阿魏蘑与杏鲍菇高产优质栽培模式研究

试验证明，阿魏蘑和杏鲍菇最佳栽培模式为菌棒半脱袋竖向畦栽半覆土栽培模式；最适环境条件为菇房温度13～20℃，空气相对湿度85％～95％；最佳栽培设施为半地下冬暖式塑料大棚。     

萝卜离体再生的影响因素

对影响萝卜离体再生的因素进行了研究,结果表明,不同基困型材料均以带柄子叶的再生频率最高;不同基因型材料所需的最适激素浓度和配比不同,在一定范围内提高6-BA浓度可促进不定芽的分化;苯基脲类细胞分裂素TDZ与嘌呤型细胞分裂素6-BA和KT相比,对促进萝卜不定芽分化的效果更好;AgNO_3可显著提高不定芽再生频率;NAA诱导生根的效果优于IAA。     

按不同氨基酸模式配制肉鸭日粮的比较研究

试验比较了不同氨基酸模式对肉鸭生产性能的影响。将320只1日龄的樱桃谷鸭随机分为4组,每组80只,设4个重复,每个重复20只,公母各半,分别饲喂四种日粮(按四种氨基酸模式配制),试验期21天。结果表明:日粮的氨基酸模式为Lys∶Met∶Trp∶Thr=100∶45∶21∶62时,其2周和3周体重高于其它各组,但差异不显著(P>0.05),且F/G和血清尿酸水平最低。     

不同培养基对杏鲍菇菌丝生长的影响

比较了杏鲍菇一级种和二级种培养基配方 ,试验表明杏鲍菇一级种培养基以配方三最好 ,菌丝干重是配方七 (对照 )的 3 5倍 ,差异极显著 ;杏鲍菇二级种培养基以配方四最好 ,菌丝生长势最强 ,与供试各培养基配方相比较 ,差异均为极显著     

Antitumor effect of chlorophyllin in vitro

AIM: To study the effect and mechanism of chlorophyllin (CHL) inhibiting HT29 cells. METHODS: IC₅₀ value and growth curve of HT29 cells were detected with MTT method. Apoptosis was detected with Wright-Giemsa staining, FCM and DNA electrophoresis. Telomerase was detected by PCR-ELISA, and protein and mRNA expression of COX-2 gene were detected through RT-PCR and Western blot. RESULTS: CHL inhibited the growth of HT29 in a dose-dependent manner. CHL blocked HT29 cells in G₁ phase but did not induce apoptosis. Different concentration of CHL inhibits the expression of telomerase and COX-2 in HT29 cells. CONCLUSION: CHL inhibited the growth of HT29 cells by inhibiting the expression of telomerase and COX-2 and blocking cells in G₁ phase.     

专家系统在植保上的应用

专家系统在植保上主要应用于病虫害诊断、预测预报、决策管理等领域。植保专家的特点是应用范围广、实用性增强、新技术和新方法不断应用、有向综合性系统发展的趋势。文中还对植保专家系统存在的问题和发展对策进行了讨论。     

半自然条件下几种赤眼蜂及品系对亚洲玉米螟卵寄生能力比较

在半自然条件(田间网罩)下,将人工饲养的玉米螟卵按4、8、16块/株3个处理分别固定在网罩内的玉米植株中上部叶片上,然后每网罩分别引入供试赤眼蜂20头,24 h后更换玉米螟卵块,连续3 d。7 d后调查供寄生的玉米螟卵被寄生率及羽化率。结果表明:在半自然条件下广赤眼蜂伊朗1-1品系虽寄生能力高于其他供试品系,但到第2、3天明显降低;玉米螟赤眼蜂北京6-2-2品系每天均维持一个较平稳的寄生能力,产卵量在时间上的分配比较分散,并在第2、3天显示出比其他品系较高的寄生潜能。广赤眼蜂伊朗1-1品系的卵块寄生数量可随卵块密度的增加而增加,而其他品系则在各处理密度下,寄生数量基本没有变化;从卵粒寄生率看,广赤眼蜂伊朗1-1和吉林1-2两个品系的寄生数量随卵块密度的增加而大幅度提高,其他品系增加幅度较小。     

Induction of tumor-specific cytotoxic T lymphocytes in vitro by dendritic cells pulsed with different glioma antigens

AIM: The goal of this study was to compare different methods for tumor antigen preparation, to observe the induction of tumor-specific cytotoxic T lymphocytes in rats by dendritic cells (DCs) pulsed with different tumor antigens. METHODS: The precursors of dendritic cells were isolated from bone marrow of rats, stimulated in vitro with recombinent rat granulocyte-macrophage colony-stimulating factor (rrGM-CSF) and interleukin-4 (rrIL-4). Then rat DCs were pulsed with C6 tumor cell antigens prepared with different methods: freeze-thaw, boiling or total protein extracted from ultrasonic crushed tumor cell. Subsequently primed DCs were cocultured with T lymphocytes isolated from spleen to induce CTL. Lymphocyte chemoattractant factor from DCs and cytokine IFN-γ release were determined by ELISA, the cytotoxicity of CTL was assayed by JAM test. RESULTS: DCs pulsed with boiled tumor cell in vitro induced an enhanced ability of T-cell proliferation and cytotoxic T lymphocyte activity.CONCLUSION: Our results demonstrated that DCs primed with boiled tumor cell may represent a method for inducing immune responses against the entire repertoire of tumor antigens of malignancies.     

中国美利奴多胎细毛羊及杂交后代多胎性状基因型分析

首次在国内采用了多胎性状基因型诊断法对多胎性状进行选择。试验采集中国美利奴多胎细毛羊及其与Romilly·Hills杂交后代、横交一代样品数分别为 47份、3 8份、2 6份 ,提取DNA确定多胎主效基因FecB 位点 ,酶切检测纯合基因BB、杂合基因B 和非多胎基因 。通过检测 ,三种基因型占样品数百分比分别为 :多胎细毛羊为 2 5 5 3 %、48 94%、2 5 5 3 % ,基因型与产羔符合率为 85 3 7% ;杂交一代为 0、76 3 2 %、2 3 68% ,基因型与产羔符合率为 76 19% ;横交一代为3 0 77%、5 7 69%、11 5 4%。在多胎性状的选择中 ,要坚决剔除非多胎基因个体 ,以缩短选育时间。     

水牛精子在胞质内注射后的早期形态变化

应用胞质内精子注射 (intracytoplasmic sperm injection,ICSI)技术探讨了水牛卵母细胞胞质与注射精子间的相互作用以及 ICSI精子的形态变化。ICSI后 13h,5 9.4 %的精子头部已膨大 ,且有 18.8%的精子进入解聚状态。雌雄原核发育不同步 ,雄原核在 ICSI后 16 h和 19h的形成率分别为 3.2 %和 4 0 .0 % ;雌原核在 ICSI后 13h已达到 71.9% ,16 h时提高到 90 .3%。经离子霉素与 6 - DMAP联合激活 ,ICSI后 19h产生的胚胎核型以 2 PN PB1 为主 ,其雌原核形成 (激活 )率为 91.3% ,雄原核形成率为 4 0 .0 % ,5 1.3%为孤雌胚。用 5 mm ol/ L 的 DTT预处理精子 1h,可提高精子的解聚率 (30 .9%比 12 .7% ,P<0 .0 5 ) ,但对雄原核的形成率无显著影响 (33.3%比 32 .7% ,P>0 .0 5 )。结果表明 ,水牛精子 ICSI后的雄原核形成时间晚于卵子雌原核形成时间 ,且其比率低于卵子 ,DTT处理精子能提高其解聚率 ,但对雄原核形成无影响