hope’ 3B) × ‘Lok 1’ cross confirmed that Sr2 was absent in ‘Lok 1’. Susceptible plants with a pseudo‐black chaff phenotype were observed in F2 populations of ‘Agra Local’ (susceptible) × ‘Lok 1’, and the ‘Sr2‐line’ × ‘Lok 1’ crosses. Most of the F3 families derived from the susceptible F2 segregants with pseudo‐black chaff phenotypes were true breeding for the expression of pseudo‐black chaff with susceptibility to stem rust. Thus, linkage of pseudo‐black chaff with Sr2 in wheat can be broken, and hence, caution may be exercised in using pseudo‐black chaff as a marker for selecting Sr2 in breeding programmes. 相似文献
6.
Introduction
Wood quality is an important criterion in the selection of superior genotypes when breeding for solid wood. 相似文献7.
Feeding and casting activity of Amynthas alexandri fed on corn, wheat leaves, and mixed grasses were monitored in laboratory cultures. Casts were produced on the surface and sides of the containers. Food consumption varied from 36.5 to 69 mg g–1 live worm day–1. Cast production ranged from 3.95 to 5.9 mg g–1 live worm day–1. The C:N ratio in casts in laboratory cultures (11.17) and in field samples (8.84) was consistently lower than the corresponding ratio in the parent soil (13.19 and 10.54, respectively). This was probably due to mineralization of plant-derived organic material during passage through earthworms with consequent low C:N ratios. 相似文献
8.
9.
Abha Jain A. K. Roy P. Kaushal D. R. Malaviya S. N. Zadoo 《Genetic Resources and Crop Evolution》2006,53(2):339-347
Isozyme banding pattern was studied in Guinea grass (Panicum maximum Jacq.), a widely cultivated grass having good fodder value. Similarity among 63 accessions collected from diverse sources
was worked out using five enzyme systems (SOD, GOT, ACP, Esterase and Peroxidase) following horizontal starch gel electrophoresis.
Biochemical markers such as isozymes are useful supplements in identifying the genetic variation present in any crop. A total
of 35 clear and unambiguous bands were used for analysis of which 8 bands were monomorphic. Polymorphism exhibited by 27 bands
from all five enzyme systems indicate presence of considerable diversity in this species. The dendrogram generated after UPGMA
and SAHN cluster analysis using Jaccard genetic distance showed that 63 accessions from diverse geographical locations could
be grouped in three main clusters, of which two could further be divided into sub-clusters. Although the clusters comprised
members from different locations, most of the accessions from similar geographical locations tended to cluster in same group. 相似文献
10.
Shibata Y Kumar P Layer R Willcox S Gagan JR Griffith JD Dutta A 《Science (New York, N.Y.)》2012,336(6077):82-86
We have identified tens of thousands of short extrachromosomal circular DNAs (microDNA) in mouse tissues as well as mouse and human cell lines. These microDNAs are 200 to 400 base pairs long, are derived from unique nonrepetitive sequence, and are enriched in the 5'-untranslated regions of genes, exons, and CpG islands. Chromosomal loci that are enriched sources of microDNA in the adult brain are somatically mosaic for microdeletions that appear to arise from the excision of microDNAs. Germline microdeletions identified by the "Thousand Genomes" project may also arise from the excision of microDNAs in the germline lineage. We have thus identified a previously unknown DNA entity in mammalian cells and provide evidence that their generation leaves behind deletions in different genomic loci. 相似文献