全文获取类型
收费全文 | 46554篇 |
免费 | 1916篇 |
国内免费 | 3212篇 |
专业分类
林业 | 6480篇 |
农学 | 4453篇 |
基础科学 | 2099篇 |
6549篇 | |
综合类 | 12866篇 |
农作物 | 4144篇 |
水产渔业 | 3408篇 |
畜牧兽医 | 5356篇 |
园艺 | 2860篇 |
植物保护 | 3467篇 |
出版年
2024年 | 168篇 |
2023年 | 490篇 |
2022年 | 1137篇 |
2021年 | 1254篇 |
2020年 | 1236篇 |
2019年 | 1181篇 |
2018年 | 3533篇 |
2017年 | 3972篇 |
2016年 | 2237篇 |
2015年 | 1575篇 |
2014年 | 1543篇 |
2013年 | 1849篇 |
2012年 | 3163篇 |
2011年 | 4625篇 |
2010年 | 4376篇 |
2009年 | 3347篇 |
2008年 | 3324篇 |
2007年 | 3475篇 |
2006年 | 1736篇 |
2005年 | 1445篇 |
2004年 | 950篇 |
2003年 | 791篇 |
2002年 | 841篇 |
2001年 | 744篇 |
2000年 | 632篇 |
1999年 | 397篇 |
1998年 | 283篇 |
1997年 | 236篇 |
1996年 | 158篇 |
1995年 | 186篇 |
1994年 | 167篇 |
1993年 | 134篇 |
1992年 | 107篇 |
1991年 | 98篇 |
1990年 | 73篇 |
1989年 | 61篇 |
1988年 | 62篇 |
1987年 | 30篇 |
1986年 | 11篇 |
1985年 | 8篇 |
1984年 | 9篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1981年 | 7篇 |
1980年 | 3篇 |
1979年 | 4篇 |
1977年 | 4篇 |
1968年 | 4篇 |
1962年 | 2篇 |
1956年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
61.
三峡库区资源丰富,但库区农村存在资源利用不合理、能源紧缺的情况.库区的气候及资源是发展沼气的有利条件.沼气是一项投资少,见效快,一次投资长期受益的好项目;是能够解决库区农村燃料短缺,提高用能品质,促进农民增收节支的有效途径,并且是保护生态改善农村生活环境,促进库区农村生态经济可持续发展的有效举措. 相似文献
62.
本试验对送检的金丝雀经实验室诊断,确认为金丝雀痘,对分离的痘病毒进行动物致病性试验、病毒分离传代和致试验、致弱毒保护力试验和返强试验。结果表明:金丝雀痘病毒经鸡胚盲传3后能适鸡胚并致死金丝雀,8代后无致病性,13例毒性快速通过本动物3次无返强现象,金丝雀用第13代毒刺种30天能抵抗强毒攻击。 相似文献
63.
家蚕第2隐性赤蚁的遗传学研究——Ⅱ.ch-2基因的连锁分析 总被引:1,自引:1,他引:0
Ch-2基因是继ch之后新发现的一种隐性赤蚁ch-2基因与pM(2)、Ze(3)、L(4)、Pe(5)、E~(EL)(6)、q(7)、st(8)、I(9)、w-2(10)、ms(12)、cf(13)、U(14)、Se(15)、cts(16)、B_m(17)、nb(19)、rb(21)、or(22)、sp(23)、Nd(25)、及Y_m等各标志基因都是独立遗传.Ch-2与mln杂交F_2代分离+ch-2+min:ch-2+min:ch-2mln:ch-2mln=523:284:231:0≈2:1:1:0;ch-2与elp杂交F_2代分离+ch-2+elf:ch-2+elp:+ch-2elp:ch-2elp=219:97:68:0≈2:1:1:0,充分说明ch-2与mln、elp是连锁遗传的,即ch-2基因位于第18连锁群. 相似文献
64.
A. Schots J. De Boer A. Schouten J. Roosien J. F. Zil Verentant H. Pomp L. Bouwman-Smits H. Overmars F. J. Gommers B. Visser W. J. Stiekema J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location. 相似文献
65.
AIM: To investigate the contribution of angiotensin-converting enzyme inhibitor (ACEI) to the regulation of calpain system in infarcted myocardium. METHODS: Rat myocardial infarction (MI) model was established by permanent ligation of the left coronary artery. The treatment with the ACEI inhibitor rampril (1 mg·kg-1·d-1) was started 7 days prior to surgery. On day 1, 3, 7 and 14 after MI, protein levels of calpainⅠ, Ⅱ and calpastatin were determined in left ventricular free wall (LVFW), interventricular septum (IS) and right ventricule. RESULTS: CalpainⅠprotein level was increased in IS 14 d post MI, whereas the protein level of calpainⅡ was maximally increased in LVFW 3 d post MI. Rampril decreased protein up-regulation of calpainⅠ and Ⅱ, and reduced infarct size and interstitial fibrosis. Calpastatin protein expression was not affected by ACEI. CONCLUSIONS: CalpainⅠ is involved in cardiac remodelling in the late and calpainⅡ contributes to cardiac tissue damage in the early phase of MI. The heart protective effect of ACEI may be related to the inhibition of calpain system in the pathogenesis of myocardial infarction. 相似文献
66.
67.
68.
69.
不同贮藏温度对大五星枇杷果实保鲜效果的研究 总被引:4,自引:0,他引:4
枇杷成熟于初夏高温多湿季节,果实生理活动旺盛,含水量多,极易腐烂变质,一般条件下难以贮存和长途运销,采后损失率高。目前,枇杷贮藏最常用的方法是冷藏法。郑永华等把枇杷果实在1℃低温贮藏,可显著抑制果实失水,贮藏期可达20天以上;Mukerjece认为,枇杷贮藏在3℃低温下能减少腐烂;周德荣通过0、5、15℃和常温比较表明,“照种”枇杷在5℃下贮藏31天的好果率最高为83.9%。但近年来,一些研究报道,枇杷果实在不适宜的低温下长期贮藏会发生冷害现象。曾雅鹃等发现,在1℃低温下贮藏,枇杷果皮难剥,果肉变硬, 相似文献
70.
野生朱红硫磺菌驯化栽培研究 总被引:2,自引:2,他引:2
营养试验、菌丝体培养和出菇温度试验、培养基酸碱度试验、出菇条件试验结果表明:朱红硫磺菌菌丝体适宜生长的温度范围为15-32℃,最适温度范围为22~28℃;子实体发生的最适温度为16~25℃,气温低于16℃,子实体色泽为淡橙色,16℃以上,子实体呈鲜橙红色;培养料适宜营养C/N为20-26/1;朱红硫磺菌菌丝体适宜酸碱度范围为pH3.0~8.0,最适酸碱度为pH4.0—6.5;朱红硫磺菌的产量与光照条件无关,但光照强度影响子实体的发生时间和色泽;朱红硫磺菌栽培原料广泛,凡栽培木腐菌的培养料均可用于栽培朱红硫磺菌。 相似文献