利用同位素~(32)P示踪技术测定西伯利亚红松接穗与砧木适应性和亲和性的研究

利用同位素32P示踪技术,研究嫁接后西伯利亚红松树体内营养的分配、吸收与运输情况,评价接穗与砧木适应性和亲和性。结果表明:嫁接树的营养吸收明显大于非嫁接树,油松-西伯利亚红松嫁接树营养物质交换最好,是适应性较强的组合,这与引种组长期嫁接试验的生长势观察记录相一致。     

Effect of rhynchophylline on TGF-β1/Smad pathway for processing ventricular remodeling in spontaneously hypertensive rats

AIM: To investigate the effect of rhynchophylline (Rhy) on blood pressure, cardiac hypertrophy and myocardial fibrosis in spontaneously hypertensive rats (SHR). METHODS: Spontaneously hypertensive rats were randomly divided into model group, high dose (10 mg·kg^-1·d^-1) and low dose (2.5 mg·kg^-1·d^-1) group of rhynchophylline, captopril group (17.5 mg·kg^-1·d^-1). Wistar-Kyoto rats were used as normal control. Respectively, systolic blood pressure was measured by tail cuff every 2 weeks. After 10 weeks, heart weight index and left ventricular weight index were calculated. The myocardial hydroxyproline and plasma angiotensin Ⅱ were detected. Moreover, basic myocardial histopathological changes and myocardial collagen fibres were observed by HE staining and Masson staining, respectively. The protein expression of TGF-β₁ and Smad3 in the myocardium was measured by the methods of immunohistochemistry and Western blot. RESULTS: Compared with SHR model group, Rhy significantly reduced blood pressure (P<0.05), the levels of HYP in the myocardium (P<0.05) and the levels of AngⅡ in the plasma (P<0.01). The pathological damages of the myocardial tissues and collagen deposition were attenuated. The protein expression of TGF-β₁ and Smad3 was significantly reduced by the treatment with Rhy (P<0.01). CONCLUSION: Rhynchophylline reduces blood pressure and adjusts to improve ventricular remodeling of SHR. The mechanism may be involved in the TGF-β₁/Smad pathway and reducing AngⅡ content.     

Expression of Jagged2/Notch3 signaling molecules in pulmonary hypertensive rats induced by monocrotaline

AIM: To study the expression of Jagged2/Notch3 signaling molecules in pulmonary vascular wall of pulmonary hypertensive rats induced by monocrotaline. METHODS: SD rats were randomly divided into normal control group (C group,n=15), solvent control group (S group,n=15) and monocrotaline model groups (M group,n=15). The model of pulmonary hypertension was established by a single subcutaneous injection of monocrotaline (50 mg/kg). The rats in S group were given a single subcutaneous injection of the same dose of solvent. After 4 weeks, the pulmonary vascular remodeling was assessed by HE staining, and the mean pulmonary artery pressure (mPAP) and right ventricular systolic pressure (RVSP) were determined by right heart catheterization. The expression of Jagged2/Notch3/Hes5 molecules in the pulmonary vascular wall was detected by immunohistochemical method and real-time PCR. RESULTS: Compared with S group and C group, the percentage of medial wall thickness of smaller arteries in model group increased significantly (P<0.01). The levels of mPAP and RVSP in M group were significantly higher than those in S group and C groups (P<0.01). The results of real-time PCR showed that the expression of Jagged2, Notch3 and Hes5 was significantly increased in M group compared with S group and C group. The data from immunohistochemical detection indicated that Jagged2 mainly expressed in the intima of small lung artery, Notch3 and Hes5 mainly expressed in the medial smooth muscle cells. Compared with S group and C group, the expression of Jagged2 and Notch3 was significantly increased in the lung small arteries of M group. CONCLUSION: The activation of Jagged2/Notch3 signaling pathway might play an important role in the formation of pulmonary hypertension.     

红景天苷提取工艺研究进展

红景天苷是景天科红景天属植物中广泛存在的酚类化合物,具有抗缺氧、抗辐射以及抗癌等药理作用,可用于一些心血管疾病和肝病的预防和治疗。同时红景天苷也可用作保健食品,具有滋补强身、抗氧化、抗疲劳、提高体力、延缓肌体衰老等功能。现对红景天属植物中红景天苷的提取工艺进行了综述,并简要阐述了各方法的优缺点,以期为深入研究红景天苷提取工艺提供理论基础。     

