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411.
Isotani M Ishida N Tominaga M Tamura K Yagihara H Ochi S Kato R Kobayashi T Fujita M Fujino Y Setoguchi A Ono K Washizu T Bonkobara M 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2008,22(4):985-988
BACKGROUND: Imatinib mesylate is a small molecule targeted at dysregulated protein-tyrosine kinase. Mutation of c-kit exon 11, which induces constitutive phosphorylation of KIT, is one of the mechanisms for the development or progression of mast cell tumor (MCT) in dogs. The purpose of this study was to examine the therapeutic potential of imatinib mesylate in canine MCT. HYPOTHESIS: Imatinib mesylate has activity against MCT in dogs, and response to treatment can be correlated to presence of mutation within exon 11 of c-kit. ANIMALS: Twenty-one dogs with MCT with gross tumor burden and median tumor size of 7.2 cm (range, 1.0-25.3 cm) before treatment. METHODS: Tumors were analyzed for mutation of c-kit exon 11. Imatinib mesylate was administered PO to the dogs at a dose of 10 mg/kg daily for 1-9 weeks. RESULTS: Ten of 21 dogs (48%) had some beneficial response to imatinib mesylate treatment within 14 days of treatment initiation. All 5 dogs with a demonstrable c-kit mutation in exon 11 responded to the drug (1 complete remission, 4 partial remission). CONCLUSIONS AND CLINICAL IMPORTANCE: Imatinib mesylate has clinical activity against MCT in dogs. Response could not be predicted based on presence of absence of a mutation in exon 11 of c-kit. 相似文献
412.
Characterization of feline T and B cells 总被引:1,自引:0,他引:1
T Kuramochi M Takeishi T Ishida K Kato M Ishida 《American journal of veterinary research》1987,48(2):183-185
Feline peripheral-blood lymphocyte populations (n = 22) were examined for the following markers: rosette formation with guinea pig erythrocytes (GPE-T cells), rosette formation with human RBC (HRBC-T cells), rosette formation with sheep RBC, mixed rosette formation with GPE-T cells and HRBC-T cells (total T cells), erythrocyte antibody-complement rosettes, and surface immunoglobulin. An average of 28% +/- 7% (range, 16% to 39%) of the feline lymphocytes formed rosettes with GPE-T cells, and 27% +/- 7% (range, 11% to 36%), with HRBC-T cells. An average of 57% +/- 9% (range, 33% to 75%) of the lymphocytes formed mixed rosettes. The erythrocyte antibody-complement rosette-forming cells and surface immunoglobulin-bearing cells were found in peripheral blood lymphocytes (10% +/- 6% and 24% +/- 8%, respectively). The murine monoclonal antibodies OKT 11 and HuLy-m1, specific for a framework determinant of human E-rosette receptor antigens, cross-reacted with feline cell membrane molecules recognizing a bimolecular complex (45,000 to 50,000 daltons) similar to that described in persons. We investigated the distribution of these E-rosette receptor-like antigens on feline lymphocytes. By complement-mediated lymphocytotoxicity, about 30% of the feline lymphocytes expressed the antigens. When lymphocytes were treated with HuLy-m1 antibody, spontaneous rosette formation with HRBC-T cells was significantly inhibited. 相似文献
413.
Production of anti-virus,viroid plants by genetic manipulations 总被引:1,自引:0,他引:1
Ishida I Tukahara M Yoshioka M Ogawa T Kakitani M Toguri T 《Pest management science》2002,58(11):1132-1136
Many pathogenic plant viruses are RNA viruses, which initiate production of double-stranded RNA intermediates when they replicate in host plant cells. Introduction of double-stranded RNA-specific ribonucleases such as the Schizosaccharomyces pombe derived pac I protein and animal cell derived interferon-induced 2',5'-oligoadenylate synthetase (2-5 Aase)/ribonuclease L (RNase L) system into various plants may make plants resistant to various pathogenic viruses and viroids. We have demonstrated that pac I and 2-5 Aase/RNase L transgenic tobacco plants are resistant to various viruses including tobacco mosaic virus, cucumber mosaic virus and potato virus Y. In addition, pac I transgenic potato plants are resistant to potato spindle tuber viroid. Using Agrobacterium-mediated transformation, we have established a transformation system for chrysanthemum plants and have recently developed pac I transgenic chrysanthemum (Dendranthema grandiflora cv Reagan) resistant to chrysanthemum stunt viroid and have grown them in isolated fields for an evaluation of their effects. 相似文献
414.
415.
Shukei Masuma Gunzo Kawamura Nobuhiro Tezuka Masahiko Koiso Kenji Namba 《Fisheries Science》2001,67(2):228-231
SUMMARY: In bluefin tuna culture, a high mortality of juveniles is caused by bumping into the tank and net-pen walls at dawn. This bumping can possibly be attributed to visually disoriented behavior of the fish. To examine this possibility, the authors carried out retinomotor response experiments with juvenile bluefin tuna (50.7–96.8 mm total length, at which they were transferred from a indoor tank to a sea net-pen) and measured ambient light intensity at the culture site at dawn. The light intensity at which the transition from scotopic to photopic vision takes place was 7.52 lx and the time taken by the transition was 15 min. At dawn, the ambient light intensity rapidly increased from scotopic light intensity level and attained photopic light intensity level in 10 min. This incompatibility of the retinal adaptation with the change in the ambient light intensity could cause the visual disorientation of the fish. It is therefore possible that the visually disoriented juveniles cannot control their high power swimming and thus bump into the walls at dawn. 相似文献
416.
