杨氏姬花蚤发生规律与防治的初步研究

研究并明确了吉林省白城向日葵杨氏姬花蚤(Mordellistena yangi Fan)的发生危害与生物学分类,以及该虫在当地的发生规律及主要生物学特性,提出以消灭越冬寄主,改进耕作管理措施为主,适时化学防治为辅的防治方法。     

华北酿酒葡萄气候区划指标的筛选与气候分区

 根据对华北地区96 个气象台站连续30 年气候资料的分析, 对华北的气候特点和葡萄适栽气候范围进行评估。对多种葡萄气候区划指标比较研究的结果表明: 生长期≥10 ℃的活动积温, 是华北地区酿酒葡萄气候区划的最适热量指标(一级指标) , 8～9 月水热系数(K值) 是适宜的二级指标。对分区标准进行适当调整后, 将包括河北、山西、内蒙古、北京、天津5 省(区) 、市在内的整个华北地区按一级指标划分为4 个气候区: 冷凉区、凉温区、中温区、暖温区。每一气候区又分别按K值分为极干燥亚区、干燥亚区、湿润亚区。对各气候区的葡萄和葡萄酒生产方向和适栽品种提出建议。     

噻呋酰胺240克，升悬浮剂防治花生白绢病田间药效试验

采用喷淋浇灌法开展了噻呋酰胺240克／升悬浮剂防治花生白绢病的田间药效试验。结果表明：噻呋酰胺240克／升悬浮剂对花生白绢病菌有较好的防治效果,药后14d,噻呋酰胺32、64mL／667m^2处理防效分别为49．4％和67．8％,明显优于生产上常用药剂异菌脲50％悬浮剂和多茵灵40％悬浮剂;噻呋酰胺32、64mL／667m^2处理收获后测产分别增产11．0％和17．8％,明显优于两对照药剂,且对花生生长安全,可在生产上推广应用。     

外源ABA对番茄花粉成熟的细胞学影响

花粉的成熟对植物的繁衍,尤其对农作物的产量和品质都有决定性的影响。花粉的成熟受极其复杂的激素网络调控,其中脱落酸是其重要的激素之一,而其对花粉成熟的调控机理尚不清楚。为探究脱落酸在花粉成熟过程中的作用,本文分别利用不同浓度的NDGA溶液和不同浓度的ABA溶液处理小孢子阶段的番茄雄蕊,于处理后的第2天、4天、6天及8天取样,利用显微镜观察花粉的细胞学形态。结果显示,不同浓度的NDGA和ABA处理均对番茄花粉的成熟产生显著影响,并不同程度地导致花粉畸形。其中,不同浓度的NDGA对花粉的影响不同,但相同处理时间内NDGA的浓度越大,异常花粉的比率越大;同一浓度下,随着处理时间的延长,异常花粉的比率增大。而不同浓度ABA溶液之间对番茄花粉的影响差异不明显,随着处理时间的延长,不同浓度ABA处理均使得异常花粉的比率增大。上述结果表明,ABA在番茄花粉成熟过程中起到重要的调控作用。     

清代中期花旗参的输入及影响

乾隆二十二年(1757)清朝政府实行"一口通商",只保留粤海关与西方国家海路的贸易职能,直至1842年南京条约的签订,广州成为西方国家进入中国的唯一通道和贸易场所。在此期间,花旗参是美国早期对华贸易的主要商品,在中美贸易中占有重要地位,并对中国社会生活产生重要影响。     

戊菌唑20%水乳剂田间防治草莓白粉病药效试验

为解决当前白粉病在草莓上危害日趋严重的问题,本研究以常用药剂苯醚甲环唑和三唑酮为对照药剂,在田间草莓上测试了戊菌唑20%水乳剂不同使用浓度下对草莓白粉病的防治效果。结果表明,戊菌唑20%水乳剂2个处理浓度的防效显著高于对照药剂苯醚甲环唑10%水分散粒剂处理的防效,且极显著的高于对照药剂三唑酮15%可湿性粉剂处理的防效。     

谈果树栽培相关问题

随着国家退耕还林力度和政策资金投入加大,群众退耕还林积极性极大提高。结合实际的工作经验,谈谈关于果树栽培的技术及问题。     

Identification of porcine serum proteins modified in response to HP-PRRSV HuN4 infection by two-dimensional differential gel electrophoresis

Since 2006, highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has become the major pathogen attributed to the prevalent porcine reproductive and respiratory syndrome (PRRS) in China. The present study aims to identify serum proteins modified in response to infection of HuN4, a HP-PRRSV strain isolated from a farm in 2006. 2-D DIGE analysis allowed for the detection of 19 differentially expressed protein spots, of which 18 were identified by MALDI-TOF/TOF MS. These 18 spots represented for a total of 9 proteins (6 up-regulated and 3 down-regulated), most of which belonged to the acute phase proteins in swine and showed a trend of regression in the late phase of the experiment. One of a series of AGP spots was identified for the first time to be decreased in acute phase of PRRSV infection in swine. But the whole level of the protein in the serum did not show significant changes by Western blot. The rising tendency of Hp was confirmed by Western blot and ELISA. These altered proteins were probably involved in the inflammatory process triggered by HuN4 and in alleviating the oxidative damage occurring in the process. In summary, these results may provide new insights into understanding the mechanisms of HP-PRRSV infection.     

Highly pathogenic porcine reproductive and respiratory syndrome virus GP5 B antigenic region is not a neutralizing antigenic region

In 2006, highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) caused great economic losses emerged in China and continues to be a threat for the pig industry. B antigenic region (AR) ((37)SHL/FQLIYNL(45)) of GP5 was considered to be a major linear neutralizing AR in PRRSV classical strains. However, peptide-purified antibodies against this AR did not neutralize PRRSV in a recent report. Compared with classical PRRSV, one amino acid mutation (L/F(39)→I(39)) was found in B AR of HP-PRRSV. To study the ability of B AR of HP-PRRSV to induce neutralizing antibody (NA) in vitro and in vivo, rabbit antisera against B AR with and without the mutation and pig hyperimmune sera with high titer of NAs against HP-PRRSV were prepared. Immunofluorescence assays (IFA) showed that the two rabbit antisera both had reactivity to classical PRRSV CH-1a and HP-PRRSV HuN4 with no observable difference in IFA titer. However, antisera did not have neutralizing activity against classical PRRSV CH-1a and HP-PRRSV HuN4. No correlation was observed between the levels of anti-B AR peptide antibodies and NAs in pig hyperimmune sera that were detected by indirect ELISA and virus neutralization, respectively. B AR peptide-specific serum antibodies had no neutralizing activity and, GST-B fusion protein could not inhibit neutralization of NAs in pig hyperimmune sera. Based on these findings, we conclude that B AR of HP-PRRSV is not a neutralizing AR of HP-PRRSV GP5.     

大庆农村区域休闲产业的发展方向和模式研究

大庆市进入工业化后期，休闲产业将成为促进经济发展的主导产业。基于对大庆休闲产业发展现状的调研和分析，进一步揭示大庆农村区域发展休闲产业的优势，根据大庆市休闲产业发展的目标定位，构建农村区域特色组合休闲产品供给体系，形成环城休闲游憩带，成为重点发展方向，确定发展模式是农村区域休闲产品组合型和资源组合平台型。