一个马铃薯Y病毒山东分离物的分离与鉴定

 从具有典型花叶症状的马铃薯叶片中分离到马铃薯Y病毒(Potato virus Y,PVY)(本文称PVY-SD-TA分离物),扩繁后,提纯病毒,电镜下可观察到700~900 nm×11 nm的病毒粒体,病组织超薄切片观察可见风轮状的内含体结构,寄主反应特性研究表明其能侵染2科13种植物。SDS-PAGE电泳检测病毒编码的外壳蛋白亚基的分子量为33 kDa。以PVY-SD-TA基因组RNA为模板,应用RT-PCR方法和特异引物合成了外壳蛋白基因。对cDNA全序列分析表明,PVY-SD-TA CP基因核苷酸序列与N株系的同源性为96%,与GenBank中登录序列号为AJ390306的O株系分离物的同源性最高,为99%;与国内不同学者报道的PVY中国流行株的同源性分别为96%,97%和98%。通过以上生物学特性和分子水平的研究将PVY-SD-TA鉴定为普通株系(PVY^O株系)。     

淡紫拟青霉菌料防治大豆胞囊线虫的后效研究

淡紫拟青霉不同菌株的培养菌料，施２２５ｋｇ／ｈｍ２，对大豆胞囊线虫不但当年有４５％～６９％的较好防效，而且第２年和第３年仍然有２２．５％～３７．７％和７．３％～２２．９％的后效，即空胞囊和被寄生的胞囊数量增加。     

燕麦枯对环境生物的安全性评价

本文报道了杂环类内吸性除草剂燕麦枯（ｄｉｆｅｎｚｏｑｕａｔ）对家蚕，蜜蜂，广赤眼蜂，瓢虫，蚯蚓及土壤微生物种群的生物活性测定结果，该结果表明，燕麦枯在高于使用剂量下对供试的生物种群的没有明显影响，由此推断，在推荐用量下燕麦枯不会造成对田间生物种群的破坏。     

以甜菜粕取代小麦的全混合颗粒饲料对羔羊瘤胃pH值的影响

本试验选用24只3-3.5月龄的波德代(♂)×蒙古羊(♀)和陶赛特(♂6)×蒙古羊(♀)杂交一代羔羊,按2×2×2因子设计,研究了两个营养水平下以高果胶的甜菜粕取代小麦的全混合颗粒饲料对两种羔羊瘤胃pH值的影响。结果表明,低营养水平组的pH值显著高于高营养水平组(P=0.015);食后4 h(P-0.019)、6 h(P=0.009)的pH值在营养水平和饲料间存在互作,高营养水平组,小麦组羔羊的瘤胃液pH高于甜菜粕组,而低营养水平组、甜菜粕组的pH高于小麦组;羊品种对pH值无影响。     

温度对杨树菇栽培性状影响的统计学分析

分析了温度环境因子对杨树菇生长发育的影响 ,研究结果表明 :菌丝体营养生长以 2 5～ 2 7℃为最适。原基分化温度 11～ 16℃ ,子实体发育以 13～ 2 6℃最适 ,不同菌株的最适温度存在差异。通过对日平均气温与杨树菇生产周期和产量、品质的关系试验 ,温度与病害发生关系试验 ,用统计分析方法得出了杨树菇各生理阶段的最适温度范围 ,为杨树菇的优质高产栽培提供了温度环境因子的基础数据和调控依据。     

葡萄品种浆果成熟期多样性及归类标准评价

对郑州葡萄圃内260个鲜食和153个酿酒品种的浆果成熟期进行了鉴定评价。在郑州地区,最早熟的葡萄种质材料早莎巴珍珠6月29日生理成熟,果实发育期48d;最晚熟的种质材料大宝10月12日成熟,果实发育期144d,在其间的103d时间里不断有成熟的葡萄品种,表现了丰富的多样性。酿酒葡萄的果实成熟期相对集中,大多数酿酒葡萄品种在8月中旬成熟,7月份和9月份成熟的葡萄品种所占的比例极少。果实发育期≤60d、61～80d、81～100d、101～120d、≥121d的品种次数呈正态分布,果实发育期81～100d的品种占收集品种的53.03%;果实发育期少于60d的极早熟品种和果实发育期多于120d的极晚熟品种所占比例较少。早、中、晚熟品种按照浆果发育期进行归类较为简便。     

