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991.
992.
993.
Patrizia Erre Innocenza Chessa Concepción Muñoz-Diez Angjelina Belaj Luis Rallo Isabel Trujillo 《Genetic Resources and Crop Evolution》2010,57(1):41-54
The genetic relationships within and between wild and cultivated olives were examined and clarified in an isolated and restricted
area, such as the Mediterranean island of Sardinia. Wild (21 individuals) and cultivated olive trees (22 local cultivars from
a germplasm collection and 35 ancient trees) were genotyped by means of 13 SSR loci. Five cases of synonymy were observed
and nine distinct genotypes were identified in the collection. Five novel genotypes were also detected among the ancient trees.
Differences on the allelic composition and heterozygosity levels were found between wild and cultivated trees. Model-based
clustering method classified the olive trees into two major gene pools: (a) wild genotypes and (b) local cultivars from the
collection and from heritage olives. Regarding the cultivated plant material, we observed that: (a) most of the Sardinian
cultivars shared the same allelic profiles with the ancient cultivated trees and (b) the majority of these cultivars and all
the novel genotypes were not related to any other cultivars included in this study. These findings as well as the detection
of unique alleles and a certain wild genetic background at some cultivars revealed by the Bayesian analysis may indicate their
autochthonous origin. The synonymy cases found between local cultivars and Italian mainland cultivars indicate interchange
of genetic material among these growing areas, suggesting thus a possible allochthonous origin. The information obtained can
assist in the management of an olive collection and sheds some light on the survival of true oleasters and the origin of Sardinian
cultivars. 相似文献
994.
Yang Yang Yuezhi Pan Xun Gong Moutian Fan 《Genetic Resources and Crop Evolution》2010,57(8):1239-1248
Citrus hongheensis is a critically endangered species endemic to the Honghe river region in southeastern Yunnan, China. Its genetic diversity
and differentiation were investigated using Inter-Simple Sequence Repeat (ISSR) markers. One hundred primers were screened,
and a total of 245 loci were amplified from seven natural populations by 13 informative and reliable primers. Of these 245
ISSR loci, 233 were polymorphic and the detected variations revealed a relatively high level of intraspecific genetic diversity.
At the population level, the mean percentage of polymorphic loci (PPB) was 36.50%, while the average expected heterozygosity (He) and Shannon diversity index (Ho) were 0.1327 and 0.1972, respectively. At the species level (across all populations), PPB was 95.10%, while He and Ho were 0.3520 and 0.5195, respectively. A high Gst value (0.6247) indicated that there is significant differentiation among populations, which was confirmed by AMOVA analysis
(Φst = 0.6420). Pairwise genetic identity (I) values among populations ranged from 0.6341 to 0.7675, with a mean of 0.7008. We propose that the high level of genetic
differentiation may be the result of habitat fragmentation and limited gene flow (Nm = 0.1502). For effective in situ conservation and population restoration of C. hongheensis it will be important to maintain historical processes, including high outbreeding rates, sufficient gene flow, and large
effective population sizes. 相似文献
995.
Saowaluck Bua-in Yingyong Paisooksantivatana 《Genetic Resources and Crop Evolution》2010,57(3):405-414
The genetic relatedness among 51 accessions, 14 species of the genus Zingiber and genetic variability of a clonally propagated species, Zingiber montanum (Koenig) Link ex Dietr., from Thailand were studied using random amplified polymorphic DNA (RAPD) profiles. Twenty-nine random
primers gave reproducible amplification banding patterns of 607 polymorphic bands out of 611 scored bands accounting for 99.40%
polymorphism across the genotypes. Jaccard’s coefficient of similarity varied from 0.119 to 0.970, indicative of distant genetic
relatedness among the genotype studied. UPGMA clustering indicated eight distinct clusters of Zingiber, with a high cophenetic correlation (r = 1.00) value. Genetic variability in Z. montanum was exhibited by the collections from six regions of Thailand. High molecular variance (87%) within collection regions of
Z. montanum accessions was displayed by AMOVA and also explained the significant divergence among the sample from six collection regions.
Our results indicate that RAPD technique is useful for detecting the genetic relatedness within and among species of Zingiber and that high diversity exists in the clonally propagated species, Z. montanum. 相似文献
996.
Anna A. Filatenko Karl Hammer Sulaiman Al Khanjari Andreas Buerkert 《Genetic Resources and Crop Evolution》2010,57(8):1135-1139
Due to its geographic position on the northeastern tip of the Arabian Peninsula and its sea trade relationships with Asia,
East Africa and the Middle East, Oman has for millennia been at the cross-roads of inter-regional exchange of cultivated plants.
This is reflected in recent findings of new cultivars of banana (Musa spp.) and wheat (Triticum spp.) in remote oases of the Hajar Mountains in northern Oman. Material collected in 2003 and 2004 contained six new botanical
varieties of wheat which are described here. One of them belongs to the tetraploid T. aethiopicum, the others are hexaploid. 相似文献
997.
