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151.
Smith DJ Lapedes AS de Jong JC Bestebroer TM Rimmelzwaan GF Osterhaus AD Fouchier RA 《Science (New York, N.Y.)》2004,305(5682):371-376
The antigenic evolution of influenza A (H3N2) virus was quantified and visualized from its introduction into humans in 1968 to 2003. Although there was remarkable correspondence between antigenic and genetic evolution, significant differences were observed: Antigenic evolution was more punctuated than genetic evolution, and genetic change sometimes had a disproportionately large antigenic effect. The method readily allows monitoring of antigenic differences among vaccine and circulating strains and thus estimation of the effects of vaccination. Further, this approach offers a route to predicting the relative success of emerging strains, which could be achieved by quantifying the combined effects of population level immune escape and viral fitness on strain evolution. 相似文献
152.
Hillenmeyer ME Fung E Wildenhain J Pierce SE Hoon S Lee W Proctor M St Onge RP Tyers M Koller D Altman RB Davis RW Nislow C Giaever G 《Science (New York, N.Y.)》2008,320(5874):362-365
Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes ( approximately 80%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole-genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition. 相似文献
153.
阐述了南京农业大学与连云港实施的“百名教授科教兴百村小康工程”活动,此项活动探索了建立农业高校服务“三农”长效机制,科技专家与农村基层单位长期挂钩结对为“三农”服务的新途径.找准了教学科研、成果转化与地方经济建设的结合点,促进了不发达地区农业科技进步、农村经济发展和农民收入增加。 相似文献
154.
The ability to select a better option from multiple acceptable ones is important for animals to optimize their resources. The mechanisms that underlie such decision-making processes are not well understood. We found that selection of egg-laying site in Drosophila melanogaster is a suitable system to probe the neural circuit that governs simple decision-making processes. First, Drosophila females pursue active probing of the environment before depositing each egg, apparently to evaluate site quality for every egg. Second, Drosophila females can either accept or reject a sucrose-containing medium, depending on the context. Last, communication of the "acceptability" of the sucrose-containing medium as an egg-laying option to the reproductive system depends on the function of a group of insulin-like peptide 7 (ILP7)-producing neurons. These findings suggest that selection of egg-laying site involves a simple decision-making process and provide an entry point toward a systematic dissection of this process. 相似文献
155.
Soft tissues are preserved within hindlimb elements of Tyrannosaurus rex (Museum of the Rockies specimen 1125). Removal of the mineral phase reveals transparent, flexible, hollow blood vessels containing small round microstructures that can be expressed from the vessels into solution. Some regions of the demineralized bone matrix are highly fibrous, and the matrix possesses elasticity and resilience. Three populations of microstructures have cell-like morphology. Thus, some dinosaurian soft tissues may retain some of their original flexibility, elasticity, and resilience. 相似文献
156.
Morin S Savarino J Frey MM Yan N Bekki S Bottenheim JW Martins JM 《Science (New York, N.Y.)》2008,322(5902):730-732
Atmospheric nitrogen oxides (NOx =NO+ NO2) play a pivotal role in the cycling of reactive nitrogen (ultimately deposited as nitrate) and the oxidative capacity of the atmosphere. Combined measurements of nitrogen and oxygen stable isotope ratios of nitrate collected in the Arctic atmosphere were used to infer the origin and fate of NOx and nitrate on a seasonal basis. In spring, photochemically driven emissions of reactive nitrogen from the snowpack into the atmosphere make local oxidation of NOx by bromine oxide the major contributor to the nitrate budget. The comprehensive isotopic composition of nitrate provides strong constraints on the relative importance of the key atmospheric oxidants in the present atmosphere, with the potential for extension into the past using ice cores. 相似文献
157.
A physical map of the 1-gigabase bread wheat chromosome 3B 总被引:2,自引:0,他引:2
Paux E Sourdille P Salse J Saintenac C Choulet F Leroy P Korol A Michalak M Kianian S Spielmeyer W Lagudah E Somers D Kilian A Alaux M Vautrin S Bergès H Eversole K Appels R Safar J Simkova H Dolezel J Bernard M Feuillet C 《Science (New York, N.Y.)》2008,322(5898):101-104
As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence-based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17-billion-base-pair hexaploid bread wheat genome (2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome (BAC)-based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome-specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach. 相似文献
158.
Refojo D Schweizer M Kuehne C Ehrenberg S Thoeringer C Vogl AM Dedic N Schumacher M von Wolff G Avrabos C Touma C Engblom D Schütz G Nave KA Eder M Wotjak CT Sillaber I Holsboer F Wurst W Deussing JM 《Science (New York, N.Y.)》2011,333(6051):1903-1907
The corticotropin-releasing hormone receptor 1 (CRHR1) critically controls behavioral adaptation to stress and is causally linked to emotional disorders. Using neurochemical and genetic tools, we determined that CRHR1 is expressed in forebrain glutamatergic and γ-aminobutyric acid-containing (GABAergic) neurons as well as in midbrain dopaminergic neurons. Via specific CRHR1 deletions in glutamatergic, GABAergic, dopaminergic, and serotonergic cells, we found that the lack of CRHR1 in forebrain glutamatergic circuits reduces anxiety and impairs neurotransmission in the amygdala and hippocampus. Selective deletion of CRHR1 in midbrain dopaminergic neurons increases anxiety-like behavior and reduces dopamine release in the prefrontal cortex. These results define a bidirectional model for the role of CRHR1 in anxiety and suggest that an imbalance between CRHR1-controlled anxiogenic glutamatergic and anxiolytic dopaminergic systems might lead to emotional disorders. 相似文献
159.
Marilyn Holly Carla A. Kirts Yvonne Everett Jan L. Flora David O. Hansen Cornelia Butler Flora John A. Hayward David Haenke Alessandro Bonanno 《Agriculture and Human Values》1988,5(4):57-78
Marilyn Holly is an Associate Professor of Philosophy at the University of Florida. Her special interests include political philosophy, ethics and ecology, Jungian thought, and Native American spirituality in its relation to the environment. She is currently at work on a monograph-length piece on the latter topic. She has taught an undergraduate course in ethics and ecology, and plans to do so again. The following essay emerged from her search for a suitable text for such a course. 相似文献
160.
Sequence of a probable potassium channel component encoded at Shaker locus of Drosophila 总被引:31,自引:0,他引:31
B L Tempel D M Papazian T L Schwarz Y N Jan L Y Jan 《Science (New York, N.Y.)》1987,237(4816):770-775
Potassium currents are crucial for the repolarization of electrically excitable membranes, a role that makes potassium channels a target for physiological modifications that alter synaptic efficacy. The Shaker locus of Drosophila is thought to encode a K+ channel. The sequence of two complementary DNA clones from the Shaker locus is reported here. The sequence predicts an integral membrane protein of 70,200 daltons containing seven potential membrane-spanning sequences. In addition, the predicted protein is homologous to the vertebrate sodium channel in a region previously proposed to be involved in the voltage-dependent activation of the Na+ channel. These results support the hypothesis that Shaker encodes a structural component of a voltage-dependent K+ channel and suggest a conserved mechanism for voltage activation. 相似文献