Evaluation of carbon sources,gelling agents,growth hormones and additives for efficient callus induction and plant regeneration in Indian wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis> L.) genotypes using mature embryos

Various factors affecting in vitro regeneration like different carbon sources, different gelling agents, and growth additives were assessed comprehensively for callus induction and plant regeneration for five Indian wheat cultivars using mature embryos as the explants for the first time. The tissue culture responses of cultivars WH-1105, HD-2967, and PBW-343 have not been reported earlier. Besides, the effect of different concentrations of the cytokinin, zeatin has also been optimized. Using the optimized factors, the efficiency of five different varieties, i.e., HD 2967, C 306, RAJ 3765, WH 1105, and PBW 343 was evaluated for regeneration. Modified MS basal medium containing dicamba reduced precocious germination of the embryo and induced embryogenic callus more efficiently. Removal of embryogenic calli from non-regenerable structures during early callus phase improved plant regeneration. These calli on zeatin (1.0 mgl^-1) and dicamba (0.1 mgl^-1) containing medium showed the highest regeneration frequency (98%) with a maximum of 8-9 shoots per calli. Maltose had the maximum callusing and regeneration percentage than other carbon sources. Various gelling agents did not have any significant difference on the regeneration. Of all the varieties, C-306 and HD-2967 were found to be more regenerative and can be used in transformation experiments.     

播期对苏北盐碱地不同水稻品种生长发育进程及产量的影响

为了探究播期对苏北盐碱地环境下不同水稻品种的生长发育进程及产量的影响,本试验采用大田小区试验,调查和比较不同播期下‘南粳9108’和‘连粳11号’的生育期、干物质积累量、株高和产量等相关性状。结果表明：随播种时间推迟,‘南粳9108’和‘连粳11号’的全生育期变短;‘南粳9108’的拔节期、齐穗期和成熟期的干物质重呈减小趋势,‘连粳11号’呈先升后降趋势;播期对‘连粳11号’株高影响差异显著,且株高随播期推迟呈下降趋势;‘南粳9108’和‘连粳11号’的播种时间推迟,其有效穗、每穗粒数和产量呈下降趋势。‘南粳9108’在苏北盐碱地区的最佳播种时间为5月24日,‘连粳11号’为5月31日。     

电化学酶传感器在有机磷农药残留检测中的应用

从识别有机磷农药的酶及识别机理、酶的固定化技术、酶固定化载体等不同角度,综述了有机磷农药电化学酶传感器的研究概况,并展望了其发展前景。     

基于图像分析方法的水稻根系形态特征指标的定量分析

利用图像分析方法定量研究水稻根系形态特征指标的变化规律。通过实施不同水氮处理和不同品种的水稻桶栽试验,选用WinRhizo (WR)软件的不同模式(LM和T200)分析根系图像,分别提取根直径范围0~0.30 mm和0.25~1.80 mm的根系形态数据,并与基于Image-Pro Plus 6.0软件(IP)的根直径和不定根长的测量结果进行比较分析;确定了3种水稻品种不同类型根直径的范围;对不同生育时期水稻根长、根体积、根表面积、根系干重和不定根数量等水稻根系形态特征指标变化差异,以及分枝特征进行了分析。结果表明：(1)基于WR软件LM和T200模式下的根直径测量结果与基于IP软件的测量结果之间NRMSE分别为5.44%和10.95%。(2)界定细分枝根直径范围为0.03~0.10 mm,扬稻6号(V3)粗分枝根和不定根分别为0.10~0.30 mm和0.30~1.65 mm,而日本晴(V1)和武香粳14(V2)分别为0.10~0.25 mm和0.25~1.40 mm,不定根长与IP测量结果进行比较,其NRMSE为10.78%~12.01%。(3)各指标抽穗前增长迅速,之后增长减缓或衰老下降;不同氮肥处理间各指标分蘖至成熟均差异显著,增施氮肥可促进根系生长,明显提高不定根比例;适当控水可促进根系生长,明显提高分枝根比例;品种V3自分蘖期到成熟期各指标均显著高于V1和V2,V1与V2间差异不显著。本研究表明,本方法具有较好的精度和可行性,为推进水稻或其他作物根系的定量研究提供了参考。     

