Progress on role of CCN1 in pulmonary diseases

Cysteine-rich angiogenic inducer 61 (Cyr61/CCN1) is an extracellular matrix-associated signaling protein consisting of 381 amino-acid residues, which has the regulatory function for a multitude of cellular responses. The pleiotropic effects of CCN1 on the initiation and resolution of inflammation as well as oncogenesis and development of tumor were reported. According to the numerous data from experimental and clinical studies, this article provides an overview on CCN1 and summarizes the latest understanding of the role of CCN1 in pulmonary diseases.     

泡泡树不同品种果实性状差异及生长动态分析

对引种的泡泡树4个品种果实单果重、纵径、横径、果形指数及生长动态进行了初步研究，结果表明不同品种间单株结果量存在较大差异。根据当前单株结果量和果实性状表现，筛选出Mary Foos Johnson和Overleese两个早期丰产的优良品种；同时发现各品种之间果实横径和纵径大小存在差异。4个泡泡树品种的纵横径变化在泡泡果实成熟前期都随着时间的增加而增大。     

Study on animal intracorneal implantation model with xenogeneic swine corneal stroma

AIM: To study the immunogenicity and biocompatibility of xenogeneic swine corneal stroma as biological carrier for cornea reconstruction and to reconstruct corneal endothelial tissue with this carrier in vitro. METHODS: (1) The lymphocytes from the peripheral blood of F344 rats were immunologically labeled by anti-rat CD25-FITC and anti-rat CD4/CD8-PE, then determined by flow cytometry (FCM) at 12th， 90th day after intracorneal implantation with fresh and dehydrated swine corneal stroma. (2) The fresh and dehydrated grafts made of swine corneal stroma were implanted intralamellarly in corneas of New Zealand rabbits. Clinical examinations were performed monthly and histological examinations were made at 14th， 30th， 60th， 120th and 240th day. (3) The cat corneal endothelial cells were seeded on the Descemet's membrane of the dehydrated swine corneal stroma, then cultured in the medium with epidermal growth factor and laminin for 7 days. The morphology of reconstructed tissue was tested by microscope. RESULTS: (1) Compared to isograft group and negative control, the expression CD4＋CD25＋， CD8＋CD25＋ and CD4＋/CD8＋ of xenograft rat group implantation with swine corneal stroma did not appear significantly different in statistics (P>0.05). (2) In the total 12 rabbits, all the cornea grafts survived without rejection reaction， corneal haze or corneal neovascularization. The fresh grafts got transparent after 2 months, and the dehydrated grafts got transparent after 6 months. Histological study demonstrated both fresh and dehydrated stroma grafts had fused with rabbits'corneal stroma very well without lymphocytes infiltrating. (3) As shown in histological observations, the reconstructed cat corneal endothelial tissue was similar to the nature tissue. Cultured cat endothelial cells connected tightly to each other and attached to the Descemet's membrane closely. CONCLUSION: Swine corneal stroma has low immunogenicity and satisfying biocompatibility,it is an ideal biological carrier for cornea reconstruction in vitro.     

乳源性生长因子对仔猪胃肠道生长发育的影响

猪初乳中含有多种生长因子,包括胰岛素样生长因子、胰岛素和表皮生长因子等。这些乳源性生长因子在促进新生仔猪胃肠道生长发育以及提高损伤黏膜修复等方面起着重要的调节作用。     

