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31.
Mai H. ElNaggar Ghada M. Abdelwahab Omnia Kutkat Mohamed GabAllah Mohamed A. Ali Mohamed E. A. El-Metwally Ahmed M. Sayed Usama Ramadan Abdelmohsen Ashraf T. Khalil 《Marine drugs》2022,20(3)
Several natural products recovered from a marine-derived Aspergillus niger were tested for their inhibitory activity against SARS CoV-2 in vitro. Aurasperone A (3) was found to inhibit SARS CoV-2 efficiently (IC50 = 12.25 µM) with comparable activity with the positive control remdesivir (IC50 = 10.11 µM). Aurasperone A exerted minimal cytotoxicity on Vero E6 cells (CC50 = 32.36 mM, SI = 2641.5) and it was found to be much safer than remdesivir (CC50 = 415.22 µM, SI = 41.07). To putatively highlight its molecular target, aurasperone A was subjected to molecular docking against several key-viral protein targets followed by a series of molecular dynamics-based in silico experiments that suggested Mpro to be its primary viral protein target. More potent anti-SARS CoV-2 Mpro inhibitors can be developed according to our findings presented in the present investigation. 相似文献
32.
Didik T Subekti Fitrine Ekawasti Muhammad Ibrahim Desem Zul Azmi 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(6)
Toxoplasma gondii consists of three genotypes, namely genotype I, II and III. Based on its virulence, T. gondii can be divided into virulent and avirulent strains. This study intends to evaluate an alternative method for predicting T. gondii virulence using hierarchical cluster analysis based on complete coding sequences (CDS) of sag1, gra7 and rop18 genes. Dendrogram was constructed using UPGMA with a Kimura 80 nucleotide distance measurement. The results showed that the prediction errors of T. gondii virulence using sag1, gra7 and rop18 were 7.41%, 6.89% and 9.1%, respectively. Analysis based on CDS of gra7 and rop18 was able to differentiate avirulent strains into genotypes II and III, whereas sag1 failed to differentiate. 相似文献
33.
A crossbreeding experiment was carried out to determine the sire breed effects on fattening performance of fat-tailed lambs.
Rams from four Iranian fat-tailed sheep breeds, namely Kurdi (K), Afshari (A), Chaal (C) and Sanjabi (S) were mated to Kurdi
ewes producing 454 progeny of four genotype groups (KK, AK, CK and SK). A total of 80 lambs comprising ten female and ten
male lambs from each genotype group were used in a fattening experiment during a period of 88 days. Genotype of the lamb had
significant effect on average dry matter intake (ADMI) (P < 0.05). SK lambs had the lowest ADMI. Average daily gain (ADG) and food conversion ratio (FCR) were not influenced by genotype
of the lamb. Sex of the lamb had significant effect on both ADG and FCR (P < 0.01). Slaughter weight (SLW), hot carcass weight (HCW), cold carcass weight (CCW) and daily carcass gain (DCG) were not
affected by lamb's genotype. However, genotype had significant effect on dressing percentage, carcass loss percentage (P < 0.01), carcass length (CL), leg circumference (LC), carcass compactness (CC) (P < 0.01) and carcass density (CD) (P < 0.05). Carcass width (CW) and leg length (LL) were not affected by lamb's genotype. Sex of the lamb had significant effects
on CL, CW, LC, CC, CD (P < 0.01) and LL (P < 0.05). The results indicated the existence of sire breed difference for daily dry matter intake and carcass conformation
in the fat-tailed sheep. 相似文献
34.
Mahr-un-Nisa Shahzad MA Phillips CJ Sarwar M 《Tropical animal health and production》2012,44(5):947-952
Urea is a common ingredient of the diets of intensively fed lambs, but is increasingly required for industrial processes.
Maize steep liquor (MSL) is a by-product of maize grain degradation to produce starch that may be a suitable replacement.
Fifty growing lambs were fed on equinitrogenous diets in which between 0% and 80% of the urea was replaced by MSL; their growth
and metabolism were recorded over 70 days. Increasing replacement of urea by MSL increased feed intake and nutrient digestibilities,
leading to increased growth rates, more efficient feed conversion, and increased nitrogen retention. Concentrations of triiodothyroxin,
thyroxin, glucose, and methionine were increased by replacement of urea by liquor, and plasma urea was reduced. This study
suggests that MSL is a suitable replacement for up to 80% of urea in the diet of rapidly growing lambs. 相似文献
35.
