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971.
972.
973.
新疆哈密瓜蔓枯病及防治研究 总被引:6,自引:0,他引:6
本文描述了哈密瓜蔓桔病两种症状类型。自然寄主主要是哈密瓜,1996年偶见轻微侵染西葫芦及籽西瓜。人工接种可侵染梨瓜及西瓜等葫芦科作物,惟对哈密瓜致病性强,对其它瓜类则弱。哈密瓜苗及田间成株接种试验表明,地面任何组织无论有伤或无伤都能侵染。该病主要借灌溉与风雨传播,病健蔓靠接也是一种传病方式。根据菌原形态及寄主范围等特点,明确本菌无性阶段为瓜壳单隔孢菌Ascochy-ts cucumis Fautr.et Roum.有性阶段为甜瓜球腔菌Mycosp-haerellamelonis (Pass) Chiu et J.C.Walker.防治研究表明.发病初期和以后每隔8-10天连续2-3次喷洒利克菌或拌种双都有很好的预防和治疗效果。 相似文献
974.
本项研究于2000年5月—2001年5月从内蒙古锡林郭勒盟地区,不同草原景现带牛、羊体表和草地采集草原血蝉1800只,用直接荧光抗体法检查蜱带菌情况,带菌率为7.5%(6/80)。晴视野显微镜检查蜱带菌情况,带菌率为2%(2/100);用间接荧光法进行血清流行病学调查,结果证实,调查点的牛和野鼠均存在莱姆病螺旋体感染,其感染率分别为10%和20%,都高于当地人群平均感染率(7.09%),上述研究证实内蒙古锡林郭勒盟地区人畜及野生动物均有莱姆病螺旋体感染。 相似文献
975.
本试验较系统地研究了黄花菜根尖细胞染色体的核型特征和带型特征,其中,对黄花菜G—带带型的研究,为国内首次报道。 相似文献
976.
Tang B Wang X Liang H Jia B Chen Z 《Journal of agricultural and food chemistry》2005,53(22):8452-8459
The beta-cyclodextrin-thiabendazole (beta-CD-TBZ) inclusion complex was synthesized and its structure characterized by (1)H NMR and IR. The mechanism of the supramolecular interaction of TBZ and beta-CD has been studied and discussed by spectrophotometry. The results showed that the phenyl ring of TBZ was included in the beta-CD cavity to form a 1:1 host-guest complex with an apparent formation constant of 1.60 x 10(3) mol(-1).L. On the basis of the enhancement of the absorbance of TBZ produced through complex formation, a spectrophotometric method for the determination of TBZ in bulk aqueous solution in the presence of beta-CD was developed. The linear relationship between the absorbance and TBZ concentration was obtained in the range of 8.86 x 10(-7)-1.45 x 10(-5) mol/L. The detection limit was 2.71 x 10(-7)mol/L, and the relative standard deviation was 0.86%. The interference of 48 coexisting substances was slight. The proposed method has been successfully applied to the determination of TBZ in fruits with recoveries of 96-103%. 相似文献
977.
Duck enteritis virus (DEV) is a herpesvirus that causes an acute, contagious, and fatal disease. In the present article, we introduce a quantitative real-time polymerase chain reaction (PCR) assay for DEV DNA using TaqMan technology and a two-step protocol. It was confirmed to be rapid, sensitive, and specific for DEV detection. The primers and probe were designed and directed to the DNA polymerase gene of DEV. The method will provide a valuable tool for rapid laboratory diagnosis of DEV infection. By virtue of its high-throughput format and its ability to accurately quantify the viral DNA, the method may be useful for large epidemiological surveys and clarification of pathogenesis, such as latency and reactivation of the virus. 相似文献
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979.
980.
Rice Pi-ta gene Confers Resistance to the Major Pathotypes of the Rice Blast Fungus in the United States 总被引:1,自引:0,他引:1
Jia Y Wang Z Fjellstrom RG Moldenhauer KA Azam MA Correll J Lee FN Xia Y Rutger JN 《Phytopathology》2004,94(3):296-301
ABSTRACT The Pi-ta gene in rice prevents the infection by Magnaporthe grisea strains containing the AVR-Pita avirulence gene. The presence of Pi-ta in rice cultivars was correlated completely with resistance to two major pathotypes, IB-49 and IC-17, common in the U.S. blast pathogen population. The inheritance of resistance to IC-17 was investigated further using a marker for the resistant Pi-ta allele in an F(2) population of 1,345 progeny from a cross of cv. Katy with experimental line RU9101001 possessing and lacking, respectively, the Pi-ta resistance gene. Resistance to IC-17 was conferred by a single dominant gene and Pi-ta was not detected in susceptible individuals. A second F(2) population of 377 individuals from a reciprocal cross between Katy and RU9101001 was used to verify the conclusion that resistance to IC-17 was conferred by a single dominant gene. In this cross, individuals resistant to IC-17 also were resistant to IB-49. The presence of Pi-ta and resistance to IB-49 also was correlated with additional crosses between 'Kaybonnet' and 'M-204', which also possess and lack Pi-ta, respectively. A pair of primers that specifically amplified a susceptible pi-ta allele was developed to verify the absence of Pi-ta. We suggest that Pi-ta is responsible for resistance to IB-49 and IC-17 and that both races contain AVR-Pita genes. 相似文献