马链球菌兽疫亚种类M蛋白亚单位疫苗小鼠免疫试验

为获得控制猪链球菌病安全高效的疫苗，本试验进行了马链球菌兽疫亚种类M蛋白亚单位疫苗的研制。应用热酸法提取马链球菌兽疫亚种ATCC35246株类M蛋白，并分别采用羟基磷灰石（HAT）层析和冷酒精沉淀法进行纯化，结果得到较纯的类M蛋白。用热酸提取的粗制类M蛋白、2种纯化的类M蛋白及全菌苗免疫小鼠，结果保护率分别为92％（11／12）、100％（12／12）、100％（12／12）和83％（10／12），表明类M蛋白亚单位疫苗有进一步开发的必要。     

LRH-3、PMSG、HCG对人工授精母蓝狐产仔效果影响的比较(Ⅲ)

通过PMSG +LRH - 3和PMSG +HCG的组合使用 ,结果表明 ,使用PMSG +LRH - 3的试验组母狐的产仔率比对照组提高了 2 0 % ,胎平均产仔数提高了 0 2只。而使用PMSG +HCG组合母狐的产仔率与对照组相同 ,但胎平均产仔数提高了 0 5只。     

胸膜莫肺炎放线杆菌APXIV部分基因片段的表达及纯化

参照GenBank中已发表的ApxIV基因序列,以自行分离的App DNA为模板,利用PCR方法扩增出ApxIV3'端,大小为552bp的保守基因序列.将PCR产物克隆到pMD18-T Simple Vector中,获得重组质粒pMD-ApxIV,对其重组质粒pMD-ApxIV进行BamHI、HindIII双酶切,并将酶切产物克隆到原核表达载体pET-32a(+)中,构建了重组表达质粒pET-ApxIV.将表达质粒转化至大肠杆菌BL21中,用IPTG诱导表达,通过SDS-PAGE和Western blot分析,结果表明pET-ApxIV在BL21中成功表达,并能被App阳性血清所识别,具有良好的免疫原性.表达蛋白的分子质量约为39.5KDa.利用HiTrap FF crude columns将表达的蛋白进行了纯化.     

Inductive expression of the NOD1 signalling pathway in chickens infected with Salmonella pullorum

1. The aim of this study was to describe the role of Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) receptor signalling in chicken.

2. Tissue-specific expression analysis of NOD1, receptor-interacting serine-threonine kinase 2 (RIPK2), nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase 11 (MAPK11 or p38) by quantitative real-time PCR (qRT-PCR) revealed their wide distribution in various organs and tissues.

3. Salmonella pullorum infection activated NOD1 receptor signalling in vivo and in vitro, resulting in significant induction of downstream signalling molecules RIPK2, NF-κB/p65, MAPK11/p38 and the effector molecules IL-1b and IL-8.

4. Activation of NOD1 by its agonist bacterial γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP) in HD11 cells induced the adapter molecular RIPK2 and activated the NF-κB/p65 and MAPK11/p38 pathways, resulting in an increase in IL-8 but not IL-1β. Additionally, inhibition of NOD1 using NOD1-shRNA resulted in downregulation of RIPK2, MAPK11 and IL-8, while NF-κB/p65 and IL-1β were unaltered.

5. These results highlight the important role of NOD1 receptors in eliciting the innate immune response following pathogenic invasion in chicken.     

江西养猪业的发展现状、存在问题及对策

<正>自2006年下半年以来,全国养猪生产逐步摆脱市场持续低糜的阴影,生猪及其产品价格不断上涨,养猪业呈现产销两旺的大好形势。江西是全国生猪主产省之一,每年外销和出口生猪1000万头左右,对保障全国肉类供应发挥了重要作用。2006年,江西省养猪业虽然受到市场大幅波动和动物疫病等不利因素影响,但总体上仍然保持稳定发展的势头。     

河南警犬参与侦破命案4例

2007年1月26日,河南省郸城县东风乡发生一起入室抢劫杀人案,受害人侯某被人杀死在自家床上,家中被抢走现金9700元。接指令后,淮阳县公安局带犬民警徐奎携警犬“追命”赶赴现场。在带犬民警的指挥引导下,     

大豆市场中长期走势向好

CBOT大豆价格自10月初开始上涨以来。涨幅已达到130美分／蒲式耳．本轮上涨行情跟大豆当前利空的供需基本面并不相符。行情的上涨最初是由小麦带动，随后则是由玉米带动，由于玉米长期前景看好，且美国玉米和大豆又处在同一生长带，玉米价格的上涨将促使农民多种玉米少种大豆．这样的前景终将导致大豆的供需平衡趋紧。因此，为了保证大豆面积不会大幅下降，大豆价格就只有跟随玉米上涨．这就造成了大豆出现本轮上涨行情。鉴于玉米价格长期看好的前景不会改变．我们认为大豆价格也进入了长期牛市行情之中．但是短期内不排除期价步入回调。下面从几个方面来论证我们的观点。     

浅谈鸡球虫病的免疫

鸡球虫病是一种危害性较大的肠道寄生虫病,多发于3个月龄以内的仔鸡.尤以15～45日龄的雏鸡最易感染,近年来,球虫疫苗应用,使工厂生产的养鸡场在鸡球虫病控制方面取得显著成绩.笔者就鸡球虫病免疫在生产实践中的应用,作肤浅论述.     

Identification of polymorphisms and association analysis with meat quality traits in the porcine KIAA1717 and HUMMLC2B genes

Skeletal muscle genes are potential candidates for production and meat quality. Screening a subtracted cDNA library constructed with mRNA obtained from longissimus dorsi muscles of F1 hybrids Landrace × Yorkshire and their female parents Yorkshire, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. Database search revealed KIAA1717 and HUMMLC2B encoded proteins with SET domain and EF-hand calcium binding motif, respectively. In the present work we identified their partial polymorphisms and two SNPs, one (C1354T) at the 3′ untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, both created/disrupted a restriction site for endonuclease Msp I. The selected pigs were genotyped at the KIAA1717 C1354T and HUMMLC2B A345G sites by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with meat marbling (longissimus doris (p < 0.05), biceps femoris (p < 0.01)) and intramuscular fat (p < 0.01). HUMMLC2B A345G were significantly associated with meat pH (longissimus doris (p < 0.05), biceps femoris (p < 0.01)), drip loss (p < 0.01), water holding capacity (p < 0.01) and meat color value (longissimus doris (p < 0.01), biceps femoris (p < 0.05)). Further studies are needed to confirm these preliminary results.