Separation of the Taiwanese regular and deep tuna longliners in the Indian Ocean using bigeye tuna catch ratios 总被引:1,自引:0,他引:1
ABSTRACT: Taiwanese longline (LL) fisheries operating in the Indian Ocean usually target albacore tuna (ALB), swordfish (SWO) and yellowfin tuna (YFT) using regular LL. Bigeye tuna (BET), however, is targeted using deep LL. Thus, these two types of LL are considered to be different gears as they target different tuna species. Regular or deep LL fishing is defined by number of hooks per basket (NHB): regular LL if 6 ≤ NHB ≤ 10 and deep LL if 11 ≤ NHB ≤ 20. However, NHB information was available in only some of the recent LL data (1995–1999). This situation had caused problems of biased results in stock analysis in the past. Thus, the objective of our study was to explore an effective method to separate the two types of LL fishing by considering species composition. Some intervals of BET catch ratios were found to be effective in separating the regular and deep LL catches, i.e. 0.0 ≤ BET/(BET + ALB + SWO) ≤ 0.4 and 0.8 ≤ BET/(BET + ALB) ≤ 1.0, respectively. Using these two separators, the LL known data set (1995–1999) (learning data set) was classified. Correct classification occurred in 67.7% of the data, while 23.1% of the data were unclassified (11.9% due to zero catches and 11.2% due to classification into both LL types), and 9.2% were misclassifications. Then, using the methods developed, the LL unknown data set in the historical data (1979–1999) was classified and nominal CPUE values were calculated for four species. The CPUE trends based on this study were likely to be more reliable than those of previous studies. 相似文献
417.
418.
Masahiko Suzuki M. A. Chozin Arihiro Iwasaki Kiyotake Suenaga Hisashi Kato‐Noguchi 《Weed Biology and Management》2019,19(1):3-8
Chinese violet (Asystasia gangetica (L.) T. Anderson) is a perennial invasive weed belonging to Acanthaceae. Leaves of this weed have been suggested to possess phytotoxic activity. However, phytotoxic substances in this weed have not yet been reported. Therefore, the present study investigated phytotoxic activity of Chinese violet extracts and phytotoxic substances. The extracts of Chinese violet leaves inhibited the root and shoot growth of cress, lettuce, alfalfa, barnyard grass, ryegrass, and timothy, where the level of inhibition increased with increasing extract concentrations. Bioassay‐guided separations of the extracts led to isolation of two phytotoxic substances, indole‐3‐carboxaldehyde and (6R,9S)‐3‐oxo‐α‐ionol. Indole‐3‐carboxaldehyde significantly inhibited the root and shoot growth of cress at concentrations ≥100 and 30 μmol L?1, respectively, and concentrations of the substance required for 50% growth inhibition were 210 and 127 μmol L?1 for cress roots and shoots, respectively. The other substance, (6R,9S)‐3‐oxo‐α‐ionol, was reported to have strongly inhibited cress roots and shoots. The present results suggest that Chinese violet contains two phytotoxic substances indole‐3‐carboxaldehyde and (6R,9S)‐3‐oxo‐α‐ionol, and those substances may play an important role in the phytotoxic activity of Chinese violet. 相似文献
419.
Plant growth inhibitory activity and active substances with allelopathic potential of cogongrass (Imperata cylindrica) rhizome 下载免费PDF全文
Masahiko Suzuki Tohru Tominaga Osamu Ohno Arihiro Iwasaki Kiyotake Suenaga Hisashi Kato‐Noguchi 《Weed Biology and Management》2018,18(2):92-98
Cogongrass (Imperata cylindrica [L.] Raeusch.) is a perennial rhizomatous grass that belongs to the Poaceae and is widely distributed in tropical, subtropical and temperate regions over the world. The grass is strongly invasive and is suggested to have allelopathic potential. However, limited information is available on allelopathic substances in cogongrass rhizomes. Therefore, the allelopathic potential and substances in cogongrass rhizomes were investigated. The extracts of cogongrass rhizomes inhibited the root and shoot growth of barnyard grass, ryegrass, timothy, cress, lettuce and alfalfa. After bioassay‐guided separations of the extracts, four growth inhibitory substances, 5‐methoxyflavone, 5,2′‐dimethoxyflavone, methyl caffeate and abscisic acid, were isolated. 5‐Methoxyflavone, 5,2′‐dimethoxyflavone and methyl caffeate significantly inhibited the root and shoot growth of cress at concentrations of ≥0.03–0.3 m mol L?1 and their concentrations required for 50% growth inhibition were 0.079–0.24, 0.23–1.1 and 0.59–0.88 m mol L?1, respectively. The other isolated substance, abscisic acid, has been reported to have strong growth inhibitory activity. Its concentrations required for 50% growth inhibition on cress were 0.31–0.61 μ mol L?1. The present study suggests that cogongrass rhizomes might have allelopathic potential and that those four substances might contribute to that potential. 相似文献