普通刺猬的解剖学研究(二) 骨骼

对28只（♀16,♂12）成年普通利猬（Erinaceuseuropaeus）的骨骼标本进行了解剖学研究,结果表明,普通刺猬全身骨骼有316～317枚,其中头骨48枚,脊椎骨43～44杖,椎式为C7T15L6S3Cy12～13,肋骨15对,胸骨5枚,前肢骨每侧47杖,后肢骨每侧48枚。普通刺猬骨骼存在有性别差异并表现出与刺猬生活习性相适应的一些特征。     

Promoter anchored amplified polymorphism based on random amplified polymorphic DNA (PAAP-RAPD) in cotton

Non-coding sequences account for a majority of the higher plant genome, some of which have important effects in gene regulation and plant development. In an effort to develop molecular marker systems to search for polymorphisms associated with high fiber yield and quality in cotton, we have developed a methodology that could specifically target the regulatory regions of the cotton genome. In this study we designed 10-nucleotide degenerate promoter primers based on conserved core promoter sequences and tested their applicability in PCR amplifications in combination with 10-mer random amplified polymorphic DNA (RAPD) primers. The amplified markers are called promoter anchored amplified polymorphism based on RAPD (PAAP-RAPD). Forty cotton genotypes with diverse genetic and geographical backgrounds were used to test the PAAP-RAPD system using polyacrylamide gel electrophoresis. Based on PAAP-RAPD markers amplified from 12 primer combinations, the 40 genotypes were classified into five distinctive groups: two Upland cotton (Gossypium hirsutum) groups from China, another two Upland cotton groups from the USA, and one group from American Pima cotton (G. barbadense). The groupings are in general consistent with their genetic and geographical origins. Thirty-six PAAP-RAPD and RAPD fragments were cloned and four of them were further subjected to sequence analysis. Signal scanning using software PLACE confirmed that they contained an array of cis-regulatory sequences in addition to the core promoter sequences. The results demonstrate the potential application of PAAP-RAPD as a new marker system specifically targeting regulatory regions of the plant genome.     

夏橙果皮天然蜡和人工涂蜡表面的观察

 用扫描电子显微镜(SEM) 观察了有天然蜡和商业涂覆蜡存在的夏橙果实表面,并研究了天然蜡、洗果、脱蜡和商业涂覆蜡对果实失重的影响。结果表明: 夏橙果实表面的蜡片形状和大小均不规则, 大部分可用氯仿- 甲醇溶液去除; 除蜡极易加大果实失水; 涂蜡可部分和全部堵塞果实表面气孔。     

槽式堆肥中搅拌螺旋输送能力的研究

针对深槽式堆肥系统的搅拌螺旋物料输送能力展开研究.在特定的工作倾角下,调整搅拌螺旋的旋转速度和横向移动速度,计量物料的输送状态.实验表明:在不同的横向运动方向上,搅拌螺旋的输送能力不同;较高的转速和较低的横向移动速度有利于物料输送.     

痣鳞鳃金龟的生物学特性与防治

痣鳞鳃金龟二年发生一代 ,此虫在砂质土壤的林地密度大 ,为害严重。造林时在每株根际施 5g 3 %呋喃丹颗粒剂 ,可有效地控制此虫的危害并兼治其它地下害虫和造林初期的食叶害虫。     

SINPV PK基因的部分序列分析

重组质粒ｐＰ１８ＰＨ３上含有ＳＩＮＰＶ的部分蛋白激酶基因。ＳＩＮＰＶ部分的蛋白激酶氨基酸序列与ＨａＮ－ＰＶ、ＨｚＮＰＶ、ＳｐｌｉＮＰＶ、ＡｆＮＰＶ、ＡｃＮＰＶ、ＬｄＮＰＶ蛋白激酶的氨基酸序列的同源性分别为４５％、８９％、３７％、４４％、３８％和３７％，其上含有蛋白激酶特征序列ＩＶＨＡＮＤＶＫＬＥＮＶＬ。     

YTG型双向体积管在计量检定中的应用

体积管是检定流量计的标准器。ＹＴＧ型双向体积管，其外型尺寸、主体结构、检定速度、日常管理等方面，均比单向体积管有明显的优点，并具有独特的容积补偿性，给体积管的管理、使用带来极大方便。介绍了ＹＴＧ型双向体积管的结构、技术指标及其优越性，并建议在条件允许的情况下，尽可能使用双向体积管。     

Myocardial and pancreatic lesions induced by T-2 toxin, a trichothecene mycotoxin, in swine

Myocardial and pancreatic lesions induced by sublethal doses of T-2 toxin in swine were characterized by light and electron microscopy. Toxin was given intravenously to six 17- to 18-week-old pigs. Pigs were killed 24 or 48 hours after treatment. Grossly, subendocardial hemorrhages, multifocal pinpoint white foci in myocardium, and pancreatic edema occurred in one treated pig. Histologic changes in myocardium of treated pigs consisted of multifocal edema, mononuclear cell infiltration, myofiber hyalinization, vacuolation, and contraction bands with nuclear pyknosis. Ultrastructurally, there were areas of edema, myofibrillar disorganization, dilation of sarcoplasmic reticulum, and formation of hypercontraction bands. Myocardial mineralization was seen in the pig with gross lesions. Pancreatic changes in treated pigs consisted of multifocal acinar degeneration and necrosis. Ultrastructural changes included irregular dilation of rough endoplasmic reticulum and abnormal zymogen granules. Thus, in addition to radiomimetic lesions of the gastrointestinal tract and lymphoid organs, heart and pancreas are target organs of T-2 toxin in swine.