Comparison of a new gold-immunochromatographic assay for the detection of antibodies against avian influenza virus with hemagglutination inhibition and agar gel immunodiffusion assays

A gold-immunochromatographic test-strip kit is used for the detection of IgG antibodies against the nucleocapsid protein of Avian Influenza Virus (AIV). Compared with the "gold standard", i.e. hemagglutination inhibition (HI) and agar gel immunodiffusion (AGID) assays, the gold-immunochromatographic test strip has many advantages, such as high specificity, high sensitivity, convenience, is rapid and has low cost. The gold-immunochromatographic test strip provides a unique tool for the on-site surveillance and diagnosis of Avian Influenza.     

丰帅苹果品种选育报告

丰帅是江苏省丰县农委果树研究所从金帅中选育的芽变品种,于2011年通过江苏省果茶花品种鉴定,鉴定证号为苏鉴果201109号。该品种在保留金帅优良特性的基础上,具有果实高桩、果面光洁、果锈较少的特点,适合在黄河故道区域发展。     

不同菜豆品种4种抗营养因子水平差异分析

菜豆中植物凝集素、皂苷、胰蛋白酶抑制剂和植酸等抗营养因子是引起菜豆食物中毒的内源物。以56份食荚菜豆品种为材料,测定了鲜荚中4种抗营养因子含量或活性。结果表明,供试菜豆品种群体4种抗营养因子水平均存在极显著差异。植物凝集素含量(均值为1.743 mg·g~(-1))和胰蛋白酶抑制剂活性(均值为1.680 mg·g~(-1))变异系数均在100%以上,品种频率分布曲线主峰明显,但在极高和极低区域均有品种间断分布,出现了品种水平的数量等级差异;皂苷含量(均值为3.730 mg·g~(-1))和植酸含量(均值为3.102 mg·g~(-1))变异系数均低于41%,品种频率分布曲线呈近正态分布,主峰明显,双尾有低频率连续分布。相关分析表明,植物凝集素含量与胰蛋白酶抑制剂活性呈极显著正相关。聚类分析将供试56份菜豆品种划分为3个品种群,近80%品种聚于抗营养因子中等水平品种群,近12%品种居于较低水平。由于菜豆植物凝集素是食用致毒性的主要内源物,同时其和胰蛋白酶抑制剂等均与菜豆田间抗病虫性密切相关,预示着筛选出极低水平植物凝集素的菜豆品种是可行的,但同时可能会降低其田间抗病虫性。     

晚熟苹果新品种‘瑞阳’

‘瑞阳’是以‘秦冠’ב富士’选育出的晚熟苹果新品种。果形端正,果个较大,平均单果质量282.3 g;底色黄绿,全面着鲜红色,果面光洁,果点小;肉质细脆,多汁,味甜,具香气,品质优,可溶性固形物含量16.5%,可滴定酸0.33%,硬度7.21 kg·cm-2,耐贮藏。在陕西渭北地区10月中旬成熟。     

Effect of rhynchophylline on TGF-β1/Smad pathway for processing ventricular remodeling in spontaneously hypertensive rats

AIM: To investigate the effect of rhynchophylline (Rhy) on blood pressure, cardiac hypertrophy and myocardial fibrosis in spontaneously hypertensive rats (SHR). METHODS: Spontaneously hypertensive rats were randomly divided into model group, high dose (10 mg·kg^-1·d^-1) and low dose (2.5 mg·kg^-1·d^-1) group of rhynchophylline, captopril group (17.5 mg·kg^-1·d^-1). Wistar-Kyoto rats were used as normal control. Respectively, systolic blood pressure was measured by tail cuff every 2 weeks. After 10 weeks, heart weight index and left ventricular weight index were calculated. The myocardial hydroxyproline and plasma angiotensin Ⅱ were detected. Moreover, basic myocardial histopathological changes and myocardial collagen fibres were observed by HE staining and Masson staining, respectively. The protein expression of TGF-β₁ and Smad3 in the myocardium was measured by the methods of immunohistochemistry and Western blot. RESULTS: Compared with SHR model group, Rhy significantly reduced blood pressure (P<0.05), the levels of HYP in the myocardium (P<0.05) and the levels of AngⅡ in the plasma (P<0.01). The pathological damages of the myocardial tissues and collagen deposition were attenuated. The protein expression of TGF-β₁ and Smad3 was significantly reduced by the treatment with Rhy (P<0.01). CONCLUSION: Rhynchophylline reduces blood pressure and adjusts to improve ventricular remodeling of SHR. The mechanism may be involved in the TGF-β₁/Smad pathway and reducing AngⅡ content.     

不同葡萄品种枝条栽培料对杏鲍菇生长的影响

以"维多利亚"、"户太"、"乍娜"3个鲜食品种的葡萄枝条为主要栽培料,以杏鲍菇为研究对象,探索不同品种葡萄枝条对杏鲍菇菌丝生长情况及生物学转化率的影响。同时对杏鲍菇不同生长阶段进行营养成分分析,确定最佳采收期。结果表明:"维多利亚"葡萄枝条栽培杏鲍菇的营养成分较好,且菌丝生命力旺盛。杏鲍菇菌柄长度为4、10cm时采收最佳,营养成分含量高,口感性好。     

化学调节剂诱导转Bt基因棉花杀虫蛋白和主要抗虫次生物的变化

初步探讨了水杨酸和缩节胺处理转 Bt基因棉花后顶端嫩叶中 Bt杀虫蛋白和主要抗虫次生物发生的变化。结果发现 ,水杨酸和缩节胺处理不能明显影响转 Bt基因棉花顶端嫩叶中 Bt杀虫蛋白的表达量 ;适宜浓度的水杨酸和缩节胺处理能够明显提高转 Bt基因棉花顶端嫩叶中缩合单宁和芸香苷的含量 ;适宜浓度的水杨酸处理还能够明显提高转 Bt基因棉花顶端嫩叶中总抗虫萜烯类物质的含量 ;而缩节胺处理对转 Bt基因棉花顶端嫩叶中总抗虫萜烯类物质的含量影响不大。水杨酸和缩节胺能够在一定程度诱导棉花抗虫次生物的表达     

微氧处理对干红葡萄酒酚类物质的影响

研究了微氧处理对葡萄酒酚类物质的影响,分析了单宁、花色素苷、盐酸指数、明胶指数和 PVP指数的变化。结果表明:适宜的微氧处理可以促进游离态花色素苷向结合态转化,促进单宁聚合 等;相关分析表明单宁、盐酸指数、明胶指数间两两相关,游离花色素苷与其他指标呈负相关。     

亚种间杂交稻穗颈节间组织与物质运转关系的研究

以品种间杂交稻为对照，对亚种间杂交稻干物质运转与穗颈节间某些性状的关系进行了研究。结果表明：杂交水稻结实期间茎鞘干物质输出率与穗净增重、最终穗重及灌浆速率呈负相关；穗颈节间单位维管或单位韧皮面积上通过的干物质量与灌浆速率显著正相关；齐穗后10d灌浆速率与穗颈节间过氧化氢酶活性显著正相关。     

营养液条件下油酸对黄瓜幼苗生长的影响

水培条件下，研究不同油酸水平对黄瓜幼苗生长的直接影响。与对照相比，生物量和生理指标等表明，油酸10μL/L水平对黄瓜生长几乎没有负面影响，而油酸水平为100，500，1000μL/L则表现出一定的抑制效应。其中，1000μL/L水平中黄瓜的生长明显受到抑制。试验还表明，油酸对黄瓜幼苗没有表现出直接的生长刺激效应。