Measurement of 5-day biochemical oxygen demand without sample dilution or bacterial and nutrient enhancement

A direct method for measuring the 5-day biochemical oxygen demand (BOD₅) of aquaculture samples that does not require sample dilution or bacterial and nutrient enrichment was evaluated. The regression coefficient (R²) between the direct method and the standard method for the analyses of 32 samples from catfish ponds was 0.996. The slope of the regression line did not differ from 1.0 or the Y-intercept from 0.0 at P = 0.05. Thus, there was almost perfect agreement between the two methods. The control limits (three standard deviations of the mean) for a standard solution containing 15 mg/L each of glutamic acid and glucose were 17.4 and 20.4 mg/L. The precision of the two methods, based on eight replicate analyses of four pond water samples did not differ at P = 0.05.     

Bottom soil quality in Tilapia ponds of different age in Thailand

Bottom soil samples were collected from 35 ponds in the vicinity of Samutprakarn, Thailand. Ponds ranged in age from 3 to 39 years and had been used continuously for production of tilapia. Liming materials had been applied in large amounts, and bottom soils of all ponds had pH above 7, low exchange acidity, and free carbonate. Pond soils often contained between 1% and 2% total sulphur, suggesting that they were potential acid–sulphate soils. However, acidity from sulphide oxidation was not expressed because carbonate in the soil neutralized it. Concentrations of total carbon seldom exceeded 4% and the average for organic carbon was 1.90%. The correlations between pond age and both total carbon and organic carbon concentration were weak (r=0.34 and 0.36 respectively). Concentrations of nitrogen in bottom soils did not differ with pond age and ranged from 0.1% to 0.3% with an average of 0.19%. The average ratio of concentrations of carbon and nitrogen was 11. Acid‐extractable phosphorus concentrations averaged 217 mg kg^?1, but the phosphorus adsorption capacity averaged 768 mg kg^?1 suggesting that soils still have considerable reserve capacity to adsorb phosphorus. Ponds can be used annually for semi‐intensive production of tilapia, and presumably other species, for many years without serious deterioration of bottom soil quality.     

Changes in fatty acid and sterol composition during oogenesis in the pearl oyster Pinctada margaritifera

The fatty acid and sterol composition of the oyster Pinctada margaritifera during oogenesis and in eggs was analysed. No major differences were observed during oogenesis, but the egg composition was significantly different from that of gonads. The amount of saturated fatty acids was the highest in eggs and the C16:0 predominant (P<5%); by contrast, the amount of 22:6(n‐3) was significantly lower (P<5%) than in gonads. No major differences were observed for the polar lipid (PL) composition during oogenesis. The main free sterols in gonads and eggs were cholesterol and brassicasterol. Among free sterols, the proportion of cholesterol diminished continuously from the beginning to the end of gonad maturation, and this decrease persisted in eggs after spawning. Cholesterol represented 40% to 55% of the sterol ester encountered in gonad and eggs. This study allowed us to investigate the fatty acid and sterol composition during oogenesis of the pearl oyster P. margaritifera, leading to a clearer understanding of the nutritional requirements of pearl oyster during the reproduction process.     

Effects of Aeration, Alum Treatment, Liming, and Organic Matter Application on Phosphorus Exchange between Soil and Water in Aquaculture Ponds at Auburn, Alabama

Application of readily-oxidizable organic substrate to laboratory soil-water systems and fish ponds caused anaerobic conditions in bottom soil and water, and concentrations of soluble reactive phosphorus (SRP) increased. Aeration of ponds increased total phosphorus (TP) concentrations by suspending soil particles in the water, but SRP concentrations declined because of increased oxy- genation of bottom water and soil, Alum [Al₂(SO₄)₃·14H₂O] treatment of ponds reduced SRP and TP concentrations in ponds, but the low concentration of alum used, 20 mg/L, had little residual effect on phosphorus concentration. Application of agricultural limestone at 0.2 kg/m² to ponds with soil pH of 5.5 and Ca²⁺ concentration of 5 mg/L did not affect SRP and TP concentration. Unless pond soils were anaerobic at their surfaces, a condition not acceptable in thermally-unstratified fish ponds, soils released little phosphorus to the water. Strong adsorption of phosphorus by soils in intensive ponds with feeding is beneficial, because removal of phosphorus by aerobic soils is a control on excessive phytoplankton growth. In fertilized ponds, phosphorus must be applied at frequent intervals to replace phosphorus removed from the water by soils.     

