Protein kinase C regulates ezrin-radixin-moesin phosphorylation in canine osteosarcoma cells

The development of metastasis is the most significant cause of death for both canine and human patients with osteosarcoma (OS). Ezrin has been associated with tumour progression and metastasis in human, canine and murine OS. Ezrin activation is dynamically regulated by protein kinase C (PKC) during metastatic progression in human and murine OS. To include the dog in the development of therapeutics that target ezrin biology, we characterized four new canine OS cell lines and confirmed the relationship between PKC and ezrin in these cells. Three of four cell lines formed tumours in mice that were histologically consistent with OS. All cell lines were markedly aneuploid and expressed ezrin and PKC. Finally, both ezrin phosphorylation and cell migration were inhibited using a PKC inhibitor. These data suggest that an association between PKC-mediated activation of ezrin and the metastatic phenotype in canine OS cells.     

Early detection of bovine respiratory disease in young bulls using reticulo-rumen temperature boluses

The use of reticulo-rumen temperature boluses to detect bovine respiratory disease (BRD) was investigated in young bulls following their entry into a fattening unit. Twenty-four bulls received a bolus at entry and were observed for 40 days. As soon as a reticulo-rumen hyperthermia (RH) episode was detected using the bolus, clinical examination was performed by a veterinarian and then repeated every 12-24h until the end of RH episode. Fifty-two RH episodes were detected in 22 animals. High rectal temperatures (40.1±0.6°C) were observed during these episodes. BRD was diagnosed on the basis of clinical examination during 38/52 RH episodes in 21 animals (positive predictive value 73%). The onset of BRD signs always occurred after the onset of RH episodes, with a time-lag from 12 to 136 h, depending on BRD signs. Monitoring reticulo-rumen temperature permits early detection of BRD; however, clinical examination is required to confirm BRD.     

不同蛋白质水平对中卫山羊妊娠母羊日粮消化性能的影响

选择体重、年龄相近健康的妊娠母羊30只,随机分为3组。每组10只。采用相同能量水平、不同蛋白质水平的日粮饲养。试验结果表明,在本试验条件下,日粮蛋白质含量以116.57g/d比较有利于中卫山羊妊娠母羊的生长发育。     

绿壳蛋和乌鸡蛋胆固醇含量的测定

为了研究绿壳蛋和乌鸡蛋的胆固醇含量,试验对比色法和高效液相色谱法进行比较后,选用高效液相色谱法对卢氏绿壳蛋鸡,丝羽乌骨鸡两个品种的鸡蛋胆固醇含量进行测定.试验结果表明:比色法的测定结果显著高于高效液相色谱法的测定结果(P<0.05);绿壳蛋和乌鸡蛋的胆固醇浓度分别为7.75 mg/g和8.99 mg/g,绿壳蛋胆固醇浓...     

不同色板对Q型烟粉虱成虫的诱杀效果研究

在黄瓜大棚悬挂不同颜色的诱虫板,研究3种黄色和3种蓝色诱虫板不同朝向以及不同时段对烟粉虱成虫的诱杀效果。结果表明:与蓝色相比烟粉虱成虫对黄色具有较强的趋性,其中深黄色的诱杀效果尤为突出;悬挂诱虫板东、西、南、北4个方向中,南向诱虫数量最多;全天诱集烟粉虱成虫数量显示7:00~17:00诱集数量明显高于17:00~7:00。     

不同颜色诱捕器对梨小食心虫诱杀效果研究初报

利用5种不同颜色的悬挂式黏胶型诱捕器和梨小食心虫长效性诱剂诱芯在千亩桃园进行大面积防治梨小食心虫试验。结果表明:5种不同颜色的诱捕器对梨小食心虫的诱集效果之间差异不明显。蓝色诱捕器对防除梨小食心虫的其它昆虫的诱集量最大,明显多于另外4种颜色的诱捕器,但是另外4种颜色诱捕器诱集其它昆虫的数量之间差异不明显。     

生物反应堆技术在榆林市设施蔬菜上的应用效益分析

以生物反应堆技术在温室番茄和黄瓜栽培中的应用为实例,分析生物反应堆技术在榆林市设施蔬菜上的应用效益.结果表明:与对照相比,在这2种作物生产中,不仅提高了其品质指标,而且分别提高种植农户纯收益达46.33％和40.22％,且有利于改善农业生态环境.     

不同粒径砂砾石覆盖对砂田西瓜土壤微生物和酶活性的影响

为分析不同粒径砂砾石覆盖对砂田西瓜土壤微生物数量和酶活性的影响,2010年在位于黄土丘陵半干旱区的甘肃省庄浪县万泉镇境内进行了不同粒径砂砾石覆盖土壤特性研究.研究结果表明,与露地对照相比,砂砾石覆盖明显改善土壤中的根际微生物区系,显著提高各类微生物数量,增强酶活性;各粒径砂砾石覆盖下的土壤微生物数均为细菌＞放线菌＞真菌...     

阿里第四系地下水资源形成控制因素研究

西藏自治区阿里地区地处藏西北,位于世界屋脊之巅,地貌上以高原湖盆地带为主,属于极端缺水的贫困地区.干旱缺水严重制约了当地农牧业的发展,因此迫切需要查明该地区地下水资源的分布规律.文中从青藏高原隆升这个大的地质背景入手,详细分析阿里地质与水文地质条件,通过对阿里第四系地下水系统分析认为:本区受水汽运移、第四系松散堆积物成...     

吉林省薄皮甜瓜主产区白粉病菌生理小种的初步鉴定

2011年秋季对吉林省四个地区的不同乡镇,不同栽培模式的薄皮甜瓜上采集的共9个来源的白粉病菌进行生理小种鉴定。按照国际通用的瓜类白粉病生理小种鉴定标准体系,根据病菌在13个鉴别寄主上的抗感反应,初步确定吉林省瓜类白粉病菌存在单囊壳白粉菌（S.fuliginea）的两个生理小种,即小种1和小种2France,且主流小种为...