农药酶免疫分析技术研究进展

自从酶免疫分析原理提出后，近30年来已获得了巨大的进步，成为农药分析的一个非常重要的方法。本文对农药酶免疫分析技术的原理和测定程序进行了评述．并总结了近年酶免疫分析在农药领域研究中的应用，最后，对农药酶免疫分析技术存在的问题和发展趋势作了讨论。     

Relationship of p21WAF1 gene polymorphisms with protein expression in breast carcinomas

AIM: To investigate the relationship between p21^WAF1gene polymorphisms and protein expression in breast carcinoma. METHODS: Polymerase chain reaction single-strand conformation polymorphisms technique (PCR-SSCP) and immunohistochemical assay of S-P immunostaining technique were used to study polymorphisms of p21^WAF1 and protein expression respectively on the specimen of paraffin-embedded tissues in 100 cases of breast carcinomas and 40 benign breast diseases as control. RESULTS: Two p21^WAF1 gene polymorphisms were found in 18% (18/100) of breast carcinomas and 5% (2/40) of control samples. The difference between the two groups was statistically significant (χ²=3.94, P＜0.05). The positive immunohistochemical reaction of p21^WAF1 protein were found in 50% (50/100) of breast carcinomas and 12.5% (5/40) of control samples. The difference between the two groups was statistically significant (χ²=16.84, P＜0.01). The positive immunohistochemical reaction of p21^WAF1 protein were found in 100% (18/18) of breast carcinomas with p21^WAF1 gene polymorphisms and 39% (32/82) of no p21^WAF1 gene polymorphisms. The difference between two groups was statistically significant (χ²=21.95, P＜0.01). The p21^WAF1 gene polymorphisms were correlated with the protein expression in breast carcinomas (r=0.576, P＜0.01). CONCLUSION: p21^WAF1 gene polymorphisms may create the different copies of mRNA and may make relevant protein molecules.     

Differential regulating effect on estrogen receptor α and β expression in female rat hippocampus and cortex regions between different types of estrogen regents

AIM: To study the regulatory effect two different estrogen reagents on expressions of estrogen receptor α and β in female rat hippocampus and cortex regions. METHODS: 12 cycles after ovariectomy, female rats were orally injected with premarin or progynova for 3 cycles before sacrificed. Semiquantitative RT-PCR was used to detect the ERs mRNA expression and SP immunohistochemistry was performed to measure the ERs protein distribution and expression. RESULTS: In premarin group, ER α mRNA levels in both hippocampus and cortex tissues decreased significantly compared with control. ER α protein level in hippocampus was lower than that in the control. However, ER α protein level in cortex had no statistical difference. ER β mRNA in the two regions and ER β protein in cortex had no statistical differences compared with control, while ER β protein level in hippocampus was higher than that in the control. In progynova group, both mRNA and protein levels of ER β increased significantly in the two regions compared with the control, and ER α mRNA level also increased in hippocampus, but ER α mRNA level in cortex and ER α protein levels in the above two regions showed no statistical differences. CONCLUSION: There were differential regulatory effects on ER α and ER β expression in female rat cognitive regions between the two different types of estrogen reagents, which may be one of the mechanisms of varied effects in different estrogen replacement therapy reagents.     

Angiopoietin-1 and regulation of vascular permeability

Angiopoietin-1 (Ang-1) is a newly-found endothelium-specific proangiogenic factor and it had been proved essential roles in both vasculogenesis and angiogensis. Among them, its anti-leakage ability may have great potential applications in clinical treatment of vascular hyper-permeability in a variety of diseases such as cancer, diabetic retinopathy, rheumatoid arthritis, asthma. In this review, some research progresses focused on this aspect are discussed.