Biosynthesis of 14C-phytoene from tomato cell suspension cultures (Lycopersicon esculentum) for utilization in prostate cancer cell culture studies

This work describes the development and utilization of a plant cell culture production approach to biosynthesize and radiolabel phytoene and phytofluene for prostate cancer cell culture studies. The herbicide norflurazon was added to established cell suspension cultures of tomato (Lycopersicon esculentum cv. VFNT cherry), to induce the biosynthesis and accumulation of the lycopene precursors, phytoene and phytofluene, in their natural isomeric forms (15-cis-phytoene and two cis-phytofluene isomers). Norflurazon concentrations, solvent carrier type and concentration, and duration of culture exposure to norflurazon were screened to optimize phytoene and phytofluene synthesis. Maximum yields of both phytoene and phytofluene were achieved after 7 days of treatment with 0.03 mg norflurazon/40 mL fresh medium, provided in 0.07% solvent carrier. Introduction of 14C-sucrose to the tomato cell culture medium enabled the production of 14C-labeled phytoene for subsequent prostate tumor cell uptake studies. In DU 145 prostate tumor cells, it was determined that 15-cis-phytoene and an oxidized product of phytoene were taken up and partially metabolized by the cells. The ability to biosynthesize, radiolabel, and isolate these carotenoids from tomato cell cultures is a novel, valuable methodology for further in vitro and in vivo investigations into the roles of phytoene and phytofluene in cancer chemoprevention.     

Controls on early post-fire woody plant colonization in riparian areas

Fire in riparian areas has the potential to influence the functions riparian vegetation provides to streams and aquatic biota. However, there is little information on the effects of fire on riparian areas. The objectives of the present study were to: (i) determine how fire severity interacts with riparian topographic setting, micro-environmental conditions, and pre-fire community composition to control post-fire regeneration; (ii) determine how riparian regeneration patterns and controls change during early succession; and (iii) determine how critical riparian functions are influenced by and recover after fire. Study locations included the Biscuit Fire in southwestern Oregon and the B&B Complex Fire in the Cascade Mountain Range of west-central Oregon, USA. We measured post-fire woody species regeneration, and measured factors such as fire severity, pre-fire species composition, and stream size as potential factors associated with post-fire regeneration patterns. At a relatively coarse spatial scale, patterns in post-fire colonization were influenced by elevation. At finer spatial scales, both conifer- and hardwood-dominated riparian plant communities were self-replacing, suggesting that each community type tends to occur in specific ecological settings. Abundant post-fire regeneration in riparian areas and the self-replacement of hardwood- and conifer-dominated communities indicate high resilience of these disturbance-adapted plant communities.     

The emergence and framing of farm-to-school initiatives: civic engagement, health and local agriculture

Interest in and initiation of farm-to-school (FTS) programs have increased in recent years, spurred on by converging public concerns about child obesity trends and risks associated with industrialization and distancing in the modern food system. A civic agriculture framework that more specifically considers civic engagement and problem solving offers insights about variations in the development and prospects for FTS programs. Drawing on comparative case studies of two emerging FTS initiatives in Pennsylvania—one in a rural setting and one in an urban setting—this article examines the role of internal and external “champions” in launching FTS programs and fostering civic engagement. Farm-to-school community stakeholders across the two cases framed FTS in broadly similar terms of (1) redressing poor food environments; (2) improving student nutrition, health and well-being; and (3) revitalizing rural community through support of local agriculture. However, specific concerns and emphases differed across the rural and urban cases, illustrating the significance of local context for such programs. The article concludes by discussing the importance of frame bridging and frame extension as strategies for expanding the FTS movement, and also ensuring programs that correspond to the specific circumstances and possibilities of their social and geographic settings.     

Protein adsorption on nano-scaled, rippled TiO2 and Si surfaces

We synthesized nano-scaled periodic ripple patterns on silicon and titanium dioxide (TiO(2)) surfaces by xenon ion irradiation, and performed adsorption experiments with human plasma fibrinogen (HPF) on such surfaces as a function of the ripple wavelength. Atomic force microscopy showed the adsorption of HPF in mostly globular conformation on crystalline and amorphous flat Si surfaces as well as on nano-structured Si with long ripple wavelengths. For short ripple wavelengths the proteins seem to adsorb in a stretched formation and align across or along the ripples. In contrast to that, the proteins adsorb in a globular assembly on flat and long-wavelength rippled TiO(2), but no adsorbed proteins could be observed on TiO(2) with short ripple wavelengths due to a decrease of the adsorption energy caused by surface curvature. Consequently, the adsorption behavior of HPF can be tuned on biomedically interesting materials by introducing a nano-sized morphology while not modifying the stoichiometry/chemistry.     