Classification of gilthead sea bream (Sparus aurata) from 1H NMR lipid profiling combined with principal component and linear discriminant analysis

The combination of (1)H NMR fingerprinting of lipids from gilthead sea bream (Sparus aurata) with nonsupervised and supervised multivariate analysis was applied to differentiate wild and farmed fish and to classify farmed specimen according to their areas of production belonging to the Mediterranean basin. Principal component analysis (PCA) applied on processed (1)H NMR profiles made a clear distinction between wild and farmed samples. Linear discriminant analysis (LDA) allowed classification of samples according to the geographic origin, as well as for the wild and farmed status using both PCA scores and NMR data as variables. Variable selection for LDA was achieved with forward selection (stepwise) with a predefined 5% error level. The methods allowed the classification of 100% of the samples according to their wild and farmed status and 85-97% to geographic origin. Probabilistic neural network (PNN) analyses provided complementary means for the successful discrimination among classes investigated.     

Fine-earth translocation by tillage in stony soils in the Guadalentin, south-east Spain: an investigation using caesium-134

Tillage erosion is increasingly recognised as an important soil erosion process on agricultural land. In view of its potential significance, there is a clear need to broaden the experimental database for the magnitude of tillage erosion to include a range of tillage implements and agricultural environments. The study discussed in this paper sought to address the need for such data by examining tillage erosion by a duckfoot chisel plough in stony soils on steep slopes in a semi-arid environment. Results of the investigation of coarse fraction (rock fragment) translocation by tillage in this environment have been presented elsewhere and the paper focuses on tillage translocation and erosion of the fine earth. Tillage translocation was measured at 10 sites, representing both upslope and downslope tillage by a duckfoot chisel plough on five different slopes, with tangents ranging from 0.02 to 0.41. A fine-earth tracer, comprising fine earth labelled with ¹³⁴Cs, was introduced into the plough layer before tillage. After a single pass of the plough, incremental samples of plough soil were excavated and sieved to separate the fine earth from the rock fragments. Translocation of the fine-earth tracer was established by analysing the ¹³⁴Cs content of the samples of fine earth. These data were used to establish translocation distances for each combination of slope and tillage direction. Translocation distances of the fine earth were not significantly different from translocation distances of the coarse fraction. For all sites, except uphill on the 0.41 slope, translocation distances were found to be linearly related to slope tangent. The soil flux due to tillage for each site was calculated using the translocation distance and the mass per unit area of the plough layer. For slopes with tangents <0.25, the relationship between soil flux and tangent was linear and the soil flux coefficient derived was 520–660 kg m⁻¹ per pass. This is much larger than the coefficients found in other studies and this high magnitude is attributed to the non-cohesive nature and high rock fragment content of the soil in this investigation. A second contrast with previous studies was found in non-linearity in the relationship between soil flux and tangent when steeper slopes were included. This was a product of variation in plough depth between the steepest slopes and the remainder of the study area. On the basis of the study it is suggested that an improved understanding of tillage erosion may be obtained by considering the dual processes of tillage detachment (mass per unit area of soil subject to tillage) and tillage displacement (equivalent to translocation distance per pass) in assessing, comparing and modelling tillage translocation. An improved model is proposed that recognises the complexity of soil redistribution by tillage, provides a framework for process-based investigation of the controls on tillage fluxes, and allows identification of potential self-limiting conditions for tillage erosion.     