盐胁迫下沙柳幼苗的生理生化变化

以沙柳幼苗为试材,研究了0(对照)、50、100、150mM NaCl胁迫对沙柳幼苗的形态变化、酶液宏观变化、酶活性变化及内源激素含量变化的影响。结果表明:100mM NaCl盐浓度及以下胁迫对沙柳幼苗近乎无影响,ABA、IAA、GA的含量相对平稳,无太大变化,通过测定内源激素的含量可发现,100mM NaCl盐浓度时沙柳幼苗长势出现下降,100mM NaCl盐浓度以上的胁迫对沙柳幼苗的影响显著,沙柳幼苗所能承受NaCl迫害的最高Na盐浓度处于100mM NaCl和150mM NaCl之间。150mM NaCl盐浓度胁迫时,其在第1天和第3天间代谢紊乱程度达到其可耐受的极限程度,随后的几天植株走向死亡。同时沙柳幼苗在濒临死亡时其ABA含量无大幅度升高,这与常见植物有所不同。而IAA、GA的含量呈现出在短时间内迅速达到最大含量,随后的几天内含量有所降低。在极高浓度的NaCl胁迫下,沙柳幼苗的SOD活性在短时间内迅速下降后持续大幅度上升,沙柳幼苗死亡时,SOD活性达到最高。沙柳幼苗受到NaCl胁迫时,其CAT比SOD和POD敏感,CAT活性受到的影响更显著。     

施氮量对水稻主要米质性状及RVA谱特征参数的影响

在盆栽试验条件下，以汕优63、武育粳3号为试验材料，研究水稻移栽后的施氮量对主要米质性状和RVA谱特征参数的影响。结果表明：整精米率、粗蛋白含量均随施氮量的增加而提高；垩白粒率、垩白度对供氮水平的反应因品种而异；胶稠度随施氮量的提高两者都变软；直链淀粉含量、糊化温度对氮素反应不敏感，处理间无显著差异。稻米     

灌水量对大麦籽粒蛋白质及其组分含量的影响

研究在大田条件下灌水量对大麦籽粒蛋白质及其组分含量的影响。结果表明:不同灌水量对大麦籽粒蛋白质性状的效应不明显;品种与灌水量的互作对大麦籽粒醇溶蛋白的作用达到显著水平;不同品种间在蛋白质及其各组分含量上均有不同程度的差异。说明大麦籽粒蛋白质性状的水分生理不敏感,对水分条件的适应性较强。     

The GDSS Framework of Organization-Oriented Dynamic Alliance Based on Multi-Agent

The important strategy decision making system, one of the difficult problems of the research of Group Decision Support System, is an open complex huge system, which is almost non structure and hard to construct. In order to organize and take advantage of decision making departments more effectively, this paper presents the thought of combining Multi Agent System with Dynamical Alliance and set up the GDSS Framework of Organization Oriented Dynamic Alliance based on Multi Agent after analyzing the characteristics of MAS and Dynamical Alliance. Different departments can be organized more effectively on the three layer system structure composed of the strategy layer, the tactics layer and the operation layer. The decision making layer of each department is composed of the integrate decision making subsystem, which means that the decision making layer of each department can be used solely or be a part of Dynamic Alliance to make the important strategy decision and the system gets more flexible.     

松土促根剂对土壤质地、冬小麦产量和淀粉糊化特性的影响

为了解决近年来华北地区麦田因多年旋耕导致土壤耕层过浅、土壤质地变差、小麦根系发育不良以及小麦产量品质降低等问题,在平衡配方施肥的基础上,通过田间试验研究了Agri-star松土促根剂对土壤质地及小麦产量和淀粉糊化特性的影响。结果表明,施用松土促根剂和土壤深耕处理均可起到降低土壤容重,增加单位体积小麦根系质量,改善土壤理化性状的作用。此外,施用松土促根剂能够提高冬小麦成穗数和穗粒数,进而起到提高小麦籽粒产量的效果,其中以1号松土促根剂+配方肥处理籽粒产量最高,平均达7 925 kg/hm2,较单施配方肥处理增产12.9%。研究还发现,施用配方肥、添加松土促根剂或者进行土壤深耕处理能够改善小麦籽粒淀粉品质,同样也以1号松土促根剂+配方肥处理效果最好。综合比较分析,在当前华北地区免耕、旋耕麦田,应用Agri-star松土促根剂可以改善土壤质地,提高小麦产量和改善小麦籽粒淀粉品质,具有广阔的应用前景。     

太行山区苹果园昆虫群落及其动态变化研究

研究了河北太行山中南部苹果园昆虫群落的组成、发展演替、群落的空间、时间格局;揭示了苹果生长季节昆虫群落结构的变动规律,确定了不同时期、不同部位的害虫优势种;明确了苹果园昆虫多样性、均匀度和优势度参数变化较大,在太行山区苹果园生态系统中,仅靠天敌的作用,不能起到控制害虫危害目的,需以人工干预。