论新时期科技期刊青年编辑人才的创新素质

高素质的青年编辑人才是科技期刊发展的关键力量,其中创新素质起着举足轻重的作用。青年编辑在成长过程中,应该以正确理解科技期刊中的创新性和科技期刊编辑的刨新素质为前提,不断提高人文修养、注重知识的广度和深度、加强自身专业修养、加强主观能动性,从而达到提高创新素质的目的。     

Analysis of petal anthocyanins to investigate coloration mechanism in herbaceous peony cultivars

Petal coloration and anthocyanin compositions of 41 herbaceous peony cultivars were analyzed. Anthocyanins were identified by high-performance liquid chromatography–electrospray ionization-mass spectrometry (HPLC–ESI-MSⁿ) coupled with photodiode array detection (DAD). Peonidin-3,5-di-O-glucoside (Pn3G5G), pelargonidin-3,5-di-O-glucoside (Pg3G5G), cyanidin-3,5-di-O-glucoside (Cy3G5G), peonidin-3-O-glucoside (Pn3G), and cyanidin-3-O-glucoside (Cy3G) were the five major anthocyanins in herbaceous peony cultivars. Deep purple or reddish purple cultivars contained 4–5 anthocyanins, whereas pink cultivars only contained Cy3G5G and Pn3G5G, and their contents were much lower than those of purple cultivars. According to the chemical structures of three anthocyanidins in association with petal coloration, flowers were classified into three phenotypes: 1. “Pn, Cy, and Pg” (all purple flowers including two pink flowers); 2. “Pn, Cy” (pink flowers); 3 “Pn” (light pink and white flowers). The coloration mechanisms of cultivars with the pink and purple flowers were quite different. Correlations between lightness (L*) and chroma (C*), chromatic component a* and total anthocyanins (TA) value, a* and co-pigmentation index (CI) showed opposite tendencies, whereas L* and TA showed the same tendency in each group. High contents of Pn3G5G and Pg3G5G may responsible for the purple coloration of herbaceous peony cultivars.     

SH系矮化中间砧对‘富士’苹果树体生长、产量和果实品质的影响

2009年春季定植不同类型SH系矮化中间砧(SH1、SH3、SH6、SH9和SH40)苹果苗(宫藤富士/SH系砧木/平邑甜茶),株行距为1.5 m×5.0 m,纺锤形整形修剪,常规管理。2016年调查树体生长、冠层光照分布和果实产量品质的差异,为苹果矮化砧木的筛选利用提供参考。研究结果表明,栽植第8年(2016年),各中间砧嫁接树体的砧穗干周比无显著差异;SH1和SH6中间砧嫁接的树体最矮,SH40最高且冠幅最大;树体干周直径由高到低依次为:SH40、SH3、SH9、SH6和SH1;各中间砧嫁接树体总枝量均超过140万条·hm~(-2);SH6嫁接树体短枝比例最高,树势中庸;SH3和SH9短枝比例最低。各中间砧嫁接树体冠层平均相对光照强度由高到低依次为SH3(72.88%)、SH6(70.85%)、SH9(65.51%)、SH1(62.23%)和SH40(61.82%),SH6嫁接树体相对光照强度小于30%和40%的区域最小,仅为2.08%和4.17%。比较各中间砧树体连续稳产4年的结果情况,SH6嫁接树体4年(2013—2016年)累计产量最高且稳产性最好,SH1产量较低,SH9稳产性较差。各中间砧嫁接树平均单果质量由高到低依次为SH40 SH6 SH9 SH3 SH1。不同中间砧嫁接树果实不同类型糖含量和比例存在显著差异;而总酸含量、不同类型酸的含量(除草酸外)和比例差异不显著;SH6嫁接树果实糖酸比显著高于SH1、SH3和SH9。综合树体的生长和结果能力,SH6为中间砧嫁接‘富士’表现较好。     

Role of PI3K/Nrf2 signaling pathway in endotoxin-induced acute kidney injury in rabbits

AIM: To evaluate the role of phosphatidylinositol 3-kinase/nuclear factor E2-related factor 2 (PI3K/Nrf2) signaling pathway in endotoxin-induced acute kidney injury in rabbits. METHODS: Healthy male New Zealand white rabbits were randomly divided into 5 groups: control group (group C), LPS group (group L), wortmannin+LPS group (group WL), wortmannin group (group W) and dimthyl sulfoxide (DMSO) group (group D). Wortmannin at dose of 0.6 mg/kg was injected via the auricular vein in groups W and WL, DMSO at concentration of 0.08 mL/kg was injected in group D, while normal saline (0.08 mL/kg) was injected in groups C and L. LPS at dose of 5 mg/kg was injected via the auricular vein in groups L and WL 30 min later, and equal volume of normal saline was injected in group C, D and W for control. The rabbits were sacrificed 6 h after LPS or normal saline administration. The kidneys were removed for microscopic examination and the determination of histological scores of kidney (HSK). The concentrations of blood urea nitrogen (BUN) and creatinine (Cr), urinary α1-microglobulin (α1-MG), MDA content, SOD activity, the mRNA expression of Nrf2 and HO-1, and the protein levels of total Akt, p-Akt, total Nrf2, p-Nrf2, nuclear Nrf2 and HO-1 in the renal tissues were also detected. RESULTS: Compared with groups C, D and W, the concentrations of BUN and Cr, urinary α1-MG concentration, MDA content and HSK were significantly increased, while SOD activity was significantly decreased (P<0.05). The mRNA expression of Nrf2 and HO-1, and the protein levels of p-Akt, total Nrf2, p-Nrf2, nuclear Nrf2 and HO-1 in the renal tissues were significantly increased in groups L and WL. No significant change among groups C, D and W was observed. Compared with group L, the concentrations of BUN and Cr, urinary α1-MG concentration, MDA content and HSK were significantly increased, while SOD activity, the mRNA expression of Nrf2 and HO-1, and the protein levels of p-Akt, total Nrf2, p-Nrf2, nuclear Nrf2 and HO-1 in the renal tissues were significantly decreased in group WL. CONCLUSION: Activation of PI3K/Nrf2 signaling pathway may be one of the regulatory mechanisms of the body adapting to the endotoxin-induced acute kidney injury in rabbits.     

氮肥与行距对不同生育期虉草产量与品质的调控效应

以通草1号虉草(Phalaris arundinacea cv.Tongcao No.1)为供试材料,采用行距(30、50、70cm)和施氮量(含氮量为0、46、92kg·hm~(-2))二因素裂区试验设计,研究了行距与施氮量对不同生育时期虉草营养成分含量的影响。结果表明,氮肥与行距对虉草产量和品质影响明显。随施氮量增加,各生育时期虉草产量、粗蛋白、粗灰分、钙和磷含量升高,而无氮浸出物含量下降。抽穗期虉草粗纤维和酸性洗涤纤维含量随施氮量增加而降低,而成熟期和秋季再生期随施氮量增加反而升高。随着行距增加各生育时期虉草产量下降。在9个处理组合中,以行距30cm、施氮量92kg·hm~(-2)组合抽穗期和成熟期草产量最高,以行距70cm、施氮量92kg·hm~(-2)组合饲草品质最好。抽穗期是虉草刈割利用的适宜时期,种植行距30cm、施氮92kg·hm~(-2)时可获得较高的饲草产量和粗蛋白质产量。     

山东省临沂市动物产地检疫全过程痕迹化管理模式

为进一步做好动物产地检疫工作,临沂市经过近两年的探索,建立了"检疫申报大厅+全过程痕迹化管理"s相结合的产地检疫新模式,实现了产地检疫的可追溯及责任的落实与追究,规范了产地检疫程序,提升了动物检疫队伍的形象,保障了畜产品质量安全。     

Babesia gibsoni rhoptry-associated protein 1 and its potential use as a diagnostic antigen

A cDNA encoding the rhoptry-associated protein 1 (RAP-1) homologue was obtained by immunoscreening an expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 1740bp. Computer analysis suggested that the sequence contains an open reading frame of 1425bp encoding an expected protein with a molecular weight of 52kDa. Based on the sequence similarity, this putative protein was designated as the B. gibsoni RAP-1 (BgRAP-1). The BgRAP-1 gene was expressed in the Escherichia coli BL21 strain, and the recombinant BgRAP-1 was used as the antigen in the enzyme-linked immunosorbent assay (ELISA). The results can differentiate between the B. gibsoni-infected dog sera and the Babesia canis infected dog sera or the normal dog sera. Furthermore, the antibody response against the recombinant protein was maintained during the chronic stage of infection, indicating that the recombinant BgRAP-1 protein might be a useful diagnostic antigen for the detection of antibodies to B. gibsoni infection in dogs.     

浅谈“森林城”建设的总体规划

娄底市“森林城”是我国现阶段唯一的县（市）级“森林城”。通过对该市“森林城”的总体规划方法、建设的指导思想、规划原则、规划内容和范围等问题进行探讨,试图摸索出“森林城”建设总体规划的方法,为今后“森林城”建设提供参考。     

提高苏云金杆菌对棉铃虫毒力的研究

在含有Ｂｔ毒素的人工饲料中,通过加入碱性化合物提高其对昆虫中肠的作用,或加入蛋白酶激活剂、无机化合物和有机来提高Ｂｔ的毒力效价。试验结果表明,加入碳酸钙、硫酸锌、氯化镁、苹果酸、马来酸、甲酸和巯基乙醇酸钠,可使Ｂｔ毒力效价分别提高０．９５１．２２,０．３７,０．４７,１．１７,２．１０和９．０倍,其中加入硫酸锌、甲酸和巯基乙醇酸钠的增效效果较好。     

棉花gDNA体细胞染色体FISH技术

介绍了棉花基因组ＤＮＡ（ｇｅｎｏｍｅＤＮＡ,简为ｇＤＮＡ）体细胞染色体荧光原位杂交〔ＦＩＳＨ〕的技术流程,并着重分析和讨论了影响试验结果的关键因素,包括染色体和探针的变性条件、染色体的蛋白酶Ｋ处理技巧等。试验中作为靶ＤＮＡ的体细胞染色体采用棉属异源四倍体种海岛棉;探针和封阻均采用ｇＤＮＡ,材料是棉属二倍体种Ａ染色体组（Ａｇｅｎｏｍｅ）的棉种（亚洲棉和草棉）和Ｄ染色体组（Ｄｇｅｎｏｍｅ）的棉种（瑟伯氏棉、雷蒙德氏棉、戴维逊氏棉等）,分别交互使用。试验结果比较理想,获得良好的ＦＩＳＨ片子,而且重复性好。