Health of farmed fish: its relation to fish welfare and its utility as welfare indicator

This brief review focuses on health and biological function as cornerstones of fish welfare. From the function-based point of view, good welfare is reflected in the ability of the animal to cope with infectious and non-infectious stressors, thereby maintaining homeostasis and good health, whereas stressful husbandry conditions and protracted suffering will lead to the loss of the coping ability and, thus, to impaired health. In the first part of the review, the physiological processes through which stressful husbandry conditions modulate health of farmed fish are examined. If fish are subjected to unfavourable husbandry conditions, the resulting disruption of internal homeostasis necessitates energy-demanding physiological adjustments (allostasis/acclimation). The ensuing energy drain leads to trade-offs with other energy-demanding processes such as the functioning of the primary epithelial barriers (gut, skin, gills) and the immune system. Understanding of the relation between husbandry conditions, allostatic responses and fish health provides the basis for the second theme developed in this review, the potential use of biological function and health parameters as operational welfare indicators (OWIs). Advantages of function- and health-related parameters are that they are relatively straightforward to recognize and to measure and are routinely monitored in most aquaculture units, thereby providing feasible tools to assess fish welfare under practical farming conditions. As the efforts to improve fish welfare and environmental sustainability lead to increasingly diverse solutions, in particular integrated production, it is imperative that we have objective OWIs to compare with other production forms, such as high-density aquaculture. However, to receive the necessary acceptance for legislation, more robust scientific backing of the health- and function-related OWIs is urgently needed.     

Converting conventional agriculture to poplar bioenergy crops: soil greenhouse gas flux

Conversion of agricultural fields to bioenergy crops can affect greenhouse gases (GHG) such as carbon dioxide (CO₂), methane (CH₄), and nitrous oxide (N₂O). Soil GHG emissions were measured seasonally in poplar bioenergy and agricultural fields at three Northwestern US locations. A forest stand was also used at one location for comparison. A portable gas analyzer was used to measure CO₂ efflux and CH₄ and N₂O fluxes were first measured with chambers and later with gradients. Agricultural soil had 17% larger CO₂ efflux rates than poplar soil. Chamber fluxes showed no differences in CH₄ uptake but did show higher N₂O fluxes in poplar than agricultural soil. Gradient CH₄ uptake rates were highest in agricultural soil in the summer but showed no N₂O flux differences. Forest soils had smaller quarterly CO₂ efflux rates than agricultural soils and greater CH₄ uptake rates than poplar soils. The largest GHG contributor to soil GHG flux was CO₂, with those being ～1000 times larger than CH₄ flux rates and ～500 times larger than N₂O flux rates based on CO₂ equivalences. Converting conventional agricultural cropland to poplar bioenergy production does not have adverse effects on soil greenhouse gas flux and these results could be useful for modeling or life cycle analysis of land use conversion.     

Promoting sustainable intensification in precision agriculture: review of decision support systems development and strategies

Precision agriculture provides important issues toward a more sustainable agriculture. Many farmers have the necessary technology to operate site-specifically, but they do not use it in practice, and thus available information and communications technology (ICT) systems are not used to their full potential. This paper addresses how to reduce the so-called “problem of implementation”, based on the knowledge that participatory approaches during the design and development process is one of the most important factors to frame technology adoption. The development of sustainable ICT systems through theories and methodologies from the fields of human computer interaction and user-centered design (UCD) is presented and an ongoing Swedish project for development of an agricultural decision support system (AgriDSS) for nitrogen fertilization is used as an example to frame the issue. The overreaching aim is to develop AgriDSSs that are sustainable in design as well as through design by stressing the importance of participatory approaches for the successful development of AgriDSSs. The Swedish project has the intention to apply a UCD approach, and some pitfalls on starting to use this way of working is identified as well as some suggestions on how to reduce them through co-learning processes. Despite the challenges presented in this paper, ICT can contribute significantly to long-term sustainable development. Thus, several competences and scientific disciplines need to act in concert to help develop a sustainable development of agriculture via a transdisciplinary approach that can make an impact on society at many levels.     

COMPARISON OF THORACIC ULTRASONOGRAPHY AND RADIOGRAPHY FOR THE DETECTION OF INDUCED SMALL VOLUME PNEUMOTHORAX IN THE HORSE

Small volume pneumothorax can be challenging to diagnose in horses. The current standard method for diagnosis is standing thoracic radiography. We hypothesized that thoracic ultrasonography would be more sensitive. Objectives of this prospective, experimental study were to describe a thoracic ultrasound method for detection of small volume pneumothorax in horses and to compare results of radiography and ultrasound in a sample of horses with induced small volume pneumothorax. Six mature healthy horses were recruited for this study. For each horse, five 50 ml air boluses were sequentially introduced via a teat cannula into the pleural space. Lateral thoracic radiographs and standardized ultrasound (2D and M‐mode) examinations of both hemithoraces were performed following administration of each 50 ml air bolus. Radiographs and ultrasound images/videos were analyzed for detection of pneumothorax by four independent investigators who were unaware of treatment status. Sensitivity, specificity, positive predictive values, negative predictive values, and agreement among investigators (Kappa test, κ) were calculated for radiography, 2D and M‐mode ultrasound. Comparisons were made using a chi‐squared exact test with significance set at P < 0.05. Two‐dimensional (84%) and M‐mode (80%) ultrasound were more sensitive than radiography (48%) for pneumothorax detection (P = 0.02 and P = 0.04, respectively). Specificity and positive predictive values were similar for all three imaging modalities (P = 1). Agreement between investigators for pneumothorax detection was excellent for 2D ultrasound (κ = 1), very good for M‐mode ultrasound (κ = 0.87), and good for radiography (κ = 0.79). Findings from this experimental study supported the use of thoracic ultrasonography as a diagnostic method for detecting pneumothorax in horses.     

Validation of a thermal threshold nociceptive model in bearded dragons (Pogona vitticeps)

Objectives

To validate a thermal threshold (TT) nociceptive model in bearded dragons (Pogona vitticeps) and to document TT changes after administration of morphine.