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991.
AIM: To explore the feasibility and biological characterization of long-term regulated expansion of JAK2 transduced human CD34+ cord blood cells in vitro.METHODS: A retrovirus (RV) vector which contains JAK2 catalytic domain and two binding sites for a chemical inducer,dimerization (AP20187),was cloned (designated MGI-F2JAK2).CD34+cells were enriched from cord blood with a MiniMACS system.The purified CD34+ cells were transfected with supernatant from the retrovirus packaging cell line that expressed JAK2.Following transduction,cells were expanded into four groups: AP20187 alone,FL alone,TPO,alone,AP20187+FL+TPO,respectively.The expanded cells were monitored by GFP expression,immunophenotyping,progenitor colony assay,karyotype analysis as well as tumorigenesis in nude mice.RESULTS: The purity of selected CD34+ cells was over 91% and gene transfer rate was 49.32%±6.21%.Only the group of AP20187 +FL+ TPO was obtained a significant sustained outgrowth of the transduced CD34+ cord blood cells.The percentage of GFP+ cells consistently produced a rise to the 90% peak level by the end of 8th week of culture.Flow cytometry analysis showed that the phenotype of the expanded cells was CD33+,CD61+ and Gly-A+ partial positive;CD38+ and HLA-DR+ strong positive,while CD2,CD7 and CD19 were almost negative.Colony assays performed in methycelluos,which can give rise to BFU-E,CFU-GM and CFU-Mix,the CFU-GM was predominantly in all colonies.The tumor was not observed in nude mice and the karyotype analysis was normal from expanded cells.CONCLUSION: The results demonstrate that AP20187-mediated activation of JAK2 signaling is capable of stimulating expansion JAK2 transduced CB CD34+ cells in combination with FL and TPO.This system may have applications for studies in signaling transduction,hematopoiesis,and for gene and cell therapy. 相似文献
992.
AIM: To investigate the preventive effects of Shengmai San (SMS) on oxidative damage in mentally stressed mice.METHODS: An oxidative stress mouse model was established by moustache-removed. Protein carbonyl and thiobarbituric acid reactive substance (TBARS) formation were determined as the oxidative stress markers.RESULTS: (1)Moustache-cut was founded to significantly enhance the behavioral movements of mice, especially large movements (movement 2 and rearing). SMS pre-administration inhibited the accelerated movements. (2) Protein carbonyl was increased in brain, heart, liver and kidney. TBARS in liver and heart increased in the moustache-cut mice, but SMS pretreatment inhibited the increased protein carbonyl and TBARS.CONCLUSION: SMS has the preventive effects on oxidative damage induced by emotional stress. 相似文献
993.
AIM:To investigate the role of caspase 3 inhibitor Ac-DEVD-CHO in caspase 3 signaling pathway and NF-κB activation induced by 10-hydroxycamptothecin (HCPT) in human breast carcinoma cells. METHODS:The cell growth inhibition was measured by MTT assay. Agarose gel electrophoresis was performed for detecting cell apoptosis. Western blotting was used for determining protein expression. DIG-EMSA was conducted to measure the DNA-binding activation of NF-κB. RESULTS:Caspase 3 inhibitor Ac-DEVD-CHO attenuated HCPT-induced apoptosis in human breast carcinoma. Ac-DEVD-CHO also suppressed the degradation of caspase 3 and IκBα,and arrested the activation of NF-κB. CONCLUSION:Caspase 3 inhibitor Ac-DEVD-CHO regulates the activation of caspase 3 and NF-κB,and attenuates apoptosis in Bcap37 cell line induced by HCPT. 相似文献
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996.
^3H—BA不同溶解法对脐橙果实吸收^3H—BA的影响 总被引:1,自引:0,他引:1
^3H-BA分别采用普通热酒精溶解法,加PT39助溶剂的改进溶解法配成^3H-BA+GA3,于“清家”脐橙第一次重量落果高峰前涂果,改进溶解法使脐橙幼果在24小时吸收^3H-BA达22388dpm,果内^3H-BA浓度达0.88×10^-6,而普通热酒精溶解法仅分别为12890dmp和0.45×10^-6,无论采用哪种方法溶解^3H-BA脐橙幼果对^3H-BA的吸收速度都是果后24小时内最快,此后 相似文献
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998.
机动车污染排放主要是由车辆在道路中的行驶造成的,机动车在拥堵情况下所造成的污染排放会明显高于正常行驶状况。文中运用社会学调查抽样的方法,通过搜集大量的出租车票据对北京市目前道路车辆的行驶速度进行了调查,结果表明北京市道路车辆行驶速度普遍偏低。同时,通过对北京市5个交通污染监控点的污染物排放实时数据的分析,得出行驶速度和交通污染排放的相关关系,运用MOVEs模型核算了提高车辆行驶速度所带来的减排效果,可以借鉴国际发达国家和城市的经验,通过采取缓解交通拥堵的方式来有效控制路上移动源的排放。 相似文献
999.
近现代陇东地方居住形态主要表现出两种形式:一是散居型,住民远离河川、大道,遁隐于偏僻之地;二是聚居型,聚族居住,建堡自卫。近现代陇东社会所呈现出的这种居住形态不仅受地方自然条件、经济社会条件的影响,安全防卫、抵御劫掠的安全需要同样深刻影响着近代陇东地方的居住形态。随着社会由乱而治,住民逐渐搬出深山,向河川、阔塬等水、路、地条件较好的地方集中,窨子、堡子等防卫性建筑因丧失使用价值而遭废弃,逐渐淡出了人们的日常生活实践。 相似文献
1000.