Mutational robustness of ribosomal protein genes

The distribution of fitness effects (DFE) of mutations is of fundamental importance for understanding evolutionary dynamics and complex diseases and for conserving threatened species. DFEs estimated from DNA sequences have rarely been subject to direct experimental tests. We used a bacterial system in which the fitness effects of a large number of defined single mutations in two ribosomal proteins were measured with high sensitivity. The obtained DFE appears to be unimodal, where most mutations (120 out of 126) are weakly deleterious and the remaining ones are potentially neutral. The DFEs for synonymous and nonsynonymous substitutions are similar, suggesting that in some genes, strong fitness constraints are present at the level of the messenger RNA.     

Dose–response relationships between wood moisture content,wood temperature and fungal decay determined for 23 European field test sites

Scots pine sapwood (Pinus sylvestris L.) and Douglas fir heartwood (Pseudotsuga menziesii Franco) specimens were exposed in double layer field trials at 23 different European test sites under different exposure conditions (in total 27 test sets). The material climate in terms of wood moisture content (MC) and wood temperature was automatically monitored over a period of up to 7 years and compared with the progress of decay. The overall aim of this study was to establish dose–response relationships between climate factors and decay as a basis for the service life prediction of wood. The “Scheffer Climate Index” based on weather data collected at official meteorological stations at the different test sites poorly correlated with the corresponding decay progress and was therefore not a suitable tool for estimating site-specific decay potential. In contrast, the use of the combined material climatic parameters MC and wood temperature led to a feasible dose–response function and turned out to be a useful basis for service life prediction of wood.     

Floral acoustics: conspicuous echoes of a dish-shaped leaf attract bat pollinators

The visual splendor of many diurnal flowers serves to attract visually guided pollinators such as bees and birds, but it remains to be seen whether bat-pollinated flowers have evolved analogous echo-acoustic signals to lure their echolocating pollinators. Here, we demonstrate how an unusual dish-shaped leaf displayed above the inflorescences of the vine Marcgravia evenia attracts bat pollinators. Specifically, this leaf's echoes fulfilled requirements for an effective beacon, that is, they were strong, multidirectional, and had a recognizable invariant echo signature. In behavioral experiments, presence of the leaves halved foraging time for flower-visiting bats.     

Influence of treatment temperature and duration on selected biological, mechanical, physical and optical properties of thermally modified timber

The impact of heat-treatment temperature (180, 200, 210, 220 and 240°C) and various heat-treatment durations on selected biological, mechanical, optical and physical properties of thermally modified timber (TMT) was determined. The suitability of different measures for prediction of the treatment intensity was also investigated. Resistance to impact milling (RIM), lightness L*, equilibrium moisture content (EMC), and antiswelling efficiency (ASE) were correlated with corresponding fungal resistance achieved by heat treatments. The results show that the decrease in mass by heat treatments is a suitable measurand to describe the treatment intensity, which is a product of treatment temperature and duration, where the impact of temperature is dominant over the impact of time. The properties examined showed a strong reciprocally proportional relationship with the decrease in mass. Different correlations were found for the various treatment temperatures: the higher the temperature applied, the lower the decrease in mass required for an equivalent improvement in certain wood properties, e.g. biological durability, EMC and dimensional stability. However, mass loss by Poria placenta correlated well with RIM, lightness L*, EMC and ASE of the different heat-treated specimens, depending on the heat-treatment temperature. Consequently, a reliable estimation of improved fungal resistance of TMT, as well as quality control of TMT in general, requires certain process information.     

Kinetics of cell formation and growth stresses in the secondary xylem of Swietenia mahagoni (L.) Jacq. and Khaya ivorensis A. Chev. (Meliaceae)

The kinetics of xylem formation in four-year-old plants of Swietenia mahagoni (L.) Jacq. and Khaya ivorensis A. Chev. were studied by means of high resolution laser measurements (accuracy: ±2 μm) in a spatial resolution of 18.7 to 94.1 μm and a temporal resolution of 1 to 60 s. The radial enlargement of the xylem cells was completed within 0.36 to 8.85 hours in Swietenia and within 0.52 to 12.03 hours in Khaya, while secondary wall formation and lignification lasted days to weeks. Cell enlargement of vessels and ray parenchyma was significantly faster than radial enlargement of fibres and axial parenchyma. The processes of formation of the secondary cell wall and the lignification were slower in fibres than in vessels and parenchyma cells. In Swietenia new secondary cell formation was induced in distinct growth periods almost simultaneously in the whole shoot, while in Khaya new cell formation was restricted to parts of the shoot. Growth stresses in the shoot were studied in terms of released strain. Higher growth stresses were observed in the shoot of Khaya compared to Swietenia. The results indicate that the higher growth stresses in the xylem of Khaya compared to Swietenia originate in the different kinetics of cell development of different cell types in combination with the heterogenous sequence of cell formation within the shoot. Dedicated to Prof. Dr. Dr. h.c. mult. W. Liese on the occasion of his 80th birthday.     

