The objective of this study was to create various pH/temp decline rates in hot‐boned bull beef M. longissimus lumborum (LL) through a combination of electrical stimulation (ES) and pre‐rigor holding temperature. The relationship between the pre‐rigor interventions, the activities of µ‐calpain and small heat shock proteins (sHSP), and the impacts on meat product quality were determined. Paired LL loins from 13 bulls were hot‐boned within 40 min of slaughter, immediately ES and subjected to various holding temperatures (5, 15, 25, and 35°C) for 3 hr. The rate of muscle pH decline, sarcomere length, shear force, and proteolysis of muscle proteins were measured. ES‐25°C had a longer sarcomere length compared to non‐electrical stimulation samples. ES‐25°C and ES‐35°C samples had lower shear force values, higher µ‐calpain activity and higher desmin, troponin‐T, and sHSP degradation. The above findings suggest that pH/temp decline rates created in hot‐boned muscle impacted muscle protein proteolysis by increasing the activity of proteases and degradation of sHSP. 相似文献
The variability in fruit maturity and quality has difficulties in determining the optimal harvesting maturity. Therefore, maturity stage in harvest is crucial for both fruit quality and minimizing the fruit detachment force. This study investigates the fruit characteristics and detachment parameters of sour cherry (cv. ‘Kütahya’) in terms of different maturity stages. Sour cherries were grouped into five different maturity stages by observation according to their color and dimensions. The fruit mass and dimension increased as a function of maturity stage; however, the fruit mass remained almost same after the 4th stage. The highest values of total soluble solids and titrable acid contents of sour cherry were gained in the last stage of maturity. The value of chroma of fruit outer color was higher 50?% than that of fruit inner color in the 1st maturity stage. However, it was almost same in the 5th stage of maturity. Increasing fruit maturity caused a decrease in the values of fruit detachment force ranging from 4.25 to 1.78 N, giving the coefficient of determination of 0.947. Similarly, the ratio of fruit detachment force to fruit mass, detachment stress, and specific energy decreased as the fruit maturity increased. The harvest of sour cherry is done only once in the optimum time to decrease the harvest cost. Minimizing the variability in fruit maturity throughout the tree is essential for gaining the maximum profit and lower fruit detachment force in this convenient time. Results of this study indicated that 3rd?5th maturity stages were critical for harvest. In order to attain the maximum poundage per tree and color for the market the most of the fruits should be in these stages. Therefore, appropriate management strategies that could homogenize the maturity throughout the tree should be applied. 相似文献
The Anatolian ground squirrel (Spermophilus xanthoprymnus) is a typical example of true mammalian hibernators. In order to adapt to extreme external and internal environments during hibernation, they lower their body temperatures, heart rates and oxygen consumption; however, pathological events such as ischemia and ventricular fibrillation do not occur in their cardiovascular systems. During the hibernation, maintenance of cardiac function is very important for survival of ground squirrels. Natriuretic peptides (NPs) are key factors in the regulation of cardiovascular hemostasis. Since NPs’ role on the protection of heart during hibernation are less clear, the aim of this study was to investigate dynamic changes in NPs content in the cardiac chambers and to reveal the possible role of NPs on establishing cardiac function in ground squirrel during hibernation using immunohistochemistry. The immunohistochemical results indicate that cardiac NP expressions in atrial and ventricular cardiomyocytes were different from each other and were sex-independent. ANP and BNP were expressed in a chamber-dependent manner in female and male squirrel hearts. Furthermore, cardiac NPs expression levels in hibernation period were lower than those at the pre-hibernation period. During prehibernation period, ANP, BNP and CNP were expressed in the white and beige adipocytes of epicardial adipose tissue (EAT); while during hibernation period, the brown adipocytes of EAT were positive for BNP and CNP. These data suggest that the hibernation-dependent reduction in levels of NPs, particularly ANP, in cardiac chambers and EAT may be associated with low heart rate and oxygen consumption during hibernation. However, further studies are needed to better delineate the roles of NPs during the hibernation.
A study was conducted to determine the effects of feeding spineless cactus cladodes on diuresis and urinary electrolyte excretion in goats. Five bucks were used in a 5 × 5 Latin square experiment with 17-day periods. Experimental diets contained (g/kg dry matter (DM) basis) 370, 470, 570, 670, and 770 spineless cactus cladodes. Water consumption from feed and urine output increased linearly (P < 0.05) as the level of cactus cladodes in the diet increased. However, water intake from drinking was low and unaffected by cactus cladode level. Creatinine clearance and urinary Na excretion were similar for all dietary treatments while K excretion decrease linearly (P < 0.05) as the level of cactus cladodes in the diet increased. Feeding cactus cladodes caused diuresis and reduced urinary K excretion in goats. Possible reasons for these effects include water over-consumption from cactus cladodes and high dietary K intake. 相似文献
1. This study was conducted to determine the effects of feeding an extruded flaxseed (EF) on layer performance, apparent total tract nutrient retention (ATTR) and egg yolk fatty acid concentrations.
