套袋对‘茌梨’果实蔗糖代谢及相关酶基因表达的影响

 研究了4种材质的果袋套袋处理对‘茌梨’（Pyrus bretschneideri Rehd.‘Chili’）果实生长发育过程中可溶性糖含量及蔗糖代谢的影响。结果表明,果实中的果糖、葡萄糖、蔗糖及可溶性总糖含量均随着果实的发育进程而增加;不同材质果袋影响糖增加的程度不同,套黄蜡纸袋的糖增加最少;转化酶（Ivr）活性随着果实的发育而降低,套袋降低了花后75 ~ 90 d 和花后150 d 果实酸性转化酶（AI）活性,而对中性转化酶（NI）影响不明显;蔗糖磷酸合成酶（SPS）活性随着果实的发育而增加,套袋处理不同程度上降低了SPS活性,以套黄蜡纸袋最为明显,其次是套白蜡纸袋和无纺布袋,套塑料膜袋的大部分测试点与对照差异不显著;随着果实的发育,果实中蔗糖合成酶合成活性（SSs）逐渐增加,蔗糖合成酶分解活性（SSc）逐渐下降,套袋对SSs活性影响在多数测定点差异不显著,但降低了果实发育后期SSc活性。蔗糖代谢相关酶基因AIV1、AIV2、SPS1和SUS1表达的实时荧光定量PCR分析表明,套袋降低了AIV2的相对表达量,降低程度套无纺布袋大于套塑料膜袋;推迟了果实中SPS1的相对表达量最大值出现的时间,套塑料膜袋果的SPS1相对表达量在发育前期显著低于对照;影响了SUS1相对表达量的变化,整个发育过程未见套塑料膜袋果有SUS1相对表达量高峰出现,套黄蜡纸袋果的SUS1相对表达量最大值较对照提前15 d,之后显著低于对照。     

Pancytopenia in a cat with visceral leishmaniasis

Abstract: A 4‐year‐old, domestic shorthair, female spayed cat was presented for decreased appetite and depression. Severe pancytopenia with erythrocyte autoagglutination was found. The cat was seronegative for feline immunodeficiency and leukemia viruses. Immune‐mediated hemolytic anemia was suspected but no response to treatment with a blood transfusion, enrofloxacin, and prednisone was observed. Blood and bone marrow smears obtained 11 days later contained Leishmania amastigotes in the cytoplasm of neutrophils and macrophages, respectively. Serologic and PCR testing of peripheral blood confirmed infection with Leishmania infantum. Despite treatment, the cat worsened clinically and was euthanized. At necropsy, visceral dissemination of the parasite was confirmed. The findings in this case indicate that visceral leishmaniasis should be considered as a differential diagnoses in cats with pancytopenia in areas endemic for Leishmania. In addition, amastigotes may be observed in peripheral blood neutrophils.     

福建将乐林场常绿阔叶次生林主要种群种间联结性研究

【目的】研究福建省将乐国有林场常绿阔叶次生林主要种群间的联结性,为探索植物群落演替及原生性植被类型的恢复提供依据,为该林场常绿阔叶次生林的可持续经营提供参考。【方法】采用样地调查法,在福建将乐林场选取重要值较大的22个树种,利用种间联结指数VR研究群落的整体关联性;利用χ2检验、Pearson相关系数检验、Spearman秩相关系数检验分析种对间的联结性。【结果】该林场常绿阔叶次生林种间联结指数VR为1.014,主要种群总体呈现较弱的正关联。231个种对中,χ2检验结果显示,正、负关联的种对数分别为110和119对,正负关联种对数的比值为0.924;显著和极显著相关种对数占总数的11.3%。Pearson相关系数检验结果表明,正、负相关的种对数分别为77和153对,正负关联种对数的比值为0.503;显著和极显著相关的种对数占总数的7.4%。Spearman秩相关系数检验结果表明,正、负相关的种对数分别为100和129对,正负关联种对数的比值为0.775;显著和极显著相关的种对数占总数的13.9%。【结论】福建将乐林场的常绿阔叶次生林总体联结程度较小,22个主要树种种对间关系以负相关较多,种间关联性不明显,主要种群独立出现的概率较大。     