断奶日龄对仔猪心肌、背最长肌和半腱肌抗氧化功能的影响

为研究不同断奶日龄对仔猪心肌、背最长肌和半腱肌抗氧化功能的影响,试验选取受试仔猪24头,随机分为4个处理,每个处理6个重复,每个重复1头仔猪,各处理仔猪分别在14日龄、21日龄、28日龄和35日龄断奶,所有仔猪在42日龄宰杀取样.测定仔猪相应组织的MDA含量和抗氧化酶活性.结果表明,随着断奶日龄的推迟,仔猪心肌、背最长肌和半腱肌中MDA含量逐渐降低,35日龄断奶组仔猪心肌MDA含量显著低于14日龄组（P＜0.05）,28日龄和35日龄断奶组背最长肌和半腱肌MDA含量显著低于14日龄组（P＜0.05）;与28日龄和35日龄断奶组相比,14日龄断奶组心肌T-SOD、GSH-Px活性（P＜0.05）以及半腱肌GSH-Px活性均显著降低（P＜0.05）,各断奶日龄组仔猪背最长肌抗氧化酶活性无显著差异.以上结果提示,仔猪断奶日龄越早,其肌肉组织脂质过氧化反应越强,抗氧化功能越低.     

中药组方对黄曲霉毒素B1中毒雏鸭肝功能的影响

为观察黄曲霉毒素B1(AFB1)对试验雏鸭肝功能血清指标的影响及复方中药对AFB1的颉颃效应,本试验选用7日龄健康雏鸭90只,分为3组,每组30只。Ⅰ组为空白对照组,灌胃与试验组等体积二甲基亚砜;Ⅱ组、Ⅲ组为试验组。每天分别按0.1mg/kg剂量给Ⅱ组、Ⅲ组雏鸭灌胃AFB1一次,连续投药21d,试验期间给Ⅲ组雏鸭日粮中添加2%复方中药。分别在给雏鸭投药后7、14、21d,检测雏鸭肝功能部分血清指标。结果显示,Ⅱ组、Ⅲ组雏鸭丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)与Y-谷氨酰转肽酶(GGT)活性显著高于Ⅰ组(P<0.05),而血清总蛋白(TP)与白蛋白(ALB)含量显著下降(P<0.05);与Ⅱ组比较,在日粮中添加复方中药的Ⅲ组雏鸭各项血清指标均有显著改善(P<0.05)。说明黄曲霉毒素B1导致雏鸭肝功能发生显著的变化,而复方中药能明显改善其变化。     

云南省玉溪市玉米致死性坏死病毒原的分子鉴定

玉米致死性坏死病是由玉米褪绿斑驳病毒(Maize chlorotic mottle virus,MCMV)和一种或多种马铃薯Y病毒科病毒复合侵染引起的。2014年1月在对云南省玉溪市玉米病毒病害的调查中发现了一些表现严重花叶、矮化叶片甚至整个植株坏死症状的玉米。对采集样品进行RT-PCR检测,所有样品中都同时检测到了MCMV和甘蔗花叶病毒(Sugarcane mosaic virus,SCMV),在一个样品中同时检测到了MCMV、SCMV和南方水稻黑条矮缩病毒(Southern rice black-streaked dwarf virus)。     

山西省的绿篱植物资源与利用

对山西省现有23种主要绿篱植物，分别从形态、习性、繁殖、利用等方面作了介绍，并就一般绿篱的分类，及通用的栽培方法作了简要介绍，以便为绿篱植物资源的开发利用和城乡环境的绿化、美化提供依据。     

Safe conservation and utilization of peanut germplasm resources in the Oil Crops Middle-term Genebank of China

Peanut(Arachis hypogaea L.)is an important oil and cash crop in the world.Peanut germplasm collected in China are abundant,which provides important material gua...     

天然海藻肥对“夏黑”葡萄植株生长及果实品质的影响

采用单因素试验,以清水为对照(CK)、海藻肥不同稀释倍数(900、1200、1500、1800、2 100倍液)叶面喷施“夏黑”葡萄,研究其对植株生长及果实品质的影响。结果表明:以 1 500倍液的海藻肥处理对“夏黑”葡萄植株生长影响显著,其叶宽、叶长、叶柄长、新梢粗度、新梢节间长度与其它各处理间比较增长较快;葡萄果实的单粒横纵直径、糖度、可溶性固形物等品质要素指标增加显著;葡萄的单粒重、单穗重、单株产量增加显著,每 667 m 2产量增加 15%左右。