论七体的形成和演进

七体是赋的分支 ,具有与其它赋类相区别的特点 ,其基本体制由西汉枚乘《七发》确立。七体在其流变过程中 ,招隐取代问疾成为题材上的突破 ,仕隐之辩为文人表述个人情性、价值取舍及德政标准提供了合适的途径。结构形式和语言运用上的创新也显示了作者们不囿于前人的努力     

鱼类对饲料中糖的利用研究进展

糖类是鱼类饲料中廉价的能量源,然而,与陆生动物相比,鱼类对饲料中糖的利用能力较低。本文综述了这方面的最新研究进展,包括鱼类对饲料糖的利用能力与鱼饲料中糖的适宜含量、影响因素及鱼类的糖代谢机制。     

广西10个地方优质品种鸡γ-干扰素基因的克隆与序列分析

根据基因库中鸡γ-干扰素的基因序列设计了1对特异性引物，应用反转录-聚合酶链式反应（RT-PCR）技术，从ConA诱导培