Study of nebulization delivery of aerosolized fluorescent microspheres to the avian respiratory tract

This study investigated the delivery of an aerosol of monodisperse microspheres to the respiratory tract of birds following aerosol exposure. Adult domestic pigeons (Columbia livia domestica, n = 5 birds per timed treatment) were exposed to an aerosol of fluorescent 1.0 microm diameter carboxylate microspheres for 0.5, 1, 2, or 4 hr. During the aerosolization period, the birds were free-standing in a plexiglass treatment chamber and the aerosol was delivered using a commercial nebulizer. Immediately following aerosol exposure, the birds were euthanatized and the carcasses were intravenously infused with a modified paraformaldehyde/gluteraldehyde fixative. Evaluation of microsphere distribution was performed using a stereoscopic microscope with an epifluorescent module. The results from this study revealed that the amount of aerosolized particles delivered using a commercial nebulizer was proportional to exposure periods. Aerosol exposure periods of 0.5 hr or 1 hr did not result in a readily observable distribution of 1.0 microm fluorescent microspheres to the cranial thoracic, caudal thoracic, or abdominal air sac membranes. This was partly attributed to the relatively low concentration of the individual monodisperse microspheres in the aerosolized suspension. The 2- and 4-hr exposure periods resulted in readily observable deposition of the 1.0 mirom fluorescent microspheres in the cranial thoracic, caudal thoracic, or abdominal air sac membranes, with the 4-hr exposure period resulting in the greatest number of particles on the membrane surfaces. For each of the exposure periods, there was individual animal variation regarding the distribution and relative number of spheres deposited. This study demonstrates the widespread deposition of particles that had an aerodynamic equivalent diameter of approximately 1 microm and provides a better understanding of particle deposition efficiency within the respiratory system following aerosol exposure in birds.     

Long-term adaptation potential of Central European mountain forests to climate change: a GIS-assisted sensitivity assessment

An ecological risk assessment is described for determining the adaptation potential of the approximately 11 000 Swiss Forest Inventory points (FIP) to a hypothetically changing climate. The core of the study is a spatially explicit forest community model that generates estimates of the potential natural vegetation for the entire potential forest area of Switzerland under today's as well as under altered climate regimes. The model is based on the Bayes formula. The probabilities of the communities occurring along ecological gradients are derived from empirical data featuring the relationships between quasi-natural vegetation types and measured site variables. Bioclimatological input variables are the quotient between July temperature and annual precipitation (model version A) or mean annual temperature (model version B). Other site variables include aspect, acidity of top soil and, to account for continentality, geographical region. Climate change scenarios are defined as follows: ‘Moderate climate change’ implies an increase of the mean annual temperature of 4°C to 1.4°C depending on the region (model version B) or an increase of the July temperature of 1.5°C (model version A). ‘Strong climate change’ implies an increase of the mean annual temperature of 2°C to 2.8°C (model version B) or an increase of the July temperature of 3.0°C (model version A).

The simulation experiment showed that the geographical distribution of 15 potential natural forest types (distinguished on the basis of floristic affinities) varies considerably with changing temperature. Under moderate warming 30–55% of the FIP change their potential natural vegetation type, whereas under strong climate change the values increase to 55–89% depending on the model version used. In the ecological risk assessment the existing tree species composition on any FIP was compared with the expected tree species composition under today's as well as under altered climate regimes. A major finding indicated that, under the current climate conditions, approximately 25–30% (depending on the model version used) of all FIP must be considered as poorly adapted, i.e. less than 20% of the actual basal area consists of tree species that are expected as dominating taxa. This definition applies for trees with a diameter at breast height (DBH) ≥ 12 cm. Moderate warming increases the percentage of poorly adapted FIP by 5–10% (relative to all FIP considered), strong warming leads to a 10–30% increase of poorly adapted FIP (relative to all FIP considered). If trees with a DBH < 12cm are considered, the percentage of FIP that have to be classified as poorly adapted is reduced significantly. There are strong regional differences as exhibited in risk maps of 10 km × 10 km resolution.     

An orf-like condition caused by trombiculid mites on sheep in South Africa.

Some flocks of sheep in the Amersfoort district of Transvaal Province developed orf-like lesions, commencing between December and April. The causative agent was identified as a new species of mite belonging to the genus Guntheria of the family Trombiculidae.     

POST-OPERATIVE COMPUTED TOMOGRAPHY IN TWO DOGS WITH CEREBRAL MENINGIOMA

Post-operative computed tomography (CT) has been described as a technique for diagnosing incomplete resection or recurrence of cerebral neoplasms in humans. The characteristics of immediate postoperative CT images in dogs with intracranial pathology are unknown. This report describes findings from preoperative, immediate post-operative, and 4 week to 9 month follow-up CT examinations in two dogs with histologically-confirmed cerebral meningiomas. In images of one dog after surgery there was mild contrast enhancement of the tissue surrounding the surgical site. This enhancement had resolved in later images and was probably the result of surgically induced trauma. In post operative images of the other dog there was significant hyperattenuation of the tissues around the surgical site. In post contrast images there was increased enhancement that was evident in later images. These findings, although not supported by necropsy, probably indicate incomplete excision of the tumor.     

Genetic connectivity and phylogeography of the night shark (Carcharhinus signatus) in the western Atlantic Ocean: Implications for conservation management

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