Einfluss selektiver mechanischer Fruchtbehangsregulierung auf Ethylensynthese als Stressindikator sowie Ertrag und Fruchtqualität bei Kernobst

The overall objective of this work was to improve fruit quality, break alternate bearing and reduce hand thinning using fewer chemicals in fruit crops. A device was constructed for mechanical thinning, which consisted of three independent horizontal rotors with ropes and freely adjustable angles on a frame, mounted on a front three point hitch and powered by the tractor hydraulics. This can be adapted to any fruit tree trained as spindle, Solaxe, (tall) vertical axis or fruit wall (le mur fruitier) irrespective of rootstock employed. Rotor speed varied from 300 to 460?rpm at either 5 or 7.5?km/h tractor speed. Eight-year-old or twelve-old apple trees cvs. ‘Gala’ and ‘Golden Delicious’ were mechanically thinned in 2007 between pink bud and full bloom (flower bud stages 6–8 or F1–F2) near Bonn, Germany; non-thinned and hand-thinned apple trees of the same block and variety served as control. Mechanically thinned flowering branches showed a similar amount of ethylene efflux (0.4–0.6?ppm C₂H₄/branch) as non-thinned flower branches, preventing potentially unexpected subsequent fruit drop, except for those removed by the rotors. The impact of the horizontal rotors on the branches was from the upper side and removed excessive flowers right to the tree trunk viz. the centre of the tree canopy, where fruits of lesser quality are expected leaving 2–3 flowers per cluster. Leaf damage was less than??10%, even at the fast rotor speed of 420?rpm, which was associated with negligible wood injury. Mechanical thinning induced firmer and sweeter fruit, i.e. tastier apples with longer shelf life, relative to control fruit from non-thinned apple trees. The greatest efficacy in terms of final fruit quality in the grading/sorting was achieved by a rotor speed of 360?rpm at a tractor speed of 5?km/h: Fruit mass increased by up to 20?g and the proportion of fruit larger than 70–75?mm by 10–30% compared with the fruit from non-thinned trees. Mechanical thinning with this newly constructed device led to a 10–20% reduction in yield, but increased returns due to better fruit size and colouration in apple with the potential to overcome alternate bearing.     

制动器耗散功率原理在ABS标定匹配中的应用

为解决ABS逻辑门限值方法匹配成本高、周期长的问题,将制动器耗散功率原理应用于匹配中.结合逻辑门限值与制动器耗散功率的各自优点,将制动器耗散功率原理能自适应识别各种路面状况的特点应用于匹配中;根据制动器耗散功率极大值对应的滑移率略小于临界滑移率且相差不大的原理,采用制动器耗散功率极大值对应的滑移率代替峰值附着系数对应的滑移率对ABS进行控制;滑移率与制动器耗散功率曲线获得较为简单,因此可以大大提高匹配效率.经过实车匹配验证,本方法提高了匹配效率和控制质量,节约了匹配成本.     

Application of the rpoS gene for the detection of Vibrio anguillarum in flounder and prawn by polymerase chain reaction

Vibrio anguillarum , an opportunistic fish pathogen, is the main species responsible for vibriosis, a disease that affects feral and farmed fish and shellfish, and causes considerable economic losses in marine aquaculture. In this study, we used polymerase chain reaction (PCR) to detect V. anguillarum . PCR specificity was evaluated by amplifying the rpoS gene, a general stress regulator, in six strains of V. anguillarum and 36 other bacterial species. PCR amplified a species-specific fragment (689 bp) from V. anguillarum . Furthermore, the PCR assay was sensitive enough to detect rpoS expression from 3 pg of genomic DNA , or from six colony-forming units (CFU) mL⁻¹ of cultured V. anguillarum . However, the assay was less sensitive when genomic DNA from the infected flounder and prawn was used (limit of detection, 50 ng and 10 ng g⁻¹ tissue, respectively). These data demonstrate that PCR amplification of the rpoS gene is a sensitive and species-specific method to detect V. anguillarum in practical situations.     

