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101.
鸡GDF-5基因外显子1多态性与鸡骨骼发育性状的相关研究 总被引:1,自引:0,他引:1
根据GenBank发表的鸡GDF-5基因的mRNA序列设计引物,以白耳鸡和东北农业大学选育的肉鸡高、低腹脂系第8世代鸡群为试验材料,通过测序和PCR-SSCP的方法进行SNP检测和基因型分析,探讨GDF5基因多态性与鸡生长和骨骼发育性状之间的关系。结果发现在GDF-5基因外显子1的84bp处存在1个C/T的突变位点,对该突变位点在研究群体中进行基因型分析,结果产生3种基因型,AA型个体的基因序列和GenBank(Accession No:AF123389)中的一致为C,而BB型个体的基因序列在84bp处突变为T。基因型与鸡体组成性状的统计分析结果表明,AA基因型个体的跖骨围显著高于AB基因型个体(P〈0.05);AA基因型和AB基因型个体的跖爪重和跖爪率显著高于BB基因型个体(P〈0.05);AB基因型个体的股骨长和股骨重显著高于BB基因型个体(P<0.05);表明该基因对鸡的骨骼性状有较大的影响或与控制骨骼发育性状的主效基因相连锁。 相似文献
102.
ZHOU Dun-hua WEI Jing HUANG Shao-liang FANG Jian-pei BAO Rong WU Yan-feng LI Yang 《园艺学报》2006,22(8):1606-1609
AIM:To study the isolation,expansion and purification of mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB),and investigate some biological identities of MSCs.METHODS:(1) MSCs of UCB,adult bone marrow (BM) and fetus BM were isolated by centrifugation with Ficoll,and the different kinds of MSCs were observed everyday.(2) Surface markers of MSCs were identified by flow cytometry.(3) The level of HGFs (TPO,SCF,FLT-3L,IL-6) secreted by different sources of MSCs was checked by ELISA method.RESULTS:(1) No difference in morphology of the colonies between UCB MSCs and BM MSCs was observed.However,the mononuclear cells needed in culture of UCB MSCs was about 3 times more than that in culture of BM MSCs.The times of UCB MSCs colony formation and confluencing were longer than that of BM in primary culture.(2) After passaged,there was no significant difference in the proliferation rates of 3 kinds of MSCs.Only 4 of 15 UCB samples contained a homogeneous population of MSCs.(3) UCB MSCs shared the same markers with BM MSCs.Neither hematopoietic marker nor immunologic recognition antigens were expressed.(4) The level of hematopoietic growth factors (HGFs) secreted by 3 kinds of MSCs was similar.CONCLUSIONS:(1) MSCs were isolated from UCB,but the amount of MSCs in UCB was smaller than that in BM,and just seldom samples of UCB contained homogeneous MSCs.(2) MSCs from UCB and BM shared the same biological characteristics,such as proliferation ability,surface markers,immunophenotypes and HGFs secretion. 相似文献
103.
“中国园艺学会热带南亚热带果树分会”是由广东省农业科学院果树研究所、福建省农业科学院果树研究所、仲恺农业技术学院园艺系、中国热带农业科学院南亚热带作物研究所等单位联合申请,并经中国园艺学会第九届六次常务理事会扩大会议讨论同意成立的。经筹委会讨论决定,将于200 相似文献
104.
