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51.
台湾青枣不同品种花粉萌发和生活力测定   总被引:12,自引:0,他引:12  
以5个台湾青枣品种盛花期花粉为试材,应用正交试验测定不同品种花粉萌发率及适宜的萌发条件,并用4种不同方法测定花粉生活力。结果表明:(1)不同品种的花粉萌发率存在显著差异,其中大世界的花粉萌发率最高。(2)硼酸浓度、培养温度等因素对花粉萌发率有极显著影响。最适培养基为含琼脂1%、蔗糖15%、硼酸0.02%的组合,适宜温度为30—32℃。(3)花粉生活力的测定中,离体萌发法是最佳选择,I—KI染色法、醋酸洋红染色法和TTC染色法不适宜用作台湾青枣花粉生活力的测定。  相似文献   
52.
在室内人工模拟干旱条件,测定了干旱胁迫下披碱草和野大麦及其杂种幼苗的相对含水量、细胞膜透性、游离脯氨酸含量的变化。结果表明,干旱胁迫下亲本披碱草幼苗的组织相对含水量下降的幅度最低,细胞膜受害最轻,游离脯氨酸积累的最多,相对生长率最高,表现出很强的抗旱性。亲本野大麦幼苗的组织相对含水量下降的幅度最大,细胞膜受害最重,游离脯氨酸积累的最少,相对生长率最低,抗旱性弱。杂种F1幼苗的保水能力、细胞膜受害程度和游离脯氨酸积累能力、相对生长率均介于双亲之间,亦即其抗旱能力大于野大麦而小于披碱草,表现为双亲的中间型。正、反交对杂种F1的抗旱能力没有明显影响。  相似文献   
53.
从考察我国高等学校法律地位的理论与现状入手,分析了高等学校在法律地位上存在的问题,探讨了高等学校的教育权,认为:当前我国高等学校的法律地位主要取决于高校自身所具有的多种社会职能,以及由多种社会职能所必然带来的法律特征.  相似文献   
54.
试验将槐花蜂花粉进行振动磨超微粉碎破壁,分别按1 g/kg、5 g/kg、10 g/kg三个不同剂量混到蛋鸡饲料中,从7日龄开始饲喂至50日龄,每个剂量分两个试验,试验一用新城疫Ⅳ系疫苗免疫雏鸡,利用β-微量法监测血清中的血凝抑制抗体效价的动态变化;试验二用大肠杆菌油乳苗免疫雏鸡,利用间接血凝法监测血清中大肠杆菌抗体效价的动态变化。分别定期称重捕杀取脾、胸腺、法氏囊,计算免疫器官指数,取肠道内容物计数大肠杆菌和乳酸菌,测定肠道内pH值。结果表明,不同剂量的破壁蜂花粉均能不同程度地提高病毒抗原性抗体效价和菌体抗原性抗体效价,对雏鸡免疫器官的发育以及肠道内环境、菌群的平衡也有一定影响。  相似文献   
55.
新疆石河子地区沙漠增温效应对绿洲农业影响的研究   总被引:2,自引:1,他引:2  
由于沙漠 ,绿洲 ,空气的比热等热性能不同 ,形成沙漠夏季温度明显高于绿洲 ,进而对绿洲地区增温 ,称沙漠增温效应。石河子地区 1 0 0 km范围内沙漠对绿洲的增温平均 1 .76℃ ( 1~ 2℃ ) ;根据绿洲距沙漠的远近与气温间存在的线性关系 ,回归方程为 :y=2 7.5 2 97- 0 .0 335 x。 r=- 0 .881 3*。沙漠增温效应对绿洲农业有多方面作用 ,形成夏季富照高温、秋季大日较差以及促成棉花在夏季的“冷害”等 ,深刻地影响绿洲特色农业、栽培技术及人们生活  相似文献   
56.
AIM:To detect the association between the polymorphism of Fc receptor γ chain gene at position-29 in promoter and systemic lupus erythematosus(SLE).METHODS:The genotypes at position -29 in promoter of Fc receptor γ chain gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 180 patients with SLE and 140 ethnically matched controls in southern China.RESULTS:The frequencies of TT genotype(33.3%) and T allele (54.4%) at position -29 in patients with SLE were significantly higher than those in controls (17.2% and 42.9%, respectively), whereas, the frequencies of GG genotype (24.4%) and G allele (45.6%) in patients with SLE were remarkably lower than those in controls (31.4% and 57.1%, respectively) (P<0.05). The TT genotype and T allele at position -29 were not associated with lupus nephritis in SLE patients (P>0.05).CONCLUSION:Our results indicate that the T allele at position -29 in promoter of Fc receptor gene probably contributes to the susceptibility to SLE, but does not play a role in the occurrence of lupus nephritis.  相似文献   
57.
