豆芽烂芽的病原菌分离鉴定及致病性研究

对规模化生产中的绿豆芽烂芽取样,进行致病菌的分离、纯化及鉴定,确定病原菌为瓜果腐霉,观测了不同接种量的瓜果腐霉孢子悬浮液对绿豆芽的致病性。结果表明,不同接种量处理,其发病时间、发病率、豆芽生物产量均存在差异。接种浓度为10^3个／mL和104个／mL的抱子悬浮液处理后,豆芽烂芽严重,接种浓度10^3个／mL处理第3天出现烂芽,发病率为10％,第4,5天发病率分别为58％,92％;接种浓度10^4个／mL处理第2天开始出现烂芽,发病率为2％．第3,4,5天发病率分别为58％,100％,100％,发病情况日趋严重;发病芽体呈水浸状、浅褐色腐烂,有刺激性异味：严重影响其生物产量,生物产量与健康幼苗相比分别降低33．1％,33．3％。     

无籽西瓜新品种新优38号的选育

新优38号为中早熟无籽西瓜一代杂种,母本S401-1是利用秋水仙碱诱变获得的四倍体自交系,父本H201是从二倍体材料中选出的抗病、优质自交系。该品种全生育期90 d(天)左右,果实发育期32 d(天)左右;果实椭圆形,新疆地区露地栽培果形指数1.25左右,南方地区露地栽培果形指数1.15左右;果皮浅绿色覆深绿色宽条带,光滑美观,果皮厚约1.0 cm;瓤鲜红色,质脆多汁,口感风味好,中心可溶性固形物含量11.4 %左右,白色秕籽小而少;单果质量4～6 kg,每667 m2产量3 500 kg左右。     

芒果采后及贮藏生理研究进展

芒果是跃变型水果。采收后果实品质和代谢生理等发生重大变化,笔者从芒果果实的采后品质变化、呼吸强度、乙烯代谢、活性氧代谢、Ca2+对果实后熟的影响、逆境生理及不同采后处理对芒果采后生理的影响等方面,阐述了芒果采后和贮藏过程中的主要生理变化的研究进展,并指出了今后芒果采后保鲜的研究方向和研究内容。     

Effects of five cryoprotectants on proliferation and differentiation‐related gene expression of frozen‐thawed bovine calf testicular tissue

The cryopreservation of testicular tissue is a potential method for preserving male fertility. However, the effect of cryopreservation on bovine calf testicular tissue is scarce. This study investigated the effect of different cryoprotectants on bovine calf testicular tissue at the molecular level. Testicular tissue from ten immature bovine calves (6 months) was collected after slaughter and cryopreserved in an extender containing different concentrations of the following five cryopreservation solutions (CP): bovine serum albumin (BSA) with 5% dimethyl sulfoxide (DMSO), trehalose with 5% DMSO, DMSO and glycerol and ethylene glycol (EG). After 7‐day cryopreservation, the expression levels of three spermatogonial stem cell (SSC)‐related genes, octamer‐4 (OCT4), KIT ligand (MGF/SCF) and kit oncogene (C‐KIT), were investigated by quantitative PCR (qPCR). The cell viability was highest for the tissues preserved with 30 mg/ml BSA (77.82% ± 1.22) and 40 mg/ml trehalose (74.23% ± 1.16) compared with other groups (p < 0.05), and the level of expression of the three genes was highest with 30 mg/ml BSA (p < 0.05). Compared with other CPs, the 30 mg/ml BSA and 40 mg/ml trehalose have the better cryopreserve protection. The 30 mg/ml BSA is the most viable media for the cryopreservation of testicular tissue from cattle.     

硝化应激在神经型犬瘟热疾病发展中的临床意义

为了研究硝化应激在神经型犬瘟热疾病发展中的作用,并探讨其对该病的诊断价值,选取犬瘟热临床病例113例,其中有神经症状组(NCD)共37例,无神经症状组(NNS)共76例,并设25例无犬瘟热健康犬作为对照组(CG).就诊时分别采取血液和脑脊液,测定一氧化氮(NO)、神经元型一氧化氮合成酶(nNOS)和神经元特异性烯醇化酶...     

对动物卫生防疫条件审核实行量化分级管理的探讨

新修订的《中华人民共和国动物防疫法》于2008年1月1日起生效执行。该法第二十条规定:兴办动物饲养场(养殖小区)和隔离场所,动物屠宰加工场所,以及动物和动物产品无害化处理场所,应当向县级以上地方人民政府兽医主管部门提出申请,     

蔬菜中重金属特征研究

重金属在蔬菜中的含量随不同的蔬菜种类和品种差异明显。重金属平均含量明显较高的蔬菜品种有苋菜、莴笋、生菜、葱子;变异系数较大的蔬菜品种有莲白、海椒、葱子。叶菜类中大部分重金属含量高于果菜类。不同蔬菜种类相关性分析表明相关性程度为叶菜类＞果菜类,不同蔬菜品种相关程度为莴笋＞莲白＞四季豆＞葱子＞海椒＞生菜＞苋菜。重金属群集分析表明不同蔬菜品种重金属间的协同、拮抗作用差异较大,同类蔬菜品种重金属含量具有相似性。同国家食品卫生限量标准比较发现超标的蔬菜品种主要为叶菜类,超标地区主要在近郊区,超标的重金属主要为Pb、Cd。     

茄子新品种新茄5号的选育

新茄5号为中晚熟茄子一代杂种,其母本19号茄是由五叶茄经多年自交选育出的稳定自交系,父本18号茄是由灯笼红茄经多代自交选育出的稳定自交系。植株生长势强,第1花出现在第6~7节,果实近圆形,果脐小,深紫红色,有光泽,平均单果质量600 g,商品性好。抗黄萎病能力优于灯笼红茄和五叶茄,一般每667 m2产量6 000 kg左右,适于新疆地区早春露地覆膜栽培,也适于早莴苣、甘蓝、花椰菜收获后秋延后栽培。     

罐藏番茄新品种红杂18的选育

红杂１８是以红２１３为母本，黄苗试材９３７５为父本，配制成适宜罐藏加工和长途运销的番茄一代杂种。具有高抗ＴＭＶ、中抗ＣＭＶ、果实硬度高、抗裂、耐压、耐贮运、果实加工性状优良等特点。已在新疆、甘肃、宁夏、广西、云南等省区的加工番茄生产基地和远运外销生产基地推广６４９．８ｈｍ２。     

Pathogen Analysis of Goat's Respiratory Diseases

To investigate the pathogens of goat's respiratory disease from a goat farm in Guangxi province, the epidemiological investigation, clinic observation, pathological examination, bacterium isolation and identification, biochemical test, PCR and animal experiment test were conducted.The preventive and control measures were taken on the basis of pathogen epidemiological characteristics and drug sensitive test results.The results showed that Mycoplasma, influenza virus and parasite were negative by isolation and culture or PCR, however, two strains of gram-negative bacteria named MS1 and MS2 were isolated from lung.MS1 was identified as Serratia marcescens by biochemical test and 16S rRNA sequencing, which shared 99% nucleotide homology with other Serratia marcescens in GenBank.And MS2 was identified as E.coil by the same methods that shared 99% nucleotide homology with other E.coli in GenBank.Animal experiment showed that two strains could cause death in mice.The drug sensitive tests showed two strains were highly sensitive to spectinomycin, amikacin, kanamicin and neomycin.Kanamycin and dexamethasone were used to treat the sick goats, and achieved good results.