由体外受精囊胚获得ICR小鼠ES细胞

收集鼠卵母细胞和精子，采用体外受精技术，获得早期胚胎。体外培养胚胎至囊胚，分离内细胞团，获得ICR小鼠的ES细胞。结果不但获得了来自体外受精囊胚的ICR小鼠ES细胞，而且表明用体外受精方法获得的小鼠早期胚胎的数量明显大于常规方法，且比较稳定。ICR小鼠ES细胞的获得，为研究小鼠的ES细胞分化提供了条件。     

添加中草药对饲料蛋白质消化率的影响

试验采用体外消化法研究添加不同剂量的两种中草药对饲料蛋白质消化率的影响.选用陈皮、山楂两种中草药,然后在体外环境下模拟猪胃肠道消化环境进行测定.结果表明:(1)添加中草药陈皮A组(0.5%)和C组(1%)的消化残渣蛋白质质量和粗蛋白质消化率与对照组相比差异极显著(P＜0.01),而B组(0.75%)与对照组相比差异显著(P＜0.05).(2)添加中草药山楂D组(0.5%)、E组(0.75%)、F组(1%)三个组的消化残渣蛋白质质量和粗蛋白质消化率与对照组相比差异极显著(P＜0.01).(3)山楂的效果明显好于陈皮,且山楂添加量为0.75%时最好.     

铁苋菜提取物对仔猪生长性能、血液生化指标及肠道菌群的影响

目的:本试验旨在研究铁苋菜提取物对仔猪生长性能、血液生化指标及粪样菌群的影响.方法:选取同一批次的25日龄健康的外三元断乳仔猪120头,平均体重为9.60+0.65 kg/头,随机分为4组,每组3个重复,每个重复10头,公母各半;试验组分别为铁苋菜提取物组Ⅰ和组Ⅱ、抗生素组及对照组,铁苋菜提取物组Ⅰ和组Ⅱ在基础日粮中分...     

几种重要饲草的脂肪酸成分分析

对紫花苜蓿、苇状羊茅、鸭茅、无芒雀麦等12个饲草样品进行了脂肪酸含量的测定,并与豆油、菜籽油和棉籽油等植物油脂肪酸成分进行了比较。结果表明,饲草中的脂肪酸以亚麻酸为主,亚油酸次之,其它脂肪酸很少。植物油中的脂肪酸以油酸和亚油酸为主,亚麻酸很少。以饲草为主配合少量植物油饲喂奶牛有利于乳脂中共轭亚油酸的合成。     

高羊茅幼穗分化过程及开花习性的观察

为阐明高羊茅(Festuca arundinacea Schreb.)的生殖生长过程,2008年4月下旬对高羊茅幼穗分化过程和开花习性进行观察。结果表明:高羊茅幼穗分化是一个连续的过程,可分为苞原基分化期,1次枝梗原基分化期,2、3次枝梗原基分化期、小穗及小花原基分化期、雌雄蕊形成期5个时期;高羊茅开花的规律性很强,总穗轴最上部的小穗最先开花,然后向下进行,基部小穗最后开花;每个枝梗最上部的小穗最先开花,其次是该枝梗基部小穗开花,然后向上进行,上部数第2个小穗最后开花;小穗则是基部小花最先开放,然后向上顺序开放。     

非肌肉肌球蛋白Ⅱ型重链A单克隆抗体的制备及其靶位点区域的鉴定

为制备非肌肉肌球蛋白Ⅱ型重链A(NMHC-II A)的单克隆抗体(MAb),本研究将编码NMHC-Ⅱ A羧基端1 651～1 960氨基酸的表达重组质粒转化到大肠杆菌感受态细胞Rosseta中诱导表达,重组蛋白纯化后免疫BALB/c小鼠,分离小鼠脾细胞与SP2/0骨髓瘤细胞用PEG 4000介导融合。ELISA方法检测筛选阳性杂交瘤细胞,制备腹水并纯化MAb,以间接免疫荧光和免疫沉淀方法鉴定MAb的特异性,western blot检测MAb靶位点区域。结果表明,通过筛选得到1株稳定分泌抗体的杂交瘤细胞系,其抗体亚型为IgG1,细胞培养上清和腹水效价分别为1∶2 560和1∶1.0×106;该MAb能够特异性结合相应抗原,并结合Marc-145细胞;western blot结果显示该抗体与NMHC-Ⅱ A的1 812～1 894氨基酸位点结合。该抗体的制备为研究NMHC-Ⅱ A在猪繁殖与呼吸综合征病毒感染细胞中的作用奠定了基础。     

柴达木盆地次生盐渍化撂荒地的改良与利用

在分析了柴达木盆地次生盐渍化撂荒地的成因的基础上，对生物改良和利用撂荒地的大量试验结果进行了分析和总结，提出了封育一灌溉和建植入工草是改良与利用盐渍化撂荒化撂荒地的有效途径，并阐述了在柴达木盆地建立草地农业生态系统的必要性 。     

Effects of chromium nicotinate on performance, carcase characteristics and blood chemistry of growing turkeys

1. The objective of this trial was to study the effects of chromium nicotinate (Cr) supplementation on the performance, carcase characteristics and blood constituents of 9 to 22 week-old male turkeys. 2. Fifty-four 8-week-old male turkeys weighing 2.9 +/- 0.28 kg were allocated at random into 9 groups with 3 dietary treatments and 3 replicates. The dietary treatment was different dietary concentrations of chromium in the basal diet, to provide 0 (control), 1 or 3 mg/kg. The feeding trial lasted for 14 weeks. 3. Results showed that 1 mg/kg chromium supplementation significantly improved weight gain and food intake at 9 to 18 weeks of age but did not significantly influence performance at 19 to 22 week of age. The breast and thigh muscle were significantly increased in birds receiving 1 mg/kg chromium supplementation but were decreased by 3 mg/kg of supplementation. 4. Dietary chromium supplementation did not significantly influence other serum constituents, including insulin, HDL, VLDL+LDL, HDL-C, VLDL-C+LDL-C, total protein, albumin, and gamma-globulin at 18 and 22 weeks of age. 5. The serum triacylglycerol (TG) and uric acid contents were significantly increased, while glycerol and alpha-globulin were significantly reduced by 3 mg/kg chromium supplementation. However, 1 mg/kg chromium supplementation significantly reduced serum cholesterol and glycerol at 18-weeks old. At 22 weeks of age, 1 mg/kg chromium supplementation significantly increased serum glucose and decreased the uric acid concentration whereas 3 mg/kg chromium supplementation significantly increased the creatinine concentration and decreased beta-globulin concentration.     

东北虎雌性生殖系统解剖

对 2例 1 999年 1 0月意外死亡的东北虎雌性生殖系统进行了解剖。雌性东北虎的卵巢被覆卵巢囊 ,随着年龄增长卵巢表面凸凹不平 ,呈脑的沟回状。输卵管位于卵巢游离缘外侧 ,呈半圆形围绕着卵巢 ,输卵管壶腹部弯曲、峡部直 ;子宫与输卵管分界明显 ;子宫角长度约相当于子宫体长度的 2倍 ;阴道呈背腹压扁的管状