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11.
This review focuses on smart nano-materials built of stimuli-responsive (SR) polymers and will discuss their numerous applications in the biomedical field. The authors will first provide an overview of different stimuli and their corresponding, responsive polymers. By introducing myriad functionalities, SR polymers present a wide range of possibilities in the design of stimuli-responsive devices, making use of virtually all types of polymer constructs, from self-assembled structures (micelles, vesicles) to surfaces (polymer brushes, films) as described in the second section of the review. In the last section of this review the authors report on some of the most promising applications of stimuli-responsive polymers in nanomedicine. In particular, we will discuss applications pertaining to diagnosis, where SR polymers are used to construct sensors capable of selective recognition and quantification of analytes and physical variables, as well as imaging devices. We will also highlight some examples of responsive systems used for therapeutic applications, including smart drug delivery systems (micelles, vesicles, dendrimers...) and surfaces for regenerative medicine. 相似文献
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Katarzyna Płoneczka-Janeczko Paweł Lis Karolina Bierowiec Krzysztof Rypuła Paweł Chorbiński 《Veterinary research communications》2014,38(4):337-346
Bap and icaA genes are commonly known to be involved in the biofilm formation. The prevalence of bap and icaA genes and biofilm formation was determined in conjunctival isolates of coagulase negative staphylococci (CNS) collected from cats. The study was conducted on 90 archival CNS isolates collected from feline conjunctiva obtained from clinically healthy cats and cats with ocular problems. Biofilm formation was examined using the microtiter plate (MTP) method. The prevalence of icaA and bap genes was determined using polymerase chain reaction (PCR). Genetic profiles of the bap-positive isolates were examined using the modified random amplified polymorphic DNA (RAPD) method. Of the 90 CNS isolates investigated, 58.9 % (53/90) were confirmed to form biofilms on a polystyrene plate after 24 h, and the intensity of the biofilm production varied strongly between positive strains. Among the biofilm-producing isolates, 24.5 % (13/53) carried the icaA gene and 3.8 % (2/53) carried the bap gene. Among the isolates that did not produce biofilms, the icaA gene and bap gene were detected in 8.1 % (3/37) and 2.7 % (1/37) of isolates, respectively. This is the first report demonstrating that CNS isolated from feline conjunctiva can potentially be a bap gene reservoir. Preliminary comparison of the genetic profiles of three bap-positive isolates collected from cats showed that each of the isolates has a different genetic background with a high similarity with the human strain of S. epidermidis. 相似文献
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Błażej Poźniak Marta Tikhomirov Karolina Motykiewicz-Pers Kamila Bobrek Marcin Świtała 《Journal of veterinary pharmacology and therapeutics》2020,43(1):67-78
Enrofloxacin is a concentration-dependent antimicrobial used in bacterial infections in poultry. During a few months of a turkey's life, pharmacokinetics of drugs undergoes substantial changes which may compromise their efficacy due to variability in internal exposure (measured by area under the concentration–time curve, AUC). The aim of this study was to describe the effects of age on the pharmacokinetics of a single intravenous (i.v.) and oral administration of enrofloxacin at a dose of 10 mg/kg to turkeys. It was found that during a 2.5-month-long period of growth from 1.4 to 14.6 kg, the AUC after i.v. administration increased almost threefold due to a significant decrease in the body clearance (from a mean of 0.76–0.28 L hr−1 kg−1). Over the same period, the mean elimination half-life was prolonged from 2.65 to 7.03 hr. Oral administration resulted in a similar trend in pharmacokinetic parameters. For both routes, formation of the major metabolite, ciprofloxacin, was marginal. Protein binding was not age-dependent and never exceeded 50%. Body clearance, volume of distribution and elimination half-life were subjected to an allometric analysis and a novel, nonlinear dosage protocol has been proposed to improve the internal exposure to the drug in different age groups of turkeys. 相似文献
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Andrzej?BobiecEmail author Ewelina?Jaszcz Karolina?Wojtunik 《European Journal of Forest Research》2011,130(5):785-797
The oak (Quercus robur L.) regeneration intensity was assessed in the core area of the Białowieża National Park (BNP) in Poland with respect to
the selected ecological factors. The emphasis was placed on the response of oak regeneration to disturbances, including the
large-scale dieback of spruce stands. Defining their effect could help predicting the role of oak in naturally developing
lowland forest ecosystems in the European hemiboreal zone. The results of the study challenge the opinion that the ‘lime-oak-hornbeam
forest’ is a ‘climax’ community, confirming a very poor regeneration represented by only two saplings taller than 0.5 m per
hectare. By contrast, in spruce-dominated communities, from 49 to 848 taller saplings per hectare were found. The occurrence
of saplings was associated with discontinuous canopy of late seral stage of stands, as well as with large gaps. Most of the
best quality grown-up oak saplings developed in the immediate neighbourhood of spruce logs. The results of the research indicate
that ‘lime-oak-hornbeam forest’ (Tilio-Carpinetum) should be rather perceived as a transient community, evolved from relict, culturally modified, oak woodlands. Because spruce
had become a dominating species only after abandoning in mid-1800s the historical regime of anthropogenic disturbances (involving
frequent forest fires), the observed phenomena related to the disintegration of spruce stands had probably no precedent over
the last 500 years. To confirm whether the massive decline of spruce stands will finally result in the successful establishment
of the new canopy oak generation, both in Białowieża and other forests of hemiboreal zone, further research is needed. 相似文献
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Szafranek B Chrapkowska K Waligóra D Palavinskas R Banach A Szafranek J 《Journal of agricultural and food chemistry》2006,54(20):7729-7734
The structures of two previously unknown sesquiterpene alcohols of the potato (Solanum tuberosum) were assigned. The potato alcohols were obtained by steam-distillation, preparative column chromatography, and separation into fractions by HPLC on a silica gel column. The fractions were studied by GC-FID, GC-MS, and NMR spectroscopy. The potato sesquiterpene alcohols were identified as kunzeaol (6-alpha-hydroxygermacra-1(10),4-diene) and ledol. These two compounds were used in feeding tests with larvae and beetles of the Colorado potato beetle (Leptinotarsa decemlineata Say). In a bioassay, kunzeaol was found to act as a feeding attractant for the beetles. 相似文献
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In two calves with massive cysticercosis and one healthy control calf the method of electrocardiographic examination was applied to a preciser determination of the clinical finding. The examination was performed on the 75th day after the experimental infection. Singly recorded electrocardiographic findings provided no characteristic symptoms indicating an injury of the conductive system of the heart. However, in both infected calves a lowering of the pulse frequency was found and in one of them also a flattening and a change of the polarity of the T wave. Autopsy and histological examination of the heart of the infected calves performed 111 days and 250 days after the infection revealed an attacking of the cardiac muscle by cysticerci and dispersed chronic myocarditis. This finding and the investigated dynamics of pathological changes in the course of cysticercosis in other experimental animals indicate that at the time of the electrocardiographic examination cysticercoid myocarditis at a stage of rest was recorded. 相似文献
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