AIM:To investigate multi-potential of rat bone marrow mesenchymal stem cells (rBMMSC) and mutation inclination, the rBMMSC were long passaged in vitro. METHODS:Cellular cycles of different passages were assayed by FACSan flow cytometry and karyotypes of passage 6, passage 25 and passage 45 were compared by G-binding analysis. RESULTS:The early passages and long-term passages all showed strong proliferation; passage 6, passage 25 and passage 45 all showed normal karyotype. CONCLUSION:Long-term culture and passage of rBMMSC still remains strong proliferation. With this capability, the mutation inclination is not enhanced.  相似文献   
58.
AIM:To study influence of ischemia-reperfusion(IR) on apoptosis and expression of apoptosis-related genes Fas-L, Bax and Bcl-2 of sinoatrial node(SAN) cells in rabbits in vivo. METHODS:Ninety healthy adult rabbits were divided randomly into control group, ischemia groups (I10 min, I30 min, I60 min and I120 min) and IR groups (I10 minR4h, I30 minR4h, I60 minR4hand I120 minR4h). IR injury model of SAN was established by occluding and loosening the start section of right coronary artery. The apoptosis of SAN cells was detected by TUNEL staining. The expression of Fas-L, Bax and Bcl-2 of SAN cells was detected by immunohistochemistry. RESULTS:①No obvious apoptosis of SAN cells was observed in control group, I10 min and I30 min groups. Apoptosis of different degrees in SAN cells were found in 68.3%(41/60) rabbits in I60 min, I120 min and 4 subgroups of IR. ②The highest expression of Fas-L and Bax was observed in I120 min group and that of Bcl-2 was in I60 min group. ③The highest expression of Fas-L and Bax was observed in I60 minR4h group. The peak level of Bcl-2 was observed in I30 minR4h group. ④The expression of Fas-L and Bax was significant higher in IR group than that in ischemic group at the same time point. CONCLUSION:Ischemia and IR induced apoptosis of SAN cells in rabbit in vivo. Fas-L、Bax、Bcl-2 may participate in the regulation of apoptosis and the injury during IR aggravates the apoptosis of SAN cells.  相似文献   
59.
AIM:To study the effect of TGF-β1 and TNF-α antisense PS-ODNS on ex vivo expansion of hematopoietic stem/progenitor cells (HSPC). METHODS:CD34+cells were purified from fresh umbilical cord blood by immunomagnetic beads, and mononuclear cells were purified from bone marrow by Ficoll-hypaque. The effects of TGF-β1 and /or TNF-α antisense PS-ODNS on ex vivo expansion of CD34+ cells、CFU-GEMM、CFU-GM、CFU-E and BFU-E were detected by using liquid and semi-solid culture systems.RESULTS:TGF-β1 antisense PS-ODNS cooperated with cytokines increased the number of CD34+ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E, which was as 4, 2.6, 2.7, 1.8, 2.1 times as that of the control (the cytokines combination), respectively. TNF-α antisense PS-ODNS cooperated with cytokines respectively increased the number of CD34+ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E by 4, 2.9, 2.6, 1.7, 1.8 times as that of the control. The above two antisense PS-ODNS cooperated with cytokines could respectively increased the number of CD34+ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E by 5.3, 2.1, 2.7, 1.9, 1.8 times as that of the control.CONCLUSION:Inhibition of endogenous TGF-β1 and TNF-α by antisense PS-ODNS will be one of the effective methods to expand HSPC ex vivo.  相似文献   
60.
AIM:To study the effect of cilazapril on pulmonary vascular endothelial dysfunction in hypoxic rats. METHODS:The structure and function of endothelium in hypoxic rats were studied by biochemical analysis, radioimmunoassay, transmission electron microscope and correlated with hemodynamic. RESULTS:1) The change and damage of ultrastructure in endothelial cell (EC) were obsevered in hypoxic rats. 2) The contents of plasma nitric oxide (NO) and superoxide dismutase (SOD) activity in blood as well as endothelial nitric oxide synthase (eNOS) activity in the lung tissue were significantly lower in the hypoxic rat than those in contral animals. The concentrations of plasma endothelin-1(ET-1) and angiotensin converting enzyme(ACE) as well as malondialdehyde(MDA) were significantly higher in the hypoxic rat than these in contral animals. The relaxing and contracting factors had a significant positive/negative correlation with mean pulmonary artery pressure (mPAP). 3) Cilazapril significantly decreased the level of ET-1 and ACE and significantly increased the level of NO and activity of eNOS and SOD. At the same time, cilazapril extenuated hypoxia-induced injuries of EC. CONCLUSION:The results indicate that damaging structure and dysfunction of EC existes in hypoxic rats. The cilazapril effectively preventes and treates the chronic hypoxic PH by relieving the injury and improving secretion in EC.  相似文献   
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