紫花苜蓿土壤浸提液对蜀葵的化感作用

为探究紫花苜蓿对蜀葵种子萌发和生长的化感作用,采取了紫花苜蓿根际及距离根际20、40、60 cm处表层5~10 cm的土壤,采用高效液相色谱法（HPLC）检测土壤浸提液（分别表示为R、D20、D40、D60）中5种有机酸类化感物质的含量。采用生物检测方法分析测定了土壤浸提液对蜀葵种子萌发的影响,并通过盆栽试验测定了紫花苜蓿与蜀葵间作条件下蜀葵叶绿素、丙二醛（MDA）、可溶性蛋白及生物量等生理生态学指标。结果表明： 5种有机酸在紫花苜蓿根际的含量最高,随着与根际距离的增加有机酸含量逐渐减少;R处理下,蜀葵发芽率和发芽指数比对照（蒸馏水）分别降低6.23%和31.32%,根长和苗长则与对照相比分别显著减少了56.11%和48.94%。盆栽实验结果表明,紫花苜蓿和蜀葵间作对蜀葵地上和地下生物量均产生明显抑制作用,与单种时相比分别减少8.01%和23.99%;间作使蜀葵叶绿素含量降低、MDA和可溶性蛋白含量升高。研究表明,紫花苜蓿对蜀葵有明显的化感作用,种植时不宜过密,应至少保持间距60 cm。     

日粮中添加NCG对妊娠前期鄂尔多斯细毛羊血液、尿囊液和羊水中氨基酸浓度的影响

为了研究日粮中添加NCG(N-氨甲酰谷氨酸)对妊娠前期鄂尔多斯细毛羊血液、尿囊液和羊水中氨基酸浓度的影响,试验选择年龄、胎次和体况相近、平均体重为50.62±0.52 kg的受孕鄂尔多斯细毛羊母羊40只,随机分为3组(NCG1组饲喂基础日粮+0.30 g NCG/d(n=13),NCG2组饲喂基础日粮+0.40 g N...     

外源生长素对烟草髓愈伤组织分化和内源IAA含量的影响

在普通烟草髓愈伤组织的培养过程中,高的外源ＮＡＡ促进内源ＩＡＡ的合成,且有利于根的形成,但与内源生长素的含量不成正比关系．培养基中的ＢＡ和ＮＡＡ含量高皆引起玻璃化,玻璃化与两者的比值无关     

Quantitative trait loci analysis for root traits in synthetic hexaploid wheat under drought stress conditions

Synthetic hexaploid wheat(SHW), possesses numerous genes for drought that can help breeding for drought-tolerant wheat varieties. We evaluated 10 root traits at seedling stage in 111 F9 recombinant inbred lines derived from a F2 population of a SHW line(SHW-L1) and a common wheat line, under normal(NC) and polyethylene glycol-simulated drought stress conditions(DC). We mapped quantitative trait loci(QTLs) for root traits using an enriched high-density genetic map containing 120 370 single nucleotide polymorphisms(SNPs), 733 diversity arrays technology markers(DArT) and 119 simple sequence repeats(SSRs). With four replicates per treatment, we identified 19 QTLs for root traits under NC and DC, and 12 of them could be consistently detected with three or four replicates. Two novel QTLs for root fresh weight and root diameter under NC explained 9 and 15.7% of the phenotypic variation respectively, and six novel QTLs for root fresh weight, the ratio of root water loss, total root surface area, number of root tips, and number of root forks under DC explained 8.5–14% of the phenotypic variation. Here seven of eight novel QTLs could be consistently detected with more than three replicates. Results provide essential information for fine-mapping QTLs related to drought tolerance that will facilitate breeding drought-tolerant wheat cultivars.     

10%硝磺草酮(千层红)防除玉米田苗后杂草试验

掌握10%硝磺草酮对玉米田苗后杂草的防除效果、安全性及使用技术,为大面积推广应用提供科学依据。     

高校图书馆数字资源宣传推介模式优化研究

归纳总结了山东本科院校图书馆数字资源宣传推介的诸多模式,分析了这些宣传模式在实践中存在的主要问题,并提出优化宣传推介模式、提高宣传推介效果的方案及保障措施。     

高密度对甘肃黄灌区玉米品种农艺性状和产量的影响

选择4个玉米品种,设置3种密度,于2015-2016年在甘肃中部沿黄灌区种植试验得出：同一品种在不同密度下的农艺性状和产量存在差异。对宁玉524、辽单588、高玉818、先玉335的各农艺性状和产量构成因素在密度9.75万、11.25万、12.75万株/hm ²处理下两年测得的数据均值进行了分析比较,发现随着种植密度的增大,玉米的农艺性状和产量出现趋向性变化。表现为玉米的空秆率、倒伏率、株高、穗位高和产量随着密度的增加而增加;茎粗、穗粗、穗长、穗粒数和百粒重随密度增加有所减小。其中空秆率、茎粗、穗粗、穗长、穗粒数和产量差异显著,高种植密度对他们有显著的影响。