Effects of Medium-chain Triglyceride on Growth Performance and Serum Biochemical Indices in Broilers

The study was conducted to investigate the effects of medium-chain triglyceride replacing the soybean oil in the basal diet on growth performance and serum biochemical indices of broilers.One hundred and forty four healthy,similar body weights AA broilers at the age of 14 days were randomly assigned to four groups with six replicates ger group and six broilers per replicate.Groups A,B,C and D were respectively fed with 0,0.6%,1.2% and 2% medium-chain triglyceride substituted the equivalent amount of soybean oil (using corn flour to balance the energy levels in each group) .The test lasted for 28 days and the indexes were measured once every two weeks.The results showed that the average daily gain and average daily feed intake of groups B,C and D were higher than that of group A,and the F/G was lower than group A.The average daily gain of groups C and D at 28 to 42 days old and group D at 14 to 42 days old were significantly higher than that of group A (P < 0.05).The serum glucose,total content of amino acid,total protein and albumin of groups B,C and D at age of 28 and 42 days old were higher than that of group A,while the serum triglyceride,total cholesterol and urea nitrogen levels were lower.The total cholesterol content at 42 days old and triglyceride concentrations at 28 days old of group C were significantly lower than those of group A (P < 0.05).The results indicated that medium-chain triglyceride could raise average daily gain and reduce the total cholesterol content and triglyceride concentrations of broilers,which were useful for improving the growth performance.     

产碳青霉烯酶肠杆菌科细菌检测方法研究进展

抗生素的不合理使用使得细菌耐药性快速产生及传播，细菌耐药性已然成为重大公共卫生问题。碳青霉烯类抗生素在临床治疗中表现良好，常作为治疗多重耐药菌感染的最后一道防线，碳青霉烯耐药菌的出现对公共卫生安全产生了极大的威胁。产碳青霉烯酶是碳青霉烯耐药菌耐药的主要机制，因此，对产碳青霉烯酶肠杆菌科细菌的检测至关重要。目前，对于碳青霉烯酶的检测手段以表型检测和分子生物学检测方法为主。表型检测一般易于操作，成本低廉，便于在临床进行，但在检测效率和检测用时方面差强人意。分子生物学及其他新型技术在保持高特异性和敏感性的基础上能做到快速、高通量检测，但试验检测的成本高，需专业设备和人员，部分技术在碳青霉烯酶检测领域仍有待开发。当前，多种碳青霉烯酶的检测方法可针对不同的需求和目的，并没有一项检测能满足所有情况。进行检测时，试验所需时间、操作难度、经济成本等都需列入考量，应根据实际情况选择合适的检测方法。笔者通过结合当前背景，归纳总结产碳青霉烯酶肠杆菌科细菌的检测方法，从试验特异性与敏感性、可操作性、检测时间、试验成本等方面分析比较各个方法特点，以期为之后新的检测方法的开发及现有方法的改良研究提供切入点，为临床监测与检测方案的制定和实行提供思路。     

Expression,Purification and Activity Evaluation of Mb1230 Gene of Mycobacterium bovis

To explore the value of Mb1230 protein of Mycobacterium bovis in the diagnosis of bovine tuberculosis,we obtained the Mb1230 gene by PCR and constructed recombinant plasmid pET-22b-Mb1230.Recombinant Mb1230 protein was obtained by IPTG induction and purified by affinity chromatography.The activity of the recombinant protein was evaluated by TST test,IGRA test and indirect ELISA.The size of the recombinant protein matched with the theoretical value proved by SDS-PAGE;Western blotting result showed that the recombinant protein could react with mouse anti-His antibody,and had specific band;The results of TST test,IGRA test and indirect ELISA test also showed the recombinant protein had antigenic activity.The results indicated the recombinant protein Mb1230 had good B cell and T cell activity,so,it had the potential application in the diagnosis of bovine tuberculosis.     

智能机械通风控制系统的实现和应用

系统采用计算机网络控制技术、智能技术,自动控制粮仓通风设备启停和粮仓门窗的开关。依据《储粮机械通风技术规程》和相关智能通风策略对粮仓进行通风,达到降温通风、降水通风和调质通风等目的,有效控制粮仓的储粮环境和粮食品质,从而达到绿色安全储粮的目的。     

实施品牌战略做大做强茶产业

宁都县现有茶园1 466.67 hm2,产值5 000多万元.笔者从政策扶持、名优茶发展、创新机制等措施以促进产业发展提出思路.     

甘蔗引进新品种(系)的试验表现及评价

为筛选出高产、高糖、综合性状表现较好的甘蔗新品种充实蔗区,对17个国内外甘蔗引进新品种(系)进行了1年新植1年宿根种性比较试验。结果表明,平均蔗茎产量显著高于新台糖22号的参试品种有SP80-3280、K86-110、ISD24、FR93-344、CP84-1591、VMC88-354和K82-129,增产10.54%~21.47%。平均蔗糖分含量高于新台糖22号的参试品种有VMC71-39、CP94-1100、FR93-435和云蔗03-258,蔗糖分含量提高0.2~1.4个百分点。     

腐殖酸与EDTA对高铁酸钾降解水中孔雀石绿影响的研究

在高铁酸钾与孔雀石绿的质量比为10∶1,pH值为7.0的条件下,考察腐殖酸与EDTA对高铁酸钾降解水体中孔雀石绿的影响。结果表明,加入少量的腐殖酸和EDTA溶液,孔雀石绿溶液的吸光度降低,有利于高铁酸钾对孔雀石绿的降解;EDTA的加入量过多时,反而抑制高铁酸钾对孔雀石绿的降解;加入2.0 mL腐殖酸和4.0 mL EDTA溶液降解2 h效果较好。     

民族旅游地民居客栈经营的关键因素研究——以世界遗产地丽江古城为例

旅游体验满意是衡量旅游者忠诚与重顾的关键因素。旅游者对以世界文化遗产地丽江古城的民居客栈体验的网络评价，主要涉及了“家”、整洁、舒适、环境、房间陈设、位置等六个方面，这些都是影响民居客栈成功经营的关键因素。但是就旅游者体验来说，这些因素明显缺乏了地方文化的展现与创新。基于此，文章从酒店化与专业化、家庭化与生活化、文化化与乡野化的视域上重新设计了民居客栈经营发展的创新模式，凸显出民居客栈产品与酒店产品的差异性，创建一种独具地方特色的民居品牌，助推民居经营的永续发展。     

胶莱盆地北部风尘堆积物光释光年代序列研究

山东省胶莱盆地的高岗部位存在大量的风尘堆积物,对其年代的研究一直存在较大的分歧.对胶莱盆地的潍坊前埠下风尘堆积剖面进行了系统的光释光年代测试,并与其以北16.9km的昌邑县徐林庄剖面的年代进行了对比,初步建立了胶莱盆地风尘堆积的光释光年代序列,研究表明该区风尘堆积主要是晚第四纪以来形成的堆积物.     

乌鲁木齐市空气污染成因及防治对策探讨

分析了乌鲁木齐市1999～2009年间每年以及2005～2007年各月份空气环境质量中主要污染物SO2、NO2、PM10的监测结果 ;探讨了造成该市空气污染的自然条件、城市功能布局、能源结构、供热点分布、移动源、监测和治理能力等因素,并从改善生态环境、优化工业布局、加强污染治理、调整能源结构、推进节能工作、防治机动车尾气排放、提高监测能力及公众意识等方面提出了污染防治的对策和建议。