五香兔肉加工技术的研究

通过使用不同作料,不同加工方法,研究了兔肉的加工工艺。研究出独特的辅料、作料及加工工艺,生产出色、香、味俱佳的五香兔肉,其产品在市场上很受欢迎。     

洋水仙花粉生活力的测定方法初探

以洋水仙品种‘Albany’、‘Mount Hood’、‘Geranium’的新鲜花粉为试材,采用TTC染色法、醋酸洋红染色法和离体萌发测定法来测定其生活力,以探寻测定水仙花粉生活力的最适方法。结果表明：醋酸洋红染色法和离体萌发测定法均能够准确测定出水仙花粉生活力,而TTC染色法不适合用于洋水仙花粉活力的测定,其中醋酸洋红染色法适用于洋水仙的花粉生活力快速测定,而离体萌发测定法则适合于洋水仙的花粉生活力准确测定。经过对9种培养液组合的方差分析显示：0.1g/ml蔗糖+0.1×10-3g/ml硼酸为最佳培养液组合。     

基于PSR模型框架下旅游发展对民族传统文化影响机制分析

民族旅游开发对民族传统文化的影响,既有积极促进的一面,也有消极阻碍的一面。分析旅游业发展对民族传统文化产生影响的原因、过程、演化,尽可能降低其消极影响,扩大积极影响,使民族地区旅游发展与民族传统文化达到“双赢”,是民族地区旅游业可持续发展的要求。     

苹毛丽金龟性信息素嗅觉行为与释放模式

苹毛丽金龟在我国很多地区是重要经济害虫。在实验室利用采集的两性及寄主挥发物测试雌雄行为反应,结果表明,苹毛丽金龟通讯行为受寄主植物和性信息素调控。添加了雌性挥发物的寄主植物挥发物,能增加对雄虫的吸引力。喂食与不喂食李子花对性信息素释放具有显著影响。气相色谱及行为学研究证实至少有一种物质作为性信息素存在于雌性挥发物中。光期开始后4~6小时,是雌性信息素释放的高峰期。     

Three AFLP markers tightly linked to the genic male sterility <Emphasis Type="Italic">ms</Emphasis><Subscript><Emphasis Type="Italic">3</Emphasis></Subscript> gene in chili pepper (<Emphasis Type="Italic">Capsicum annuum</Emphasis> L.) and conversion to a CAPS marker

Genic male sterility (GMS) has long been used as a tool for hybrid seed production in chili pepper (Capsicum annuum L.). We developed DNA markers linked to the GMS ms ₃ gene in a segregating population using bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) techniques. The segregating population was subjected to BSA-AFLP with 512 primer combinations. Three AFLP markers (Eagg/Mccc₂₇₆, Eagc/Mctt₁₇₈, and Ecag/Mtgc₂₀₄) were identified as tightly linked to the ms ₃ locus. Among them, we converted the AFLP marker Ecag/Mtgc₂₀₄ to the cleavage amplified polymorphic sequence (CAPS) marker, named GMS3-CAPS, based on sequencing analysis of internal and flanking regions for the markers between male-fertile and sterile plants. This marker will be useful for pepper breeding using the GMS system.     

An optimal equivalent subchannel algorithm for low voltage power line communication system

According to the low voltage power line channel features,combined with adaptive bit loading and power allocation algorithm,an optimal equivalent subchannel algorithm is proposed.Under the condition of fixed amount of transmitted bits and the minimum transmitted power model,impact of optimal equivalent subchannel on the system performance is studied.The optimization algorithm sorts subchannels with similar transmission features into groups,thus the signaling load can be reduced,and the spectrum can be better used.The simulation results show that system can get lower bit error rate(BER) by adopting optimal equivalent subchannel algorithm.