Francesco Carimi Francesco Mercati Loredana Abbate Francesco Sunseri 《Genetic Resources and Crop Evolution》2010,57(5):703-719
A total of 82 grapevine genotypes were sampled from several areas of the Italian region of Sicily where vineyards are widely
spread. The grapevines were characterized using six microsatellite markers (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62 and VrZAG79)
to evaluate genetic diversity. Thirty-seven of the 82 cultivars sampled had their names quoted in historical and literary
sources, while 45 cultivars from old vineyards did not have their names reported in ancient literature. According to their
genetic profiles at SSR loci, 70 different cultivars were found, while interesting cases of synonymies (Regina and Moscato
bianco, Alicante and Dolcetta or among different clones of Zibibbo and Catarratto) and cases of homonymy (Frappato and Nerello
Mascalese) were discovered. Several genetic parameters were calculated to assess the efficacy of the loci chosen in this work.
Pairwise genetic distances between all cultivars were calculated. A dendrogram representing the genetic similarities among
cultivars was depicted using the UPGMA method to investigate their relationships, explaining them from a historical point
of view. The cluster distribution of cultivars clearly does not reflect their current geographical distribution, suggesting
successive introductions of cultivars in Sicily from different areas of origin. 相似文献
998.
Solomon Benor Frank R. Blattner Sebsebe Demissew Karl Hammer 《Genetic Resources and Crop Evolution》2010,57(2):293-306
Little information is available regarding the biodiversity and potential use of jute (Corchorus species) in Ethiopian agriculture. The present study summarizes species’ occurrence, use, geographical distribution, ecology,
and ethnobotany of Corchorus species in Ethiopia. An ecogeographical study, conducted in the Amhara, Oromia and Gambella Regional States in 2005 and 2008
resulted in the collection of seven different species with more than 100 accessions. The study revealed low similarity in
species composition between the Regional States, indicating that each region has its own unique set of species. Species numbers
are higher in the north-eastern and south-western than the central part of the country. C. aestuans L. and C. tridens L. are restricted to humid lowlands of south-western part of Ethiopia, growing at elevations up to 490 m, whereas, C. urticifolius Wight et Arn., C. trilocularis L. and C. schimperi Cufod. were collected only in the north-eastern part of the country with elevation ranges of 1,380–2,130 m. The only species
collected at a higher elevation (>2,100 m) was C. schimperi Cufod. Farmers’ perception, indigenous knowledge and folk taxonomy of jute species are better in Gambella than the other
studied regions. Although several Corchorus species are reported from Ethiopia, these species are neither cultivated nor popularly used as leafy vegetables. This is
mainly due to lack of awareness or knowledge about the species use, and abundant distribution of the species that allows gathering
of edible leaves with little expenditure of time, labour and other resources. Our results revealed that some species are threatened,
which necessitates highest priority for jute germplasm conservation in the country. In addition, creating public awareness
about the use of jute species as a cheap source of leafy vegetable will play an important role to diversify food sources,
reduce malnutrition, and contribute to household income generation of the farming community. 相似文献
999.
A collection of seeds from five Acacia species was made in Egypt in 1856. Since then, the seeds have been stored at room temperature in different Swedish museums.
Due to the extreme longevity within the seeds of Acacia and related species, germination tests were performed on the now 151-year old seed. Seeds of two of the five species tested
germinated. The first, Acacia farnesiana (L.) Willd., had two seeds germinate, and Acacia melanoxylon R. Br. ex Ait. f. had one seed germinate. In addition, DNA was extracted from the aged seed and DNA preservation was analyzed.
Four of the tested species displayed well preserved DNA, whereas DNA from Albizia lebbeck (L.) Benth. showed signs of degradation. The 151-year longevity of the Acacia seeds is among the longest of dry-stored seeds reported. Several independent studies now report on extreme survival capacity
for Acacia and related genera suggesting that these genera are suitable for studies on the characteristics of seeds with long storage
performance. The results also demonstrate that herbaria and seed collections stored in museums and institutional depositories
can be alternate sources of plants genetic material and should be given conservation attention. 相似文献
1000.
The characterization of plant genetic resources is the first step towards improving their use. The Spanish Plant Genetic Resources
Centre, which belongs to the National Institute for Agricultural and Food Technology Research (CRF-INIA), conserves accessions
of wild underground vetches collected in Spain. In the present work, 26 underground vetch accessions were characterized in
terms of their seed storage proteins (separated by SDS-PAGE) as a means of assessing the genetic variation of these plants
and their agronomic value. Vicia sativa cv. Vereda was used as control. A total of 54 bands were detected, of which 49 were polymorphic. Fifty eight different electrophoretic
patterns were observed in total. Protein bands were scored in terms of their presence (1) or absence (0) for all the seeds
studied, and two matrices constructed, one with all the bands present in each accession, the other with the different patterns
for each accession. Dendrograms based on the Jaccard similarity index and the UPGMA clustering method were produced from these
matrices, and the degree of genetic variation between and within accessions was calculated. The groups obtained were compared
with the chromosome number for each accession. The results reflect the great diversity of underground vetch seed storage proteins.
The aerial and underground seeds of 16 accessions were then analysed separately. In some cases, the aerial and underground
flowers of the same plant produced different proteins. 相似文献