2. Seventy-two White Leghorn laying hens (58-week-old; three per cage) were randomly allotted to one of four dietary treatments: 0%, 7.5%, 15.0% and 22.5% of EF-supplemented diets for 8 weeks.
3. Supplementation with EF had no effect on feed intake, egg production, feed conversion ratio and egg weight. Egg components (yolk, albumen and shell percentages) were similar among treatments, except that shell percentage was greater for layers fed 22.5% EF than those fed 7.5% and 15% EF. The ATTR of dry matter and organic matter were highest for 0% and 7.5% EF, intermediate for 15% EF and lowest for 22.5% EF. Similar reductions on ATTR of crude protein and nitrogen-corrected apparent metabolisable energy were observed for layers fed 22.5% EF relative to those fed 0% or 7.5% EF.
4. Feeding EF at 7.5%, 15.0% and 22.5% of the diet markedly increased (by 92%, 198% and 271%, respectively) egg yolk concentrations of n-3 polyunsaturated fatty acid (PUFA) and reduced saturated fatty acid and n-6 PUFA concentrations.
5. It was concluded that omega-3 labelled eggs (300 mg/60 g of egg) may be produced with low (7.5% of diet) levels of dietary EF without compromising egg production parameters. However, feeding moderate to high levels of EF (i.e. 15% and 22.5% EF) may reduce total tract nutrient and energy utilisation. 相似文献
In the spring of 1998, an outbreak of the cedar processionary moth (CPM) [Traumatocampa ispartaensis (Doanlar and Avcí 2001)], a notodontid moth, began on 400 ha in a 75-year-old stand of Lebanon cedar (Cedrus libani A. Rich.) trees in the western Mediterranean region of Turkey. This was the first monitored outbreak of CPM in the Isparta region. Tree crowns recovered to near normal condition by the middle of each growing season (beginning of July) during the outbreak. From 1999–2003, CPM larvae were present in the same stands and again caused defoliation. The objective of this research was to explore dendrochronology as a means of determining the long-term role of CPM in tree growth and to quantify the historic importance of CPM. In 2003, increment cores were collected from 28 host and 10 nonhost dominant or codominant trees, and annual radial-growth indices from 1947–2003 were calculated for one nonhost and four host sample plots. Cedar tree-ring chronologies were analyzed for evidence of CPM. Tree-ring chronologies from nonhost cedar (undefoliated sample trees) were used to estimate potential growth in the host cedar (defoliated sample trees) during current and past outbreaks. The trees selected as host and nonhost were the same subspecies and varieties. We identified regional outbreaks of CPM by synchronous and sustained growth periods of the trees. Growth functions were defined as the cumulative sum of radial increments. Tree-ring evidence suggests that a large-scale outbreak began in 1954 (lasting from 1954–1961) and small outbreaks began in 1947 (1947–1951), 1985 (1985–1988), and 1998 (1998–2003) in the study area. The average reductions in diameter growth for the periods around 1947, 1954, 1985, and 1998 were 40, 28, 17, and 10% of potential, respectively. We concluded that a narrow latewood band is a significant historic indicator of defoliation by CPM, and the outbreaks appear to be associated with dry winter and spring weather prior to the fall and winter in which feeding occurred. 相似文献
BACKGROUND: The aim of this study was to investigate the prevalence of hepatitis B virus (HBV) genotypes in Isparta, Southwest of Turkey, as well as the clinical features and transmission route for patients with HBV infections. METHODS: Patients (n = 135) with HBV infection were included in the study. Epidemiological and clinical data were obtained. HBV genotypes were determined with a preS2 epitope ELISA kit. RESULTS: Although the HBV transmission route remained unidentified in 51.1% of the patients, blood contact was determined as the most common probable transmission route (38.5%). One hundred twenty-four (91.8%) of 135 samples, could be genotyped. One hundred fifteen (85.1%) were genotyped as type D/E, six (4.4%) were genotyped as type A, two (1.4%) were genotyped as type C, and one (0.7%) were genotyped as type F. CONCLUSION: Genotype D/E is determined as the predominant HBV genotype circulating in Isparta, Southwest of Turkey. No relationship between genotypes and disease severity and transmission route has been detected. 相似文献