去势和热应激对筠连黄牛生产性能、瘤胃发酵和血液生化指标的影响

为研究去势和热应激对筠连黄牛生产性能、瘤胃发酵和血液生化指标的影响,本研究选用18头16月龄、体重相近((292.35±28.71) kg)的不同去势程度筠连黄牛(全去势FCG、半去势HCG、假手术SOG),分为3组,每组6个重复,每个重复1头牛,根据试验期间牛舍温湿度指数(THI),分为热应激期(7、8月)和非热应激期(9月),分析去势和热应激对黄牛各指标的影响。结果表明：1)与非热应激期相比,热应激期牛舍THI、肉牛呼吸频率和直肠温度均显著提高(P<0.05)。2)去势和热应激均显著提高血清热休克蛋白70、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)和雌二醇浓度(P<0.05),而睾酮、甲状腺素(T4)、胰岛素和游离脂肪酸浓度显著降低(P<0.05),且除血清葡萄糖、T4和LDL-C外,去势和热应激对其他血清指标存在显著互作效应(P<0.05)。3)全去势和热应激均显著降低肉牛平均日增重(ADG),且存在显著互作效应(P<0.05)。4)热应激期,去势显著降低粗蛋白、酸性洗涤纤维和钙表观消化率以及血清总抗氧化能力(T-AOC)(P<0....     

气吸式杂交稻单粒排种器研制

针对气力式水稻精量排种器充种不稳定、单粒播种精度不高和播种量大的问题,该研究设计了一种具有矩形吸种孔和辅助充种装置的气吸式杂交稻单粒排种器。根据“吉田优”型杂交稻的长短轴重力分布情况,确定排种盘吸种孔形状;基于CFD-DEM(Computational fluid dynamics, Discrete element method)流固耦合理论,以吸附力为指标,进行5类具有相同面积的吸种孔单因素仿真试验,确定吸附力最大的吸种孔规格为0.8 mm×2.25 mm;以该型吸种孔为基础,选取辅助充种角、工作转速和工作负压为试验因素,以单粒率S、多粒率M和漏播率L为试验指标,开展Box-Bhnken台架试验,对试验结果进行响应曲面分析和多目标优化,得到排种盘辅助充种角为80.90°、工作转速为42.65 r/min、工作负压为621 Pa时,排种器的单粒率为86.91%,漏播率为3.63%。验证试验结果的排种器单粒率为86.36%、漏播率为3.41%,与优化结果吻合。研究结果可为后续气吸式杂交稻单粒排种器的优化设计和直播机整机作业精度的提高提供指导。     

黄泥田晚稻肥料效应的研究

肥料是作物增产的重要因素。在农田施肥实践中,氮、磷、钾肥最佳用量及合理配比因土壤不同而不同。为此,采用3个因子4、个不同施肥用量水平,展开了黄泥田氮磷钾肥料效应的试验,推荐了最佳施肥用量。     

对虾暴发性疾病及防治对策

本文根据几年来虾病研究工作的进展和江苏对虾流行病学调查结果，总结分析了病因及病原学方面的有关问题，并针对水温、气候、密度等诱发因子，从生态角度提出了虾病防治方法及措施。     

Identification of serum differential proteins in colorectal adenoma and early malignantly transformed adenoma by proteomics

AIM: To investigate the associated proteins and sensitive biomarkers for early diagnosis of colorectal adenocarcinoma by comparing the results of differential proteomic analysis between colorectal adenoma and early malignantly transformed adenoma. METHODS: Two-dimensional gel electrophoresis was used to define patterns of protein expressions of colorectal adenoma and early malignantly transformed adenoma. Proteins expressed differentially among groups were detected, cut out and analyzed by MALDI-TOF/TOF mass spectrometry. RESULTS: Two-dimensional protein maps of colorectal adenoma and early malignantly transformed adenoma were analyzed with gel-analysis software, an average of 1 672 spots in adenoma, 1 732 in early malignantly transformed adenoma were observed. 28 spots of a 1.5-fold change were found, including 15 proteins down-regulated and 13 up-regulated in early malignantly transformed adenoma, in which 23 proteins were identified by mass spectrometry, the rate of identification was 82.14%. 13 differential proteins were attained, 8 were up-regulated and 5 were down-regulated, which was classified to 6 categories, including protease inhibitor, complement, immunoglobulin, keratoproteins, signal transduction protein and function-unknown proteins. CONCLUSION: The changes of serum proteins in early malignantly transformed adenoma from adenoma can be identified by proteomic technology. Proteins detected in the study may provide new biomarkers correlated with biological behavior of colorectal adenocarcinoma.     