A fusion DNA vaccine co-expressed Eimeria tenella TA4 and chicken IL-2 (chIL-2) was constructed and its efficacy against E. tenella challenge was observed. TA4 gene of E. tenella and chIL-2 gene were cloned into expression vector pcDNA3.1 and pcDNA4.0c in different forms, producing vaccines pcDNA3.1-TA4-IL-2, pcDNA3.1-TA4 and pcDNA4.0c-IL-2. The expression of aim genes in vivo was detected by RT-PCR and western blot. Animal experiment was carried out to evaluate the immune efficacy of the vaccines. Results indicated these DNA vaccines were successfully constructed and the antigen genes could be expressed effectively in vivo. The animal experimental results showed that DNA vaccines could obviously alleviate cecal lesions, body weight loss and increase oocyst decrease ratio. The ACI of pcDNA3.0-TA4-IL-2 group was 192, higher than that of pcDNA3.1-TA4 group. The results suggested that TA4 was an effective candidate antigen for vaccine and co-expression of cytokine with antigen was an alternative method to enhance DNA vaccine immunity. 相似文献
36.
37.
Fernando Machado-Stredel Benedictus Freeman Daniel Jiménez-Garcia Marlon E.Cobos Claudia Nu?ez-Penichet Laura Jiménez Ed Komp Utku Perktas Ali Khalighifar Kate Ingenloff Walter Tapondjou Thilina de Silva Sumudu Fernando Luis Osorio-Olvera A.Townsend Peterson 《中国鸟类》2022,(1):1-10
Studies of biodiversity dynamics have been cast on either long(systematics)or short(ecology)time scales,leaving a gap in coverage for moderate time scales of de... 相似文献
38.
39.
Mehdi Zarifi Sébastien Buczinski Ali Rezakhani Mohammad Reza Mokhber Dezfouli Ali Khonsha 《Journal of Veterinary Cardiology》2012,14(3):415-421
ObjectivesTo study the effect of pregnancy and lactation on echocardiographic parameters in Holstein dairy cows.AnimalsNine multiparous high milk producing (HMP) dairy cows (producing more than 40 kg of milk per day in peak production) and 9 low milk producing (LMP) cows (producing less than 30 kg or milk per day in peak production).MethodsEchocardiography was performed twice; one month before calving and two months after calving.ResultsThe heart rate of HMP cows in the early lactation period was significantly higher than in the dry period. In LMP cows, there was a significant increase in left ventricular dimension in the early lactation period as compared to the dry period, and the interventricular septum in systole (IVSs) in the dry period was significantly thicker than early lactation period. In HMP cows, there was an increase in the right ventricular diameter in systole in the early lactation period as compared to the dry period. Left ventricular and aortic dimensions in the dry period of HMP were significantly higher than those of LMP cows. When the data were corrected for body weight, comparison of values of the dry period of HMP and LMP cows showed that left ventricular volume in systole in HMP was significantly higher and that IVSs, left ventricular fractional shortening and ejection fraction were significantly lower than in LMP cows.ConclusionsThis study demonstrated that lactation influences the intracardiac dimensions. The amount of milk production can influence echocardiographic parameters in dairy cows. 相似文献
40.
BACKGROUND: Entomopathogenic fungi such as Verticillium lecanii (Z.) (Mycotal®) are used for pest control as an alternative to chemical control. In this study, the effect of V. lecanii on cereal aphids is assessed. In addition, an investigation is carried out to determine whether the use of V. lecanii affects the performance of two natural enemies of aphids, the predator Harmonia axyridis (P.) and the parasitoid Aphidius colemani (V.), in no‐choice experiments under laboratory conditions. RESULTS: The number of Rhopalosiphum padi (L.) and Sitobion avenae (F.) killed was increased by increasing the concentration of V. lecanii. The timing of application of fungus to aphids affected the efficacy of other biocontrol agents, a parasitoid and a predator. Parasitation by A. colemani (V.) in both cereal aphids (S. avenae and R. padi) was not affected by V. lecanii when aphids were first treated with V. lecanii and then exposed to A. colemani. The emergence of adults from parasitised mummies was, however, lower in infected aphids than in uninfected aphids when the aphids were first exposed to the parasitoids and then treated with fungus. The female sex ratio in the emerging adults was lower in V. lecanii‐treated aphids in both species. When aphids were first treated with V. lecanii, 72 h before predation, fewer aphids of both species were consumed by H. axyridis (P.). CONCLUSION: Use of entomopathogenic fungus as a biological control agent could be a complementary strategy in an integrated pest management programme against cereal aphids, but it can reduce the efficiency of other biocontrol agents (parasitoids and predators) when applied simultaneously. © 2012 Society of Chemical Industry 相似文献