WENG Wen-jun FANG Jian-pei WU Yan-feng XIAO Lu-lu XU Hong-gui BAO Rong WEI Jing XU Lü-hong 《园艺学报》2007,23(11):2200-2204
AIM: To probe the effect of different panel reactive antibody(PRA) serum levels from patients with β-thalassemia on the proliferation and differentiation potential of the hematopoietic stem /progenitor cell of cord blood.METHODS: 1×105 mononuclear cells (MNCs) isolated from umbilical cord blood were incubated with different PRA serum levels (0 μL,50 μL,100 μL) respectively and complement,inoculated into the methylcellulose cultural system.The proliferation and differentiation potential of the hematopoietic stem /progenitor cell of cord blood by the colony formation assay were detected on day 7 and day 14,respectively.RESULTS: After culture of 7 days,the total colonies and CFU-GM were 88.20±9.41,79.00±11.39 in group A and 88.60±9.12,79.20±10.44 in group B,which were significantly higher than those of 20.60±7.39,15.20±4.66 in group C and those of 4.00±2.05,1.40±0.51 in group D (P<0.01).Meanwhile compared with group A and group E,no significant difference was found (P>0.05).After culture for 14 days,the total colonies and CFU-GM were 216.00±31.10,117.40±24.80 in group A and 213.20±31.06,116.00±19.75 in group B,which were significantly higher than those of 97.80±14.43,32.80±8.10 in group C and those of 31.40±13.41,8.40±4.30 in group D (P<0.01).The CFU-GEMMs were 45.60±8.51 in group A and 42.60±7.03 in group B,which were significantly higher than those of 20.80±6.96 in group C and those of 7.80±6.06 in group D (P<0.05).The BFU-MK was 12.80±4.42 in group A and 11.00±2.74 in group B respectively,which were significantly higher than that of 1.00±0.55 in group D (P<0.05).The CFU-E in group B 17.20±4.03 was significantly higher than that of 5.60±2.87 in group D (P<0.05).Meanwhile compared with group A and group E,no significant difference was found (P>0.05).By the Kendall test,there were negative correlations between the level of PRA serum and the total colonies,CFU-GM on day 7,the total colonies,CFU-GM,CFU-GEMM,BFU-E,BFU-MK on day 14 (tau-b=-0.793,-0.849,-0.808,-0.804,-0.645,-0.674,-0.624,P<0.01).There was a negative correlation between the level of PRA serum and CFU-MK on day 14 (tau-b=-0.466,P<0.05).CONCLUSION: PRA sera inhibit the colony in the colony cultures of the hematopoietic stem cells/progenitor cells in cord blood.The inhibition depends on the level of PRA sera.The higher the level of PRA sera,the stronger the inhibition is observed in our study. 相似文献
105.
用包衣量为1:15~1:75的3%辛硫磷微胶囊种衣剂进行玉米室内发芽试验,结果证明其对当地主栽玉米品种的发芽、成苗安全;通过对不同叶龄玉米植株叶面喷雾的室内、外试验,表明该药剂剂型对玉米的叶面接触安全性高于同剂量的乳油。 相似文献
106.
107.
青海省引种俄罗斯沙棘的可行性分析 总被引:3,自引:0,他引:3
根据我国经济林发展高产优质的目标,应用生境因子分析法的理论,分析了青海海东地区引种俄罗斯沙棘的可行性,指出了引种中可能出现的问题和解决办法,提出在驯化的基础上,开展中国沙棘优良类型和俄罗斯沙棘的杂交种,才能选育出适合青海省区域化栽培的优良品种。 相似文献
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110.
鸡肉和鸡蛋中金刚烷胺与金刚乙胺残留检测UPLC-MS/MS法研究 总被引:1,自引:1,他引:0
建立了鸡肉和鸡蛋中金刚烷胺与金刚乙胺残留检测的超高效液相色谱-串联质谱方法(UPLC-MS/MS)。样品在酸性条件下乙腈提取后,用正己烷去除脂肪,高速离心去除蛋白质等杂质,上机测试。液相色谱条件:色谱柱为BEHC18(50×2.1mm,1.7μm),流动相为0.1%甲酸乙腈溶液+0.1%甲酸水溶液,流速0.3mL/min,柱温30cI二,进样量10μL。质谱条件:电喷雾离子源(ESI^+),多反应监测(MRM)方式进行采集。结果表明:金刚烷胺与金刚乙胺在2~100ng/mL浓度范围内均呈现良好的线性关系,相关系数(R。)分别为=0.9977和0.9988;鸡蛋和鸡肉中金刚烷胺与金刚乙胺残留检测方法检测限均为1ng/g,定量限均为2ng/g。从鸡肉组织在2、5和10ng/g三个添加浓度检测结果可以看出,金刚烷胺与金刚乙胺的平均回收率分别为79.6%~107.5%和78.4%~101.2%,批内和批间RSD均小于15%。从鸡蛋在2、5和10ng/g三个添加浓度检测结果可以看出,金刚烷胺与金刚乙胺的平均回收率分别为90.8%~111.1%和75.4%~87.4%,批内和批间RSD均小于15%。该方法具有简便快捷、灵敏度高、定性准确等特点。 相似文献