Mechanism of mesenteric lymph reperfusion aggravates multiple organ injury in superior mesenteric artery occlusion shock rats

AIM: To explore the mechanism of mesenteric lymph reperfusion (MLR) aggravates multiple organ injury in superior mesenteric artery occlusion (SMAO) shock rats. METHODS: Male Wistar rats were randomly divided into 4 groups: in sham group, only anesthetization and operation were performed; in MLR group, occlusion of mesenteric lymphatics (ML) for 1 h followed by 2 h of reperfusion; in SMAO group, occlusion of superior mesenteric artery (SMA) for 1 h followed by 2 h reperfusion; in MLR+SMAO group, occlusion of SMA and ML for 1 h followed by 2 h of reperfusion. The homogenates of liver, kidney, myocardium and lung were prepared for determining the activities of free radical, nitric oxide, myeloperoxidase (MPO) and cell membrane ATPase. RESULTS: The MDA, NO contents and NOS, MPO activities of multiple organic homogenate in SMAO and MLR+SMAO group were higher than those in sham and MLR group, and these indexes in MLR+SMAO were increased significantly than those in SMAO group. The SOD and ATPase activities of muliple organic homogenate in SMAO and MLR+SMAO group were lower than those in sham and MLR group, and those in MLR+SMAO group was decreased obviously than those in SMAO group. CONCLUSION: The MLR enhances the multiple organ free radical injury, NO synthesis and release, PMN detention and decreases the activity of cell membrane ATPase, aggravating the major organs injury in SMAO shock rats. Intestinal lymphatic pathway plays an important role in the pathogenesis of SMAO shock.     

Regulatory mechanism of sodium-calcium exchanger in hypertrophic myocardium

Myocardium hypertrophy is a compensated response to cardiac disease in which intracellular Ca2+ concentration is elevated, the essential signal that results in hypertrophy. The cardiac sodium-calcium exchanger (NCX) is a bidirectional ion transporter in sarcolemma. The decrease in NCX activity as well as its inverse mode is one of the main mechanisms that contribute to cytosolic Ca2+ loading in hypertrophy. The extracellular signal molecules, the change of Na+ /Ca2+ concentrations and intracellular modulating proteins during cardiac hypertrophy can affect the activity or NCX function, and increase intracellular Ca2+. We summarized the regulatory mechanism of NCX in myocardium hypertrophy.     

Prokaryotic expression and identification of a small peptide of osteopontin

AIM: To express osteopontin 13 peptide (OPN 13) in E.coli, and to test the biological activity of the purified products. METHODS: cDNA fragments containing RGD sequences were cloned into prokaryotic expression vector pET-32c(+) including His coding sequence to construct pET-32c-OPN 13 plasmid. E.coli DH5α transformed by pET-32c-OPN 13 plasmid was induced by IPTG at different concentrations for different times to identify the optimal induction condition. Expressed His-OPN 13 fusion protein was purified via Ni-NTA His Bind Resin metal chelation chromatography, and detected by VSMCs adhesion and migration analysis. RESULTS: His-OPN 13 fusion protein was expressed in soluble manner. The fusion proteins were purified via Ni-NTA His Bind Resin affinity chromatography. His-OPN 13 fusion protein specifically inhibited adhesion and migration of VSMCs stimulated by osteopontin in dose-dependent manner. CONCLUSION: The OPN 13 peptide is successfully expressed in E.coli DH5α. The purified His-OPN 13 fusion protein could inhibit the adhesion and migration of VSMCs stimulated by osteopontin.     

富士苹果AFLP体系的优化及其在鉴定早熟芽变中的应用

 以‘长富2号’苹果为材料,采用优化的AFLP技术对其早熟芽变与母株进行初步研究,筛选了64对引物,其中43对引物扩增结果理想,共产生了2,700条带。有4对引物在芽变与母株之间产生了7条差异片段,主要集中在350~800 bp之间。研究表明早熟芽变是由于遗传物质改变造成的,同时也说明利用该体系对苹果芽变进行分析和鉴定获得成功。     

褐蘑菇子实体活性多肽提取工艺

采用单因素和正交试验,以水提醇沉的方法来获取褐蘑菇子实体中的活性肽类物质。且根据不同条件下活性多肽的提取量为指标来确定活性多肽的最佳提取条件（料水比1：12、时间6h、温度4℃、pH值7、水醇比1：1．5）,旨在为褐蘑菇子实体中蛋白质的开发应用打下基础。     

试论景洪热带地区园林植物造景的特点

对景洪热带地区独有的少数民族,结合当地自然风貌,尝试将景洪热带地区总结出人与大自然植物景观和谐相处,重视"意"与"匠"的结合,傣族民间园林植物造景与园林其它要素的结合,公园的植物造景特色,重视热带景观的营造5个植物造景的特点,对其进行了分析,并对热带地区园林植物造景研究的意义提出看法.     

不同基质及生根剂浓度对五种园林植物扦插生根的影响

以5种园林植物为材料,进行不同基质与不同生根剂浓度对植物扦插生根的比较研究.结果表明:月季、大叶黄杨最适宜的基质是河沙,小叶女贞、八角金盘最适宜的基质是珍珠岩,鹅掌柴最适宜的基质是混合基质.月季、大叶黄杨最合适的生根剂浓度为3 000 mg/kg,小叶女贞最合适的生根剂浓度为5000mg/kg,八角金盘、鹅掌柴最合适的生根剂浓度为1000mg/kg.     

水杨酸与高温锻炼对高温胁迫下葡萄叶肉细胞质膜ATPase活性的影响

采用水性两相分配法对经水杨酸(SA)和高温锻炼处理过的葡萄幼苗叶肉细胞中ATPase进行了分离和测定.结果表明:高温下,细胞质膜ATPase活性随着胁迫程度的加深均呈下降趋势,经SA和高温锻炼预处理后再经高温胁迫,发现质膜ATPase的活性低于正常条件下的水平(CK1),但明显高于未经处理直接进行高温胁迫后ATPase活性水平,即高温锻炼和SA预处理的质膜酶活仍保持了较高的活性,并且后一种处理效果更明显.说明质膜上ATPase活性的变化对高温胁迫作出了一定的响应,SA和高温锻炼对提高葡萄幼苗叶片的抗热性与二者可以维持细胞质膜上ATPase活性的稳定有关.     

干旱胁迫下外源山梨醇对平邑甜茶耐旱性影响

测定了干旱胁迫下外源喷施50 mg/L和100 mg/L山梨醇后平邑甜茶叶片光合特性、叶片水势和新梢生长速率.结果表明:叶面喷施山梨醇可以改善受胁迫植株的光合状况,减缓叶片水势下降,减轻新梢生长受抑,提高植株耐旱性.