Molecular cloning and functional analysis of duck Toll-like receptor 5

Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In this study, we cloned the single-exon TLR5 gene of the Maya breed of Common Shelduck (Tadorna tadorna). The TLR5 open reading frame is 2580 bp in length and encodes an 859-amino acid protein. The putative amino acid sequence of duck TLR5 consisted of a signal peptide sequence, 11 leucine-rich repeat domains, a leucine-rich repeat C-terminal domain, a transmembrane domain, and an intracellular Toll-interleukin-1 receptor domain. The duck TLR5 gene was highly expressed in the lung, bone marrow, spleen, and liver; moderately expressed in kidney, small intestine, large intestine, and brain. A plasmid expressing duck TLR5 was constructed and transfected into HEK293T cells, and expression was confirmed by indirect immunofluorescence assay. HEK293T cells transfected with duck TLR5- and NF-κB-luciferase-containing plasmids significantly responded to flagellin from Salmonella typhimurium, indicating that it is a functional TLR5 homolog.     

激素与营养液配施对平菇产量及品质的影响

植物激素与营养液配合施用可促进平菇菌丝生长和子实体发育，提高产量，改善商品性状，但却不能增加干物质的积累。对营养成分含量的影响随激素与营养液配方的不同而异。     

The role of ectomycorrhizal fungi in alleviating pine decline in semiarid sandy soil of northern China: an experimental approach

The decline of Mongolian pine (Pinus sylvestris var. mongolica) trees on sandy land in northern China has caused serious ecological concerns. Mongolian pine is an ectomycorrhizal fungus (ECM)-dependent species. Three ECM species (Boletus sp., Lactarius deliciosus and L.sp.) were collected from Mongolian pine plantation stands to test their beneficial effects on Mongolian pine seedlings and their responses to environmental factors such as pH, drought stress and temperature. The results indicated that ECM inoculation significantly increased the rate of ECM colonization and the length of seedling shoots. The three ECM could grow in a pH range from 4 to 7, but did not grow under heavy drought stress (?1.53 MPa). High temperatures (over 37 °C) caused death of ECM. When related to soil pH, soil water content and temperature in the Mongolian pine plantations, water conditions and temperature were unfavorable for ECM growth in surface soil, but suitable in deeper soil. Therefore, it was concluded that the failure of natural regeneration in Mongolian pine plantations might be influenced by the lack of ECM in the surface soil because of high temperatures causing ECM death. Moreover, the majority of the root area is distributed in deeper soil, which alleviates the stresses on ECM development and arrests pine decline; this is beneficial for tree growth.     

白牦牛早期胚胎发育条件的初步研究

研究了卵母细胞质量和体外受精卵培养温度对白牦牛早期胚胎发育的影响。结果表明:白牦牛体外受精时要选择A级质量的卵母细胞,体外受精胚胎发育的最适温度为38℃。为完善白牦牛胚胎的体外生产体系提供了实验依据。     

Field evaluation of insect-resistant transgenic Populus nigra trees

The performance of insect-resistant transgenic poplar trees (Populusnigra) expressing a Cry1Ac gene from Bacillus thuringiensis subsp. Kurstaki HD-1 against poplar defoliators was evaluated in the field at the Manas Forest Station in Xinjiang Uygur Autonomous Region during1994–1997. The results showed that the average percentage of highly damaged leaves on the transgenic trees was 10% while that on the control trees in nearby plantations reached 80–90%. The average number of pupae per m² of soil at 20cm depth in transgenic poplar plantation was 18 which was only 20% of that found in the non-transgenic control field. The number of pupae and the leaf-damage on transgenic trees described above are all far below the threshold set for chemical protection measures. The non-transformed poplar trees grown in the same plantation with the transgenic trees were also protected indicating that cross protection occurred between these two kinds of trees. Insect-resistant transgenic poplar trees have a potential application value in afforestation. This revised version was published online in August 2006 with corrections to the Cover Date.     

Effect of stachyose supplementation on growth performance, nutrient digestibility and caecal fermentation characteristics in broilers

1. The objective of this study was to evaluate whether the oligosaccharide stachyose enhances gastrointestinal tract health by fermentation and proliferation of desirable bacteria species and thus affects growth performance and nutrient digestibility in broilers. 2. A total of 432 1-d-old male Arbor Acres (AA) broilers were randomly allocated to one of 6 treatments, with 12 replicate pens per treatment and 6 birds per pen. Chicks were fed a maize-hamlet protein 300 (HP300) basal diet with 0, 4.0, 8.0, 12.0 or 16.0 g/kg stachyose. A sixth diet contained no HP300 but soybean meal (SBM) and provided 8.7 g/kg stachyose and 3.1 g/kg raffinose. The duration of the study was 42 d. 3. Stachyose contents above 12.0 g/kg depressed group body weights, average daily gain and feed/gain but not feed intake during the whole experimental period. Broiler growth decreased linearly and quadratically with increasing stachyose content. No differences were detected between diets supplemented with 12.0 g/kg stachyose and SBM. 4. Nutrient digestibility tended to decrease but not significantly with increasing stachyose. 5. Stachyose content had no significant positive effects on caecal pH, microflora population and the resulting short-chain fatty acid (SCFA) metabolites during the 42 d experiment, with only butyrate differing significantly in the initial period.     

对CALIS中外文对照工具书101字段第一指示符取值的探讨

对CALIS中外文对照工具书的101字段第一指示符的取值情况进行了调研,对取值不规范、不统一的实例进行了归纳总结;结合编目工作实际,分析、探讨了中外文对照读物101字段第一指示符的取值原则。     

基于工程施工多领域竞争形势下的分包体系构建

随着建设规模的不断扩大,建筑施工企业在项目承接上呈现出多领域竞争的局面。项目中标份额不断增加,势必会引起施工力量需求的继续扩大,如何充分利用外部资源,解决企业自身劳动力短缺的问题,必定成为现在乃至今后建筑施工企业必须研究和解决的管理重要课题。笔者结合自身从事过的项目管理实际做法及经验,总结了水电工程项目分包体系和分包队伍核算体系,并综述了分包体系构建的背景、基本思路、具体做法以及实施效果等。