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191.
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C. Frömbling 《Forstwissenschaftliches Centralblatt》1913,35(6):296-303
Ohne Zusammenfassung 相似文献
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C.?Silvar J.?M.?Duncan D.?E.?L.?Cooke N.?A.?Williams J.?Díaz F.?MerinoEmail author 《European journal of plant pathology / European Foundation for Plant Pathology》2005,112(1):43-52
A PCR-based method was developed for the identification and detection of Phytophthora capsici in pepper plants. Three PCR primers (CAPFW, CAPRV1 and CAPRV2) specific for P. capsiciwere designed based on the sequence of its internal transcribed spacer regions. CAPFW/CAPRV1 amplify a 452 bp product from P. capsici DNA whereas CAPFW/CAPRV2 a 595 bp fragment; neither set amplifies DNA from pepper or several fungi pathogenic to pepper. In conventional (single-round) PCR, the limit of detection was 5 pg DNA for both primer sets, whereas in nested PCR the detection limit for both was of 0.5 fg. However, when the dilution series of target DNA were spiked with plant DNA, amplification declined two-fold in both conventional and nested PCR. The CAPFW/CAPRV2 set in conventional PCR was used to detect P. capsici DNA in inoculated plants. Detection occurred as soon as 8h post-inoculation in stem samples from infected but still symptomless plants. The method was also tested to detect fungal DNA in infected soils. 相似文献
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An African Swine Fever virus (ASFV) isolated in an 1983 outbreak of the disease in Piemonte, Italy, was related by restriction endonuclease analysis of the viral genome to ASFV strains isolated in the Dominican Republic (1978), Haiti (1981) and Cameroon (1982). 相似文献
198.
A comparison of three rapid diagnostic methods for the detection of rotavirus infection in calves 总被引:1,自引:0,他引:1
S Edwards D Chasey P Napthine J Banks C Hewitt-Taylor M P Cranage 《Veterinary microbiology》1987,13(1):19-25
Three techniques for the detection of rotavirus in faecal samples from calves with neonatal gastroenteritis were compared. A preliminary study indicated that reverse passive haemagglutination (RPHA) was at least as sensitive as the enzyme-linked immunosorbent assay (ELISA). These two immunoassays were compared with the detection of viral RNA by polyacrylamide gel electrophoresis (PAGE) on 209 field samples. Of the 77 samples in which at least one test gave a positive result, 69 were positive by both RPHA and PAGE, but only 49 were also positive by ELISA, indicating a lower sensitivity for the latter test. The overall agreement between RPHA and PAGE was 96%. The reasons for the discrepancies between the tests are discussed. 相似文献
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Characterisation of the outer membrane protein antigens of British field isolates of Moraxella bovis 总被引:1,自引:0,他引:1
Outer membranes of a single isolate of Moraxella bovis were prepared and their purity evaluated by a comparison of the iodinated proteins from whole cells and on outer membranes. The protein patterns of the outer membranes of 10 isolates were examined by SDS-polyacrylamide gel electrophoresis, and the antigenic relationship between proteins of different isolates determined by immunoblotting, using antiserum produced against the outer membrane of the single isolate of M. bovis. The overall protein pattern of the different isolates was similar although not identical, but, significantly, only three separate immunogenic proteins were common to all the isolates under examination. 相似文献