Identification,Characteristics and Function of Phosphoglucomutase (PGM) in the Agar Biosynthesis and Carbon Flux in the Agarophyte Gracilariopsis lemaneiformis (Rhodophyta)

Agar is widely applied across the food, pharmaceutical and biotechnology industries, owing to its various bioactive functions. To better understand the agar biosynthesis in commercial seaweed Gracilariopsis lemaneiformis, the activities of four enzymes participating in the agar biosynthesis were detected, and phosphoglucomutase (PGM) was confirmed as highly correlated with agar accumulation. Three genes of PGM (GlPGM1, GlPGM2 and GlPGM3) were identified from the G. lemaneiformis genome. The subcellular localization analysis validated that GlPGM1 was located in the chloroplast and GlPGM3 was not significantly distributed in the organelles. Both the GlPGM1 and GlPGM3 protein levels showed a remarkable consistency with the agar variations, and GlPGM3 may participate in the carbon flux between (iso)floridoside, floridean starch and agar synthesis. After treatment with the PGM inhibitor, the agar and floridean starch contents and the activities of floridean starch synthase were significantly decreased; products identified in the Calvin cycle, the pentose phosphate pathway, the Embden-Meyerhof-Parnas pathway and the tricarboxylic acid cycle were depressed; however, lipids, phenolic acids and the intermediate metabolites, fructose-1,6-phosphate were upregulated. These findings reveal the essential role of PGM in regulating the carbon flux between agar and other carbohydrates in G. lemaneiformis, providing a guide for the artificial regulation of agar accumulation.     

联苯菊酯10%水乳剂的配方研究

本研究通过正交优化得到联苯菊酯10%水乳剂的最佳配方：联苯菊酯原药10%,二甲苯20%,农乳602#3.5%,Tween-20 2.5%,农乳500#2%,乙二醇4%,有机硅消泡剂0.2%,去离子水补足至100%。最佳生产工艺为：使用高速剪切机,剪切转速6 000r/min,剪切时间4min。该水乳剂pH值5.7～6.5,稀释100倍于（30±2）℃恒温水浴24h未出现浮油、沉油、沉淀,经（54±2）℃热贮14d,分解率〈2%,制剂表面张力低,倾倒性佳。     

咪鲜胺·抑霉唑混配制剂的反相离子对液相色谱分析

本文采用反相离子对色谱梯度洗脱外标法对咪鲜胺．抑霉唑乳油的2种有效成分进行一次性定量检测,采用C18色谱柱,以0．012mol／L庚烷磺酸钠(pH=2．5) 甲醇=30 70;20 80为流动相,流速1．5mL／min,二极管阵列检测器,检测波长：220nm。该方法中咪鲜胺和抑霉唑的标准偏差分别为0．065和0．024,变异系数分别为0．53％和1．13％．线性相关系数分别为0．9994和0．9995,平均回收率分别为99．4％、100．1％。     

通过考核认证提高警犬实战技能

2006年,辽宁省公安厅作为片区警犬资格认证的试点单位,分别于5月17日至6月12日、11月8日至9日,两次集中组织进行了警犬资格认证考核。全省公安机关共有133头警犬参加考核,占全省163     

经验学习对警犬技术成人培训的影响

所谓经验学习就是学习者通过对自己过去所掌握的知识和经验的深刻回顾与系统反思,结合需要学习的新知识、新内容,从中总结归纳并产生出新知识的过程。实践证明,经验学习对警犬技术专业成人培训的影响是巨大而深刻的,有时往往会超出人     

Comparing surface erosion processes in four soils from the Loess Plateau under extreme rainfall events

This research aims to improve erosion control practice in the Loess Plateau, by studying the surface erosion processes, including splash, sheet/interrill and rill erosion in four contrasting soils under high rainfall intensity (120 mm h⁻¹) with three-scale indoor artificial experiments. Four contrasting soils as sandy loam, sandy clay loam, clay loam and loamy clay were collected from different parts of the Loess Plateau. The results showed that sediment load was significantly impacted by soil properties in all three sub-processes. Splash rate (4.0–21.6 g m⁻²∙min⁻¹) was highest in sandy loam from the north part of the Loess Plateau and showed a negative power relation with the mean weight diameter of aggregates after 20 min of rainfall duration. The average sediment load by sheet/interrill erosion (6.94–42.86 g m⁻²∙min⁻¹) was highest in clay loam from middle part of the Loess Plateau, and the stable sediment load after 20 min showed a positive power relation with the silt content in soil. The average sediment load increased dramatically by rill and interrill erosion (21.03–432.16 g m⁻²∙min⁻¹), which was highest in loamy clay from south part of the Loess Plateau. The average sediment load after the occurrence of rill showed a positive power relation with clay content and a negative power relation with soil organic matter content. The impacts of slope gradient on the runoff rate and sediment load also changed with soil properties. The critical factors varied for different processes, which were the aggregate size for splash erosion, the content of silt particles and slope gradient for sheet/interrill erosion, and the content of clay particles, soil organic matter and slope gradient for rill erosion. Based on the results of the experiments, specific erosion control practices were proposed by targeting certain erosion processes in areas with different soil texture and different distribution of slope gradient. The findings from this study should support the improvement of erosion prediction and cropland management in different regions of the Loess Plateau.     

