栽培密度、施肥量及有效含量对大豆产量的影响

本研究以牡丰7号为试验材料,研究栽培密度、施肥量及有效含量3方面因素对产量的影响,每个因素设置5个水平,采用三元二次正交旋转组合设计方法。结果表明,对产量影响因子效应的大小依次为有效含量>栽培密度>施肥量,适当增加施肥量、减小栽培密度将获得牡丰7号大豆的高产。     

籼粳亚种间核DNA特征性分子标记在美洲爪哇稻中的分布规律初步研究

对籼粳亚种间特征性SSR分子标记和ITS序列中特征性碱基在美洲爪哇稻中的分布特点进行了初步研究。结果表明,籼稻和粳稻特征性SSR分子标记在供试爪哇稻材料中都有分布,但不同爪哇稻品种所含籼稻或粳稻特征性分子标记的多少存在差异;通过对比ITS序列籼、粳特征性碱基,Lemont与籼稻一致,P002在4个位点与籼稻相同,2个位点与粳稻一致,另4个爪哇稻的ITS序列与粳稻完全一致。表明爪哇稻是介于籼、粳稻之间的中间类型,但不同爪哇稻品种偏籼或偏粳程度存在差异。     

犊牛传染性肺炎病原分析及其防治

犊牛肺炎的发病率高居不下,与平时的饲养管理方式关系密切。其病原复杂多样,更是增加了预防和治疗困难．了解犊牛肺炎的病原及地方流行性、改善提高饲养管理模式能够有效降低犊牛传染性肺炎的发病率和死亡率．     

山西紫草属植物一新记录种

紫草属植物山西记录有2种.2012年4月中旬,笔者在山西蟒河自然保护区进行考察时,采集到紫草科（Boraginaceae）紫草属（Lithospermum L.）野生植物梓木草（L.zollingeriD C.）,为山西紫草科植物增加了种级水平的新记录,迄今山西省己发现3种紫草属植物.     

密码子优化的鸡白细胞介素-17在昆虫细胞中的表达研究

为了获得具有良好生物学活性且具有较高表达量的鸡白细胞介素-17(ChIL-17),本研究在前期研究工作基础上,将ChIL-17的编码基因按照真核细胞(昆虫细胞)偏爱的密码子进行优化改造,经全基因合成后插入到转座载体pFastBacTM Ⅰ中,构建重组转移质粒pfast-mod.ChIL-17并转化DH10Bac感受态细胞.通过位点特异性转座将mod.ChIL-17基因整合到穿梭质粒Bacmid中,获得重组穿梭质粒Bacmid-mod.ChIL-17.应用脂质体将重组穿梭质粒转染Sf9昆虫细胞,获得重组杆状病毒rBac-mod.ChIL-17.重组病毒传代扩增感染Sf9细胞,通过间接免疫荧光检测目的蛋白的表达.结果表明,经过优化的ChIL-17在杆状病毒系统中获得表达.     

Survey of mycotic mastitis in dairy cows from Heilongjiang Province, China

A survey of the prevalence rate, pathogenic subspecies, and risk factors of mycotic mastitis in dairy cows from Heilongjiang Province, China, was conducted. Milk samples from 412 cows with chronic mastitis were collected and cultured on 8 % sheep blood agar, MacConkey agar, and Sabouraud agar with chloramphenicol. Counting of the morphologically distinct colonies was performed, as well as the isolation and identification of organisms through phenotypical and physiological criteria. Four hundred seventy-eight aerobic microorganisms were isolated. Yeasts and yeast-like fungi 35.6 % (170/478) and bacteria 64.4 % (308/478) were isolated. The fungal isolates were identified as Candida (79.4 %), Trichosporon (5.9 %), Aspergillus (7.1 %), Cryptococcus (2.4 %), and Rhodotorula (4.1 %). More than ten species of yeast were isolated including Candida krusei 50/135 (37 %), Candida rugosa 16/135 (11.9 %), and Candida lusitaniae 15/135 (11.1 %). A higher positivity (18.5 and 56.3 %) (P ≤0.05) was observed in cows from environmental temperatures of 0–15 and 15–35 °C than those at <0 °C and in cows affected by the disease for >45 and 30–45 days compared with cows suffering 10–30 days. Meanwhile, a statistically significant difference (44.9 vs. 31.4 %) (P ≤0.05) was observed under extensive raising systems vs. intensive raising systems. It appears that Candida is a major pathogen of mycotic mastitis of dairy cows. Extensive raising system, high environmental temperature (15–35 °C), and the duration of the disease (>30 days) were important risk factors of the incidence of mycotic mastitis. Here, we provide a theoretical foundation for research into preventing and treating mycotic mastitis of dairy cows in China.     

