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71.
本研究采用A ndersen-6级空气微生物样品收集器,选用血-葡萄糖-琼脂培养基为采样介质,对两个不同种兔舍环境空气中需氧菌总数和葡萄球菌总数进行了检测,并对葡萄球菌的菌群组成进行了分析。结果表明,两个兔舍内需氧菌含量分别为1.73~85.8×103CFU/m3、2.71~9.66×103CFU/m3空气,葡萄球菌含量分别为0.94~7.84×103CFU/m3、1.02~6.54×103CFU/m3空气。兔舍空气中葡萄球菌主要包括金黄色葡萄球菌、腐生葡萄球菌、表皮葡萄球菌、科氏葡萄球菌、头状葡萄球菌和马胃葡萄球菌,其中金黄色葡萄球菌的含量占葡萄球菌总数的26.3%~29.6%,其次是腐生葡萄球菌和表皮葡萄球菌。另外,还对需氧菌和金黄色葡萄球菌在A ndersen-6级收集器不同层级上的分布情况进行了统计分析,结果表明,约有56.4%的需氧菌和49%金黄色葡萄球菌分布在3~6层上,空气动力学直径(A erody-nam ic d iam eter,D ae)在6~0.2μm,它们能进入人、畜的气管、支气管,甚至细支气管,对饲养员和动物的呼吸道构成严重危害。 相似文献
72.
AIM:To investigate the effect of cyclophosphamide(CTX) on proliferation and apoptosis of mesangial cells(GMC) of rat in vitro. METHODS:GMC proliferat ion was detected by MTT method,GMC apoptosis was examined by inverted microscopy for phase-contract and fluoroscopy and flow cytometry analysis.The levels of Fas and Bcl-2 were also detected by immunohistology. RESULTS:The proliferation of GMC were inhibited by CTX, methylprednisolone(MP), low molecular weight heparin(LMWH). Apoptosis of GMC was induced by CTX, the apoptosis rate of GMC was 8.2%, and the Fas level was increased. CONCLUSION:CTX could inhibit proliferation and induce apoptosis of GMC possibly by enhancing the Fas level. 相似文献
73.
AIM: To investigate the effect of IH764-3 on the apoptosis of H2O2-stimulated hepatic stellate cells(HSCs) and the alteration of focal adhesion kinase (FAK). METHODS: The proliferation of HSCs was examined by direct cell count and the apoptosis was determined by Annexin-V/PI labeling, while the morphological change was observed by light microscopy and transmission electron microscopy. In addition, FAK mRNA was detected by RT-PCR. RESULTS: H2O2 promoted the proliferation of HSCs. IH764-3 induced the apoptosis of HSCs in a dose-dependent manner. The HSC apoptotic rates of different groups were 6. 35%,9. 28%,15. 10%,19. 69%,respectively, after treated with different concentrations of IH764-3 for 48 h while H2O2 group showed 2. 30%. In 30 mg/L group, the apoptosis rates were 6. 73%、10. 34%、15. 10% for the indicated time periods(12 h, 24 h, 48 h). In the presence of IH764-3, FAK mRNA decreased. The FAK mRNA reduction began at 2 h after adding IH764-3. CONCLUSION: IH764-3 induced the apoptosis of HSCs. Down-regulating the expression of FAK mRNA may be one of its mechanisms. 相似文献
74.
JIANG An-li KANG Lu-dong ZHAO Chun-hua BI Wen-xiang HU Xiao-yan KONG Feng XU Song-de 《园艺学报》2003,19(1):55-58
AIM: In order to evaluate the applicable value of LDL as a targeted vehicle for chemotherapeutic agents, we investigated and compared the inhibitory effects of LDL-ACM complex and free ACM on nude mice's subcutaneous implanted tumors derived from gastric cancer cell lines, SGC-7901 and NKM-45. METHODS: LDL-ACM complex was prepared and the tumor model of nude mice was established by subcutaneous implantation of SGC-7901 and NKM-45. Then, the groups of nude mice developed subcutaneous implanted tumors were received either LDL-ACM complex or free ACM. Subsequently, the tumor size, weight and leukemia cell counts were measured and the rates of tumor-inhibition and the survival were compared among the groups. RESULTS: The inhibitory effects of LDL-ACM complex on the tumors, especially on SGC-7901 implanted tumors were much more obvious than that of free ACM. It was also indicated that the action of LDL-ACM complex was mediated by LDL receptor. CONCLUSION: These results showed that LDL-ACM complex had significant inhibitory effects on the implanted tumors and the effect might be mediated by LDL receptor. 相似文献
75.
AIM: To study the effect of cocaine on caspase-3 in myocardiac cells of male rats in different age. METHODS: Three-week-old(n=16), six-week-old(n=16) and twelve-week-old (n=16) male Sprague-Dawley rats were all divided into control groups and experiment groups randomly, each group had eight animals, experiment groups were given cocaine hydrochloride (15 mg·kg-1 body weight) subcutaneously daily for four weeks. The ratio of heart weight to body weight (HW/BW, mg/g) were measured. DNA fragmentation of myocardia cells was determined by gel electrophoresis, and caspase-3 activity in myocardia cells was tested by flow cytometry (FCM). RESULTS: In three experiment groups, the DNA isolated from myocardial cells displayed clear ladder pattern. The HW/BW and the caspase-3 activity were increased significantly than those of control groups (P<0.01). CONCLUSIONS: Cocaine induced apoptosis in rat myocardial cells. The increase in caspase-3 activity may be the one of important pathways related to the induction of apoptosis. 相似文献
76.
额尔齐斯河流域生态系统格局及变化 总被引:8,自引:4,他引:8
本文首先对额尔齐斯河流域气候,地貌,水文等环境因子特征进行了评述,进而阐述了流域生态系统的特征和格局以及在自然和人为干扰作用下的变化。分析结果表明在自然干扰作用下流域生态系统的格局与流域水文情势变化相适应,处于准稳定变化状态,而在人为对流域水文情势扰动下,流域生态系统的格局间接地受到影响。从而产生新的格局,其作用的强度,持久性和生态系统的格局变化程度过错大于大于自然干扰作用下的变化。引额调水工程对额尔齐斯河流域河谷生态系统内部单元格局变化具有重要影响。 相似文献
77.
库布齐沙漠穿沙公路沙害综合防治技术 总被引:20,自引:4,他引:20
库布齐穿沙公路修建在库布齐沙漠高大的流动沙丘区 ,风沙流侵袭与沙丘整体前移埋压公路是主要沙害形式。现有防沙措施是沙障保护下的人工植被及天然植被相结合的综合防护体系 ,固沙及阻沙效益明显。目前要解决的是尽快形成公路两侧稳定的植被群落 ,以保证公路的长期畅通 相似文献
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