从进口冻鸡爪中检出鸡白痢沙门氏菌

2001年12月,南海某公司从美国进口一批冻鸡爪,我局按规定进行抽样检验,经细菌分离培养和生化试验,确定该批货物含鸡白痢沙门氏菌.     

Bio—G除臭剂对蛋鸡舍内氨气及产蛋性能的影响

在面积相等，饲养密度相近的2栋蛋鸡舍内连续15d喷洒Bio-G除臭剂，每天1次，每次5L，结果表明，喷洒Bio-G后，鸡舍氨气含量明显下降，但对蛋鸡产蛋性能无明显影响。     

A Preliminary Comparison of Lidocaine and Xylazine as Epidural Analgesics in Ponies

Xylazine (0.35 mg/kg) or lidocaine (0.35 mg/kg) was injected into the epidural space of six ponies to compare their effectiveness as epidural analgesics. Each pony received both treatments at 1 week intervals with the order of treatments randomized. Xylazine produced analgesia of significantly longer duration (247 +/- 58 minutes) than that produced by an equal dose of lidocaine (135 +/- 22 minutes). Mild transient ataxia of no clinical significance developed in all ponies with both treatments. Spinal cords were removed from two ponies and examined histologically. No discernible pathologic changes were noted.     

Immune responses and protective efficacy of a recombinant swinepox virus co-expressing HA1 genes of H3N2 and H1N1 swine influenza virus in mice and pigs

The recombinant swine poxvirus rSPV/H3-2A-H1 co-expressing HA1 genes of H3N2 and H1N1 subtype SIV has been constructed and identified. Inoculations of rSPV/H3-2A-H1 yielded ELISA and neutralization antibodies against SIV H1N1 and H3N2, and elicited potent H1N1 and H3N2 SIV-specific INF-γ response from T-lymphocytes in mice and pigs in this study. Complete protection against SIV H1N1 or H3N2 challenge in pigs was observed.     

“黄白散”对兔接种RHD疫苗后免疫功能的影响

在注射兔病毒性出血症 (RHD)疫苗后 ,30只兔随机分为 2组 ,试验组兔饲喂含 1 5 %黄白散的饲料。从接种到第 1 80天 ,每组取兔 8只 ,每隔 1 5d采血 1次 ,检测血凝抑制 (HI)抗体 ;在接种前 1天及接种后 5、 1 0、 1 7、 2 4、 31、 41、 5 1d ,每组取兔 1 0只 ,采血检测ANAE+淋巴细胞百分率 ;在 1 80、 2 4 0、 30 0d时 ,每组分别取兔 5只做攻毒保护试验。结果表明 :2组兔HI抗体峰值分别为 7 3log2和 9 8log2 ,差异极显著 (P <0 0 1 ) ;试验组疫苗保护期可延长 60d ;试验组ANAE+率从第 1 0天至 5 1天与对照组比较 ,差异极显著(P<0 0 1 )。所以黄白散可增强兔接种RHD疫苗后的特异性免疫功能 ,增强RHD疫苗的免疫效果。     

兔病毒性出血症首免时间的研究

针对生产上出现的从断奶育成这一年龄段多发兔病毒性出血症的实际情况，采用快速、敏感的Dot-ELISA方法，通过测定不同免疫状态下的母兔所产兔的母源抗体动态变化规律，并进一步测定不同抗体效价的成年兔攻毒后是否得到保护的结果，再结合生产实际，得出兔病毒性出血症首免时间为30日龄，且剂量加倍。     

Pyruvate kinase is necessary for Brucella abortus full virulence in BALB/c mouse

Brucellosis, caused by a facultative intracellular pathogen Brucella, is one of the most prevalent zoonosis worldwide. Host infection relies on several uncanonical virulence factors. A recent research hotpot is the links between carbon metabolism and bacterial virulence. In this study, we found that a carbon metabolism-related pyruvate kinase (Pyk) encoded by pyk gene (locus tag BAB_RS24320) was associated with Brucella virulence. Determination of bacterial growth curves and resistance to environmental stress factors showed that Pyk plays an important role in B. abortus growth, especially under the conditions of nutrition deprivation, and resistance to oxidative stress. Additionally, cell infection assay showed that Pyk is necessary for B. abortus survival and evading fusion with lysosomes within RAW264.7 cells. Moreover, animal experiments exhibited that the Pyk deletion significantly reduced B. abortus virulence in a mouse infection model. Our results elucidated the role of the Pyk in B. abortus virulence and provided information for further investigation of Brucella virulence associated carbon metabolism.     

应用兔肾原代细胞培养 兔粘液瘤病毒的研究

试用兔肾原代细胞传代；增殖兔粘液瘤病毒，呈现典型ＣＰＥ。粘液瘤病毒ＳＧ３３株在兔肾细胞上连续传３代之后，就不产生ＣＰＥ，在鸡胚上传３代再回归兔肾原代细胞，又呈现ＣＰＥ。ＳＧ＿（３３）在鸡胚上不能产生明显痘斑。维持液ｐＨ值影响ＳＧ＿（３３）在兔肾原代细胞上形成ＣＰＥ。     

桑叶超氧物歧化酶（SOD）的研究

分别用高速冷冻离心法和低速室温离心法提取了桐乡青、湖桑１９７、团头荷叶白、尖头荷叶白４个品种的顶芽、第５位和第１０位叶以及蚕沙残桑叶的ＳＯＤ粗酶液，然后用ＮＢＴ光还原法和聚丙烯酰胺凝胶电泳分别分析测定了它们的活性及其同工酶。试验结果表明：用高速冷冻离心法和低速室温离心法提取的ＳＯＤ，其活性无显著差异；同一叶位的桑叶ＳＯＤ活性在不同桑品种之间无显著差异；同一桑品种不同叶位的ＳＯＤ活性有显著差异；蚕沙残桑叶的ＳＯＤ活性与第１０位叶的ＳＯＤ活性相当。发现桑叶中有３～４种ＳＯＤ同工酶。     

山黧豆低毒品种的筛选及栽培利用技术研究

对国内外引进的６５个山黧豆品种，经３年观察和农艺性状的测定以及β－草酰氨基丙酸（ＢＯＡＡ）、营养成分的分析，筛选出产量，蛋白质含量高，毒素含量低，经毒理学实验为安全饲用的黑龙江山黧豆－１，黑龙江山黧豆－２，西德山黧豆３个品种供生产中应用，还针对山黧豆作为饲草（料）推广应用中的播种量，磷肥施用量，压青及根茬肥效等栽培利用措施进行了探讨。