Role of nestin in the maintenance of the structure of podocytes

AIM:To investigate the expression of nestin, a kind of cytoskeletal protein in cultured murine podocytes and the role of nestin in the maintenance of the podocyte structure.METHODS:The immortalized murine podocytes were cultured. The expression of nestin was determined by immunofluorescence. In differentiated podocytes, the expression of nestin was knock-down by RNAi. The effect of nestin knock-down was examined by Western blotting and immunofluorescence. The projections longer than maximal length of the cell body in which the cells transfected with siRNA and control vector that contained nonhomologous oligo were counted, respectively. RESULTS:Nestin siRNA markedly reduced or abolished nestin expression. In cells transfected with nestin siRNA, the percentage of cells with processes was significantly lower than that in cells transfected with control vector (77.0%±6.3% vs 16.0%±4.6%, n=3, P<0.01). CONCLUSION:Nestin may play an important role in maintaining normal function of podocytes.     

Effects of apolipoprotein (a) on vascular smooth muscle cells proliferation and cell signal transduction pathway

AIM: To investigate the effect and the mechanism of apolipoprotein (a) ［apo (a)］ on proliferation of vascular smooth muscle cells (VSMCs). METHODS: All VSMCs used in experiments were serial subcultured from primary cells and were identified by immunohistochemistry staining of α-actin. Cell growth assay was observed as cell counting and MTT assay. Western blotting was also employed to detect the related mechanism. RESULTS: All cells used in experiments were confirmed as VSMCs. Although apo (a) enhanced VSMCs proliferation, this effect was attenuated by anti-integrin αⅤβ3, LM609. Use these reagents alone had no effect on VSMCs growth. The results of Western blotting demonstrated that focal adhesion kinase (FAK) was activated by apo (a) and the expression of total or phosphorylated transforming growth factor β1 (TGF-β1) was also decreased. However, these effects described above were all blocked by LM609. CONCLUSION: Apolipoprotein (a) enhances VSMCs proliferation and this effect is mediated by integrin αⅤβ3, which activates FAK and attenuates TGF-β1 and phospho-TGF-β1 expression.     

不同基质及生根剂浓度对五种园林植物扦插生根的影响

以5种园林植物为材料,进行不同基质与不同生根剂浓度对植物扦插生根的比较研究.结果表明:月季、大叶黄杨最适宜的基质是河沙,小叶女贞、八角金盘最适宜的基质是珍珠岩,鹅掌柴最适宜的基质是混合基质.月季、大叶黄杨最合适的生根剂浓度为3 000 mg/kg,小叶女贞最合适的生根剂浓度为5000mg/kg,八角金盘、鹅掌柴最合适的生根剂浓度为1000mg/kg.     

中小果型无籽西瓜新品种博帅的选育

博帅系湖南博达隆科技发展有限公司以9048为母本、M-2为父本杂交育成的中小果型黄瓤无籽西瓜新品种。该品种全生育期90d左右,雌花开放至果实成熟约需27d。植株生长势中强,抗病抗逆性强。果实短椭圆形,果形指数1.2。果皮浅绿底覆绿色齿条带,厚度0.9cm,硬韧,耐贮运。瓤色鲜黄,质脆爽口,纤维少,无籽性能好;中心可溶性固形物12%～13%,边部9%～9.5%。单果质量4kg左右,667m2产量2700kg左右。适应性广,全国各地均可栽培。2009年3月通过湖南省农作物品种审定委员会审定,审定编号:湘审西瓜2009002。