Cyclic urinary leukopoietic activity in gray collie dogs

The urinary activities for bone marrow colony formation were measured on consecutive 24-hour urine samples from two gray collie dogs with cyclic neutropenia and from two normal collies. The activity varied cyclically in the gray collies with a peak activity developing during the neutropenic phase, which antecedes the return of blood neutrophils. The activity fell to undetectable levels after the blood neutrophil counts returned to the normal range. The urine of normal dogs showed no activity. Since the dogs with cyclic neutropenia have been shown to have periodic hematopoiesis, these data suggest a regulatory hormonal role for the substance measured by this assay.     

Calibration of sulfate levels in the archean ocean

The size of the marine sulfate reservoir has grown through Earth's history, reflecting the accumulation of oxygen into the atmosphere. Sulfur isotope fractionation experiments on marine and freshwater sulfate reducers, together with the isotope record, imply that oceanic Archean sulfate concentrations were <200 microM, which is less than one-hundredth of present marine sulfate levels and one-fifth of what was previously thought. Such low sulfate concentrations were maintained by volcanic outgassing of SO2 gas, and severely suppressed sulfate reduction rates allowed for a carbon cycle dominated by methanogenesis.     

Rare gases in lunar samples: study of distribution and variafions by a microprobe technique

The rare gas distribution in lunar soil, breccias, and rocks was studied with a micro-helium-probe. Gases are concentrated in grain surfaces and originate from solar wind. Helium-4 concentrations of different mineral components vary by more than a factor of 10 apart from individual fluctuations for each type. Also grains with no detectable helium-4 exist. Titanium-rich components have the highest, calcium-rich minerals the lowest concentrations. The solar wind was redistributed by diffusion. Mean gas layer thicknesses are 10, 6, and 5 microm for helium, neon, and argon respectively. Lithic fragments in breccias contain no solar gases. Glass pitted surfaces of crystalline rocks contain about 10(-2) cubic centimeter of helium-4 per square centimeter. Etched dust grains clearly show spallogenic and radiogenic components. The apparent mean exposure age of dust is approximately 500 x 10(6) years, its potassium-argon age is approximately 3.5 x 10(9) yerars. Cavities of crystalline rocks contain helium-4, radiogenic argon, H(2), and N(2).     

Ethnic differences in alcohol sensitivity

Japanese, Taiwanese, and Koreans, after drinking amounts of alcohol that have no detectable effect on Caucasoids, respond with a marked facial flushing and mild to moderate symptoms of intoxication. Group differences are present at birth, and are probably related to variations in autonomic reactivity.     

Molecular analysis of plant migration and refugia in the Arctic

The arctic flora is thought to have originated during the late Tertiary, approximately 3 million years ago. Plant migration routes during colonization of the Arctic are currently unknown, and uncertainty remains over where arctic plants survived Pleistocene glaciations. A phylogenetic analysis of chloroplast DNA variation in the purple saxifrage (Saxifraga oppositifolia) indicates that this plant first occurred in the Arctic in western Beringia before it migrated east and west to achieve a circumpolar distribution. The geographical distribution of chloroplast DNA variation in the species supports the hypothesis that, during Pleistocene glaciations, some plant refugia were located in the Arctic as well as at more southern latitudes.     

Livelihood change,farming, and managing flood risk in the Lerma Valley,Mexico

In face of rising flood losses globally, the approach of “living with floods,” rather than relying on structural measures for flood control and prevention, is acquiring greater resonance in diverse socioeconomic contexts. In the Lerma Valley in the state of Mexico, rapid industrialization, population growth, and the declining value of agricultural products are driving livelihood and land use change, exposing increasing numbers of people to flooding. However, data collected in two case studies of farm communities affected by flooding in 2003 illustrate that the concept of flood as agricultural “hazard” has been relatively recently constructed through public intervention in river management and disaster compensation. While farming still represents subsistence value to rural households, increasingly rural communities are relying on non-farm income and alternative livelihood strategies. In this context, defining flooding in rural areas as a private hazard for which individuals are entitled to public protection may be counterproductive. A different approach, in which farmers’ long acceptance of periodic flooding is combined with valuing agricultural land for ecoservices, may enable a more sustainable future for the region’s population.

Glutamatergic and dopaminergic neurons mediate anxiogenic and anxiolytic effects of CRHR1

The corticotropin-releasing hormone receptor 1 (CRHR1) critically controls behavioral adaptation to stress and is causally linked to emotional disorders. Using neurochemical and genetic tools, we determined that CRHR1 is expressed in forebrain glutamatergic and γ-aminobutyric acid-containing (GABAergic) neurons as well as in midbrain dopaminergic neurons. Via specific CRHR1 deletions in glutamatergic, GABAergic, dopaminergic, and serotonergic cells, we found that the lack of CRHR1 in forebrain glutamatergic circuits reduces anxiety and impairs neurotransmission in the amygdala and hippocampus. Selective deletion of CRHR1 in midbrain dopaminergic neurons increases anxiety-like behavior and reduces dopamine release in the prefrontal cortex. These results define a bidirectional model for the role of CRHR1 in anxiety and suggest that an imbalance between CRHR1-controlled anxiogenic glutamatergic and anxiolytic dopaminergic systems might lead to emotional disorders.     

Tritrichomonas foetus infection in purebred cats in Germany: prevalence of clinical signs and the role of co-infection with other enteroparasites

The aim of this study was to determine the prevalence of Tritrichomonas foetus infection and associated clinical signs in purebred cats in Germany, to investigate the role of co-infection, and identify determinants of infection. Faecal specimens accompanied by epidemiological questionnaires were scored and collected from 230 purebred cats. Faeces were examined for trichomonads and other enteroparasites. The prevalence of T foetus was 15.7% among cats and 18.5% among catteries. An abnormal faecal score and history of diarrhoea were observed in 64% and 61% of T foetus-positive cats, respectively, and correlated significantly with infection. Co-infection, observed in 36% of T foetus-infected cats, was not associated with diarrhoea. Norwegian Forest cats were infected significantly more often than other breeds. No association was found with any environmental factors. This study demonstrated a high prevalence of symptomatic T foetus infections in purebred cats in Germany. Co-infection with other enteroparasites did not worsen clinical signs of trichomonosis.     

Mitteilungen aus der Industrie

Mitteilungen

Mitteilungen aus der Industrie     

Intergeneric relationships in the Australian Vitaceae: new evidence from cpDNA analysis

Taxa related to important agricultural species are likely to contain a considerable amount of potentially valuable genetic diversity. Nevertheless, before breeding programs or gene discovery projects can be initiated it is important to understand the phylogenetic relationships between the species involved. A component of a major gene discovery project in grapes at the Centre for Plant Conservation Genetics (Southern Cross University, Australia) is directed at the discovery of novel genes in native Vitaceae. As a result a study was conducted in order to assess the phylogenetic relationships between V. vinifera and the native members of the three major Australian genera: Cayratia, Cissus and Tetrastigma. CpDNA sequence analysis (from the trnL intron) adequately resolved intergeneric relationship between the majority of the species studied and provided some useful new information on the phylogenetic relationships within the Vitaceae. This preliminary project identified two species, C. hypoglauca and C. sterculiifolia, as being closely related to V. vinifera and worthy of further in-depth investigation.