Effects of substrate sterilization,fungicide treatment,and mycorrhization helper bacteria on ectomycorrhizal formation of pedunculate oak (Quercus robur) inoculated with Laccaria laccata in two peat bare-root nurseries

Summary Pedunculate oak seedlings (Quercus robur) inoculated with the ectomycorrhizal fungus Laccaria lacata were grown for 1 year on fertilized sphagnum peat in two nurseries. Three factors affecting microbial populations in the substrate were studied, fungicide treatment of the seeds, peat disinfection before sowing (methyl bromide or steam pasteurization), and inoculation with mycorrhization helper bacteria. Treatment of acorns with Iprodione had no depressive effect on mycorrhiza formation. Both disinfection techniques were equivalent, stimulating or depressing mycorrhiza formation depending on the initial microflora in the peat. The introduction of two previously selected mycorrhization helper bacteria (one Pseudomonas fluorescens and one unidentified fluorescent pseudomonad), isolated from L. laccata sporocarps associated with Douglas fir—L. laccata ectomycorrhizas in other nurseries, significantly increased the mycorrhizal rate from 30 to 53% of the short roots. The implications of these results for the controlled mycorrhization of planting stocks and the specificity of mycorrhization helper bacteria are discussed.     

Whitened kernel surface: A fast and reliable method for assessing Fusarium severity on cereal grains by digital picture analysis

Fusarium head blight (FHB) is a cereal disease of major importance responsible for yield losses and mycotoxin contaminations in grains. Here, we introduce a new measurement approach to quantify FHB severity on grains based on the evaluation of the whitened kernel surface (WKS) using digital image analysis. The applicability of WKS was assessed on two bread wheat and one triticale grain sample sets (265 samples). Pearson correlation coefficients between Fusarium‐damaged kernels (FDK) and WKS range from r = 0.77 to r = 0.81 and from r = 0.61 to r = 0.86 for the correlation between deoxynivalenol (DON) content and WKS. This new scoring method facilitates fast and reliable assessment of the resistance to kernel infection and shows significant correlation with mycotoxin content. WKS can be automated and does not suffer from the “human factor” inherent to visual scorings. As a low‐cost and fast approach, this method appears particularly attractive for breeding and genetic analysis of FHB resistance where typically large numbers of experimental lines need to be evaluated, and for which WKS is suggested as an alternative to visual FDK scorings.     

Vectorizing agrochemicals: enhancing bioavailability via carrier‐mediated transport

Systemicity of agrochemicals is an advantageous property for controlling phloem sucking insects, as well as pathogens and pests not accessible to contact products. After the penetration of the cuticle, the plasma membrane constitutes the main barrier to the entry of an agrochemical into the sap flow. The current strategy for developing systemic agrochemicals is to optimize the physicochemical properties of the molecules so that they can cross the plasma membrane by simple diffusion or ion trapping mechanisms. The main problem with current systemic compounds is that they move everywhere within the plant, and this non‐controlled mobility results in the contamination of the plant parts consumed by vertebrates and pollinators. To achieve the site‐targeted distribution of agrochemicals, a carrier‐mediated propesticide strategy is proposed in this review. After conjugating a non‐systemic agrochemical with a nutrient (α‐amino acids or sugars), the resulting conjugate may be actively transported across the plasma membrane by nutrient‐specific carriers. By applying this strategy, non‐systemic active ingredients are expected to be delivered into the target organs of young plants, thus avoiding or minimizing subsequent undesirable redistribution. The development of this innovative strategy presents many challenges, but opens up a wide range of exciting possibilities. © 2018 Society of Chemical Industry     

Alkalinity and Hardness: Critical but Elusive Concepts in Aquaculture

Total alkalinity and total hardness are familiar variables in aquatic animal production. Aquaculturists—both scientists and practitioners alike—have some understanding of the two variables and of methods for adjusting their concentrations. The chemistry and the biological effects of alkalinity and hardness, however, are more complex than generally realized or depicted in the aquaculture literature. Moreover, the discussions of alkalinity and hardness—alkalinity in particular—found in water chemistry texts are presented in a rigorous manner and without explanation of how the two variables relate to aquaculture. This review provides a thorough but less rigorous discussion of alkalinity and hardness specifically oriented toward aquaculture. Alkalinity and hardness are defined, their sources identified, and analytical methods explained. This is followed by a discussion of the roles of the two variables in aquaculture, including their relationships with carbon dioxide, pH, atmospheric pollution, ammonia, and other inorganic nitrogen compounds, phytoplankton communities, trace metals, animal physiology, and clay turbidity. Liming and other practices to manage alkalinity and hardness are explained. Changes in alkalinity and hardness concentrations that occur over time in aquaculture systems are discussed. Emphasis is placed on interactions among alkalinity, hardness, water quality, and aquacultural production.