比格犬胫神经皮层体感诱发电位正常值研究

为了测定比格犬胫神经皮层体感诱发电位(somatosensory evoked potential,SEP)的波形及其潜伏期和波幅的正常值。选用15只健康比格犬,在异氟烷吸入麻醉状态下,使用肌电诱发电位仪电刺激其胫神经,将自制参考电极和记录电极分别放置于头部Fz处和Cz处记录SEP波形。结果表明:15只比格犬刺激左右侧胫神经均可记录到清晰的双向SEP波。刺激左侧胫神经引发的波形潜伏期为P(21.71±1.978)ms、N(32.61±2.568)ms、P-N(10.90±1.338)ms,其变异系数分别为9.11%、7.87%、12.88%;波幅为(2.762±1.230)μV,变异系数为41.47%;刺激右侧胫神经引发的波形潜伏期为P(21.49±1.985)ms、N(32.55±2.359)ms、P-N(11.06±1.153)ms,其变异系数分别为9.24%、7.25%、10.24%;波幅为(2.634±1.092)μV,变异系数为44.55%。潜伏期和波幅左右侧差异均不显著(P0.05)。对正常比格犬胫神经皮层SEP的测定,获得了清晰的SEP波形及各波潜伏期和波幅的正常值,印证了潜伏期的稳定性优于波幅的稳定性,可以为实验室研究犬病理状态下的SEP和宠物临床应用SEP诊断疾病提供参考依据。     

5种动物源食品中的氟虫腈及其3个代谢物残留量检测

利用QuEChERS技术结合超高效液相色谱-串联质谱的方法检测5种动物源食品(鸡肉、鸡蛋、猪肝、牛奶、猪肉)中氟虫腈及其3个代谢物残留量。样品用乙腈提取,经低温处理,多壁碳纳米管(MWCNTs)、弗罗里硅土(Florisil)和C18分散固相萃取(d-SPE)净化,外标法定量。在不同浓度的加标水平下,氟虫腈及其代谢物在5种动物源食品中的平均回收率为72.1%～113.8%,相对标准偏差RSD为1.9%～17.6%,检出限LOD在0.73～1.94μg/kg范围内,定量限LOQ在5～10μg/kg范围内。该方法具有简单、灵敏、准确等优点,适用于动物源食品中氟虫腈及其代谢物的快速筛查和定量检测。     

丘陵地区光伏智能施肥灌溉系统设计

生态农业的发展离不开施肥灌溉等设施的支持。本文介绍了光伏智能施肥灌溉系统的相关配置方案、设计方法、设备选型及计算方式;设计了一套以独立光伏提供能源动力、以GRPS提供无线远程控制,并结合现有智能化施肥灌溉技术的智能施肥灌溉系统,为丘陵山区农作物远程智能化施肥灌溉提供了新的解决方案。     

水稻施用植物生长促进菌效果初探

通过田间试验,对一种植物生长促进菌(PGPR)在水稻上的应用效果作了初步探讨。试验结果表明,PGPR制剂与化肥配合施用对水稻生长发育产生了显著的影响。与单施氮、磷、钾化肥相比,PGPR与化肥处理显著提高了水稻的株高、穗数、穗粒数、千粒重,比单施化肥和不施肥对照分别增产26.0%和53.9%。同时,PGPR处理提高了水稻的根系活力,并对灌浆速率产生了积极的影响。     

基于ANSYS的间隙式永磁吸附爬壁机器人的磁场分析

针对永磁吸附爬壁机器人,提出了一种新型的采用了矩形永磁体的间隙式吸附方式,并在Ansys的Magnetic-nodal环境下进行了永磁体的磁场分析,得出不同间隙条件下的磁场变化规律和吸附力。永磁体的磁场强度采用数值计算得出解析表达式的方法通常比较复杂。而有限元分析的方法则为磁场分析提供了一条快速、有效的路径。     

不同毛沟布置方式下农田次降雨氮素流失特性分析

为了研究田间工程对农田氮素随径流流失的影响,该文通过设置不同排水毛沟布置方式下农田对比试验,观测研究了农田出口的降雨径流特征以及氮素流失特征,结果表明：与毛沟布置多的处理相比,毛沟布置少的处理径流对雨强变化敏感度相对较小,其径流峰值滞后,且高峰径流量持续时间长,但总氮输出负荷减少7.44%;2个处理之间铵态氮和硝态氮的流失量均差异显著;在径流初始及峰值阶段铵态氮占总氮的比例显著高于径流消减阶段,硝态氮的比例全过程变化不大;硝态氮、铵态氮、总氮的输出负荷与径流量呈线性正相关。研究表明：汇水面积小,毛沟布置多的处理减少农田涝渍效果明显,而汇水面积大,毛沟布置少的处理能有效降低总氮的输出负荷。     

漩涡泵内部不稳定流场数值模拟

为了研究漩涡泵内部流场对其外特性的影响,采用RNGk-ε湍流模型和结构化网格技术对漩涡泵内部非定常流动进行数值模拟计算。结果表明,漩涡泵流道内压力变化呈直线分布;流道内纵向漩涡随流量的增大而减小,径向漩涡周期性地脱离叶片被液流带走且随着时间增加而增大;设计工况下,各监测点处压力脉动变化呈周期性分布,从流道进口到出口压力脉动幅值逐步增大;不同工况下,叶片通过频率是压力脉动的主频,其对应的压力脉动幅值随流量增大而增大。