Trichostatin A and nuclear reprogramming of cloned rabbit embryos

To investigate the influence of histone deacetylases on nuclear reprogramming after nuclear transfer, we treated the cloned embryos with a histone deacetylase inhibitor, Trichostatin A (TSA). In the present study, global changes in acetylation of histone H3-lysine 14, histone H4-lysine 12, and histone H4-lysine 5 were studied in rabbit in vivo fertilized embryos, somatic cell nuclear transfer (SCNT) embryos, and TSA-treated SCNT embryos. From the pronuclear to the morula stage, the deacetylation-reacetylation changes in acetylation of histone H3-lysine 14 and histone H4-lysine 12 occurred in both fertilized embryos and TSA-treated cloned embryos; however, the distribution pattern in untreated cloned embryos failed to display such changes. More interesting, the signal of acetylation of histone H4-lysine 12 in cloned embryos was detected in both the inner cell mass and the trophectoderm, whereas TSA-treated cloned embryos showed the same staining pattern as fertilized embryos and the staining was limited to the inner cell mass. The histone acetylation pattern of TSA-treated SCNT embryos appeared to be more similar to that of normal embryos, indicating that TSA could improve nuclear reprogramming after nuclear transfer.     

Energy gaps in "metallic" single-walled carbon nanotubes

Metallic single-walled carbon nanotubes have been proposed to be good one-dimensional conductors. However, the finite curvature of the graphene sheet that forms the nanotubes and the broken symmetry due to the local environment may modify their electronic properties. We used low-temperature atomically resolved scanning tunneling microscopy to investigate zigzag and armchair nanotubes, both thought to be metallic. "Metallic" zigzag nanotubes were found to have energy gaps with magnitudes that depend inversely on the square of the tube radius, whereas isolated armchair tubes do not have energy gaps. Additionally, armchair nanotubes packed in bundles have pseudogaps, which exhibit an inverse dependence on tube radius. These observed energy gaps suggest that most "metallic" single-walled nanotubes are not true metals, and they have implications for our understanding of the electronic properties and potential applications of carbon nanotubes.     

关于中国土壤系统分类中变性土分类研究的商榷

中国土壤系统分类(二稿),已于1987年3月问世,其中有许多宝贵的信息和进展。根据1979年以来我们对中国变性土和变性的土壤(vertic soils)的广泛调查和研究,谨对二稿中有关变性土的诊断特征,亚纲、土类和亚类的划分标准和依据等提出了见解,可作进一步拟定变性土系统分类的参考,文中还强调了完善中国变性土系统分类有待研究的问题。     

一套GPS两种功能的结合在水土保持监测中的应用研究

通过试验与分析,利用一套双频GPS进行单点定位与快速RTK技术的结合来快速测量水土流失面积,即:先用GPS单点适时定位完成控制测量,再利用RTK技术测量水土流失的边界,当超出RTK测量的有效范围时,再采用单点定位法进行控制点的加密,以便于继续RTK测量工作,直至测区边界的测量工作全部完成为止。这种方法简单、准确、经济,解决了水土保持监测中的面积度量问题,克服了由于国家三角点被破坏而受数量与分布的限制的缺点。     

施肥方法对烤烟氮磷钾利用率及产量品质的影响

1991年在零陵、郴州烟区采用田间试验研究了肥料深施与浇施及基肥与追肥分配比例对烤烟氮、磷、钾利用率及产量品质的影响.结果表明,以氮、钾肥全作追肥浇施处理,肥料利用率,烟叶产量及产值最高,品质也较好;20%氮、钾作追肥深施,80%作追肥浇施处理次之;而将氮、钾肥及有机肥全部深施处理的效果较差.     

Estimating Combining Ability of 4x-2x Crosses in Cultivated Potatoes

Progenies derived from crosses between Solarium tuberosum and 2n pollen-Producing diploid hybrids, exhibit obvious hybrid vigor. The 2n pollen-producing clone can act as a bridge in crossing S. tuberosum and S. andigena with S. phureja. Populations from 4x-2x crosses show more unifomity and less segregation compared with that of 4x-4x crosses. The parent-offspring correlation for the traits, starch content and tuber number, is significant at 0.01 level. The regrssion equations are Y (mp-F1)=1.0 1.2x and Y (mp - F1) = 5.3 0.8x, respectively. The 2n pollen-producing clones play an impotant role in increasing tuber stach content. Estimates of the combining ability for the main yield components indicate that additive effect prodominatcs for such trais as plot yield, tuber weight per plant and starch content, whereas both additive and non-additive effects lay equal stress on mean tuber weight and non-additive effect is important for tuber number. In general, non-additive effect appears to be important in     

