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MARC R. DESJARDINS DMV DVSC MARK B. HURTIG DVM MVSC Dipiomate ACVS NIGEL C. PALMER DVM PhD 《Veterinary surgery : VS》1991,20(6):434-445
Two 10 mm thick osteochondral grafts were harvested from the lateral aspect of the lateral trochlear ridge of the left talus in each of 10 anesthetized horses. The grafts were frozen in a 7.5% DMSO solution and stored in liquid nitrogen. The horses were anesthetized again on day 14 and the thawed grafts were press-fitted into drill holes in the trochlear ridges of the right stifle. A fresh graft was transferred from the right hock to the left stifle. To control for the effects of surgery, another fresh graft was transferred from the right stifle to the left stifle. The result was two grafts in each femoropatellar joint. Fresh and frozen osteoarticular autografts appeared to maintain a durable weight-bearing surface for 3 months; however, the fresh grafts were clearly superior. Frozen grafts had fewer living chondrocytes, decreased safranin-O staining, and decreased SO435 uptake. Graft stability and articular surface congruency were determining factors in the outcome of all grafts. Since the availability of osteochondral autografts is limited, further work on the use of preserved allogeneic osteochondral tissue is warranted. 相似文献
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C. E. LAYTON DVM MS H. R. FERGUSON DVM PHD DIPLOMATEACVS J. E. COOK DVM PHD DIPLOMATEACVP M. M. GUFFY DVM MS DIPLOMATEACVR 《Veterinary surgery : VS》1987,16(2):175-182
Three intrapelvic urethral anastomosis techniques were performed on 12 mature male dogs to compare the degree of stricture. The intrapelvic urethra was transected 1 cm caudal to the prostate, and anastomosis was performed using either suturing of the urethra over an indwelling catheter, suturing of the urethra without an indwelling catheter, or apposition of the urethra without sutures over an indwelling catheter. Postoperatively, the dogs were evaluated using clinical urination patterns, biochemical tests, radiography, and pathology. Three of four suture-catheter dogs and one of four catheter-only dogs had normal urinary patterns. Stricture (25-84%) with urethral dilation or fistulas tracts was visible on retrograde, positive contrast urethrograms of 3 of 12 intact dogs 20 weeks after surgery, and on all 12 excised lower urinary tracts. Suture-catheter dogs subjectively had the least amount of stricture. All eight dogs (1 suture-catheter dog, 4 suture-only dogs, and 3 catheter-only dogs) with severe stricture (greater than 60% lumen diameter reduction) had histopathologic signs of chronic, inflammatory urinary tract disease, including four with chronic, ascending lymphocytic pyelonephritis. Hydroureter and hydronephrosis developed in two catheter-only dogs. Complete urethral transection resulted in some degree of stricture, regardless of technique used for anastomosis. Urethral anastomosis over an indwelling catheter appeared to result in a lesser degree of stricture and clinical and histopathologic derangement. 相似文献
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Abstract Rainbow trout were fed practical-type diets containing 0.6, 6.6 and 114 μ g/g selenium for 16 weeks at approximately 15°C. Fish reared on the highest levels of dietary selenium had a significant reduction in final body weight, feed: gain ratio and increased mortality. Haematocrit, plasma calcium, glucose, and protein levels did not vary significantly. Ninety per cent of the fish fed the diet containing the highest level of selenium developed nephrocalcinosis. Fish with occult nephrocalcinosis had significantly increased levels of kidney calcium and those with overt nephrocalcinosis had significantly increased levels of kidney calcium and magnesium, and liver magnesium. Microscopically, the majority of renal damage was tubular with the renal interstitium becoming inflamed only when tubules degenerated. The pathogenesis of selenium-induced nephrocalcinosis is discussed. 相似文献
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The Power of a Statistical Test What Does Insignificance Mean? 总被引:1,自引:0,他引:1
In statistical testing of data, the p value is a standard measure for reporting quantitative results. When a significant difference is reported, (e.g., P less than .05), most readers understand that there is less than a 5% chance that the authors have made a type I error (false positive or alpha) with their conclusion. In contrast, when nonsignificant differences between treatments, groups, or parameters of interest are reported (e.g., P greater than .05), many investigators and readers incorrectly interpret the 95% confidence interval for this conclusion as a 95% chance of making the correct decision. In fact, the alpha level of significance (in this example, .05) is only one of the parameters that determines the probability of committing a type II error (false negative or beta) when concluding statistical insignificance. Statistical power is the probability of having made a correct decision when the statistical tests reveal insignificance (P greater than .05) and the null hypothesis is true. The higher the power, the greater the chance that the decision is correct. Power depends on the alpha level of significance, the sample size, the standard deviation of the population or the sample, and the magnitude of the difference the investigators are trying to demonstrate. 相似文献
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