轻轨(地下段)振动环境影响分析及应用实例

指出了振动环境影响评价是城市轨道交通建设项目环境影响评价的重点评价专题,剖析了轻轨(地下段)振动环境影响评价重点值得关注的几个问题,结合实例进一步探讨了城市快速轨道交通对环境振动产生的影响,为污染防治提供依据。     

The Effect of Low-Dose Marine n-3 Fatty Acids on Plasma Levels of sCD36 in Overweight Subjects: A Randomized,Double-Blind,Placebo-Controlled Trial

CD36 is a scavenger receptor involved in lipid uptake and inflammation. Recently, non-cell-bound CD36 (sCD36) was identified in plasma and suggested to be a marker of lipid accumulation in the vessel wall. Marine n-3 polyunsaturated fatty acids (PUFA) may have cardioprotective effects. This study evaluated the effect of marine n-3 PUFA on sCD36 levels in overweight subjects. Fifty overweight subjects were randomized to 1.1 g of n-3 PUFA or 2 g of olive oil daily for six weeks. Neutrophils were isolated at baseline and after six weeks of treatment while an adipose tissue biopsy was obtained at baseline. The content of n-3 PUFA in adipose tissue and neutrophils was analyzed by gas chromatography, while plasma levels of sCD36 were determined using an enzyme-linked immunosorbent assay (ELISA). After six weeks of supplement plasma sCD36 did not differ between supplements (P = 0.18). There was no significant correlation between plasma sCD36 levels and n-3 PUFA in neutrophils at baseline (r = −0.02, P = 0.88), after six weeks supplement (r = −0.03, P = 0.85) or in adipose tissue (r = 0.14, P = 0.34). This study therefore does not provide evidence for a cardioprotective effect of n-3 PUFA acting through a CD36-dependent mechanism.     

Replication characteristics of swine influenza viruses in precision-cut lung slices reflect the virulence properties of the viruses

Precision-cut lung slices of pigs were infected with five swine influenza A viruses of different subtypes (A/sw/Potsdam/15/1981 H1N1, A/sw/Bad Griesbach/IDT5604/2006 H1N1, A/sw/Bakum/1832/2000 H1N2, A/sw/Damme/IDT5673/2006 H3N2, A/sw/Herford/IDT5932/2007 H3N2). The viruses were able to infect ciliated and mucus-producing cells. The infection of well-differentiated respiratory epithelial cells by swine influenza A viruses was analyzed with respect to the kinetics of virus release into the supernatant. The highest titres were determined for H3N2/2006 and H3N2/2007 viruses. H1N1/1981 and H1N2/2000 viruses replicated somewhat slower than the H3N2 viruses whereas a H1N1 strain from 2006 multiplied at significantly lower titres than the other strains. Regarding their ability to induce a ciliostatic effect, the two H3N2 strains were found to be most virulent. H1N1/1981 and H1N2/2000 were somewhat less virulent with respect to their effect on ciliary activity. The lowest ciliostatic effect was observed with H1N1/2006. In order to investigate whether this finding is associated with a corresponding virulence in the host, pigs were infected experimentally with H3N2/2006, H1N2/2000, H1N1/1981 and H1N1/2006 viruses. The H1N1/2006 virus was significantly less virulent than the other viruses in pigs which was in agreement with the results obtained by the in vitro-studies. These findings offer the possibility to develop an ex vivo-system that is able to assess virulence of swine influenza A viruses.     

黄秋葵品种比较试验

引进5个黄秋葵品种进行品种比较试验,结果表明:从台湾引进的五福黄秋葵品种表现早熟、耐热、抗病、优质、高产、株高适中、抗台风,适合福州平原地区种植。