孟宗沟小流域综合治理途径的探讨

该从小流域水土流失的原因出发，探讨了长江源头区水土保持工作的正确指导思想，治理水土流失的方法，步骤，技术措施及综合治理与农牧业发展的关系，探索出高寒边远少数民族牧区脱贫致致富的途径，为长江上游水土流失区的综合治理提供经验和科学依据。     

Investigation of Co-infection of PPV7 and PCV2 and Genetic Variation Analysis for PPV7 Cap Gene in Fujian and Guangdong

【Objective】 To investigate the co-infection situation of Porcine parvovirus (PPV7) and Porcine circovirus type 2 (PCV2)in Fujian and Guangdong, and to understand the molecular genetic characteristics of PPV7 Cap gene.【Method】 The blood sample of 432 infected pigs from 69 pig farms in Fujian and Guangdong were collected to detect PPV7 and PCV2 by PCR.The PPV7 Cap gene of positive samples was cloned and sequenced.The DNAStar software was used to analyze the nucleotide and amino acid sequences of PPV7 Cap gene, and Mega 7.0 software was used to draw the genetic evolution tree.【Result】 The results showed that the positive rate of PPV7 was 21.99% (96/432), the positive rate of farms was 53.62%(37/69), and the positive rate of PCV2 was 54.17% (234/432), and the co-infection rate of PCV2 and PPV7 was 13.43% (58/432).The 17 PPV7 Cap gene sequences were amplified using PCR.Nucleotide homology analysis revealed that the homology of the 17 PPV7 Cap gene sequences was 85.6%-100%, and the homology with reference strains was 85.8%-99.0%.Amino acid sequence comparison analysis revealed that the amino acid homology of the 17 PPV7 Cap protein sequences was 87.6%-100%, and the amino acid homology with reference strains was 82.6%-98.7%.Phylogenetic analysis of Cap gene showed that PPV7 could be divided into five main evolutionary branches of PPV7a-PPV7e, among which 9 isolates belonged to PPV7a subtype, 3 isolates belonged to PPV7b subtype, 4 isolates belong to PPV7c subtype, and only 1 isolates isolates belonged to PPV7e subtype.【Conclusion】 This study indicated that PPV7 was widely prevalent in Fujian and Guangdong regions, and had a high co-infection rate with PCV2, which might be the pathogenic factor of Porcine circovirus associated disease(PCVAD).The genetic diversity of PPV7 isolates was abudant in both regions, and PPV7a was the dominant strain at present.The findings of this study provided theoretical basis and data reference for PPV7 prevention and control and vaccine research.     

鸭传染性浆膜炎疫苗的研究

用从浙江省分离鉴定的鸭疫里氏杆菌RA-J、RA-L菌株研制疫苗,采用改进的液体培养和鸭胚培养工艺,抗原含量分别达120-186亿CFU／ml和280-310CFU／ml,免疫0.5ml/只,2MLD强毒攻击,平均保护率达94.2％（48／52）,免疫后第7天保护率达80％（16／20）,10天达93.8％（15／16）,疫苗在4℃-8℃条件下保存7个半月和1年的保护率达90％和85％。野外扩大试验和临床推广试用236万羽份表明,效果良好。     

新建茶园不同间作模式及覆盖遮阴效应比较研究——种植模式Ⅰ:茶行东西走向

研究了在东西走向的茶行中分别间作玉米、大豆、玉米+大豆以及覆盖遮阴对新建茶园生态环境、茶籽萌发、茶苗生长以及光合作用的影响。结果表明:⑴茶行东西走向以间作大豆的效果最好。间作大豆的茶园温度可降低3.1～3.6℃,光照强度降低86.4%～90.4%,日均相对湿度增加10.6%,土壤含水量增加13.0%,日均光合速率为对照的2.7倍,茶苗成活率、株高、茎粗、单株叶面积分别增加32.3%、60.5%、33.1%2、18.9%。⑵与覆盖遮阴相比,间作更能提高茶园的空气湿度。