羊绒与羊毛的PCR-RFLP识别方法

羊绒价格昂贵,在销售中时常出现掺假现象,最常见的是用羊毛,因此精确识别羊绒和羊毛纤维的方法非常重要。本试验采用聚合酶链式反应-限制性片段长度多态（PCR-RFLP）的技术方法,利用提取的羊绒和羊毛线粒体基因组,根据细胞色素b（Cytochrome b）基因已知的DNA序列设计引物,通过内切酶SspⅠ进行酶切,得到不同的片段长度,根据酶切图谱鉴别羊绒和羊毛样品,用来进行羊绒掺假试验的定性检测。     

含PRRS病毒ORF5的伪狂犬病病毒TK基因缺失转移载体的构建

提取伪狂犬病病毒（ＰＲＶ）ＢａｒｔｈａＫ６１株基因组ＤＮＡ,用限制性内切酶ＫｐｎＩ充分消化,回收５．９ｋｂ片段（Ｊ片段）,将其克隆于质粒ｐＵＣ１１９ ＫｐｎＩ位点上,获得ｐＢＫＪ。用两对针对ＰＲＶ ＴＫ基因的特异性引物对重组质粒进行ＰＣＲ鉴定,证明其中含有ＰＲＶ ＴＫ基因。然后用ＫｐｎＩ、ＰｓｔＩ和ＢａｍＨＩ等限制性内切酶对其进行酶切分析,确定了克隆片段的物理图谱。进一步研究证实ＴＫ基因位于其中的     

间接法Dot－ELISA检测传染性法氏囊病病毒的研究

本文应用间接法Ｄｏｔ－ＥＬＩＳＡ进行鸡传染性法氏囊病病毒（ＩＢＤＶ）抗原的检测，其最低检出量为０．４３μｇ／ｍｌ。对人工感染／自然病例的各组织脏器进行检测，结果法氏囊的ＩＢＤＶ检出率为１００％，脾脏的检出率为８０％／８４．９％，其它器官组织如肾、胸腺、心、肝、肺、盲肠扁桃体、胸肌等均含有一定量的病毒（１１～６０％）。经统计学分析，法氏囊与脾脏的病毒检出率之间具有显著性差异（人工感染差异极显著Ｐ＜０．０１，自然病例差异显著Ｐ＜０．０５），进一步证实法氏囊是ＩＢＤＶ最主要的靶器官。试验结果表明，该法检测ＩＢＤＶ抗原，敏感性高、特异性强、重复性好，并且经济、方便、快速，适于大批量样品检测，可作为一种诊断ＩＢＤ的比较理想的方法。     

不同收获期玉米青贮营养成分在奶牛瘤胃内降解率的研究

本文旨在研究不同收获期玉米青贮对奶牛的营养价值。用3头装有永久性瘤胃瘘管的中国荷斯坦奶牛,采用3×3拉丁方设计对蜡熟期、乳熟期和乳熟前期的玉米青贮的主要营养成分进行了瘤胃降解率的研究。结果表明:(1)蜡熟期玉米青贮DM有效降解率(60.07%)分别比乳熟期(52.91%)和乳熟前期(45.13%)高7.16%(P<0.05)和14.94%(P<0.05);乳熟期比乳熟前期高7.78%(P<0.05)。(2)乳熟期玉米青贮NDF的有效降解率(30.77%)分别高于蜡熟期(28.59%)和乳熟前期(26.71%,P<0.05),蜡熟期和乳熟前期之间差异不显著。(3)蜡熟期ADF(25.73%)和乳熟前期玉米青贮(25.23%)没有明显差异,但都高于乳熟期(23.39%,P<0.05)。(4)蜡熟期玉米青贮CP有效降解率(81.93%)分别比乳熟期(76.43%)和乳熟前期(73.36%)高5.50%(P<0.05)和8.57%(P<0.05);乳熟期比乳熟前期高3.07%(P<0.05)。通过试验综合评价不同收获期玉米青贮对奶牛的营养价值是蜡熟期玉米青贮优于乳熟期,乳熟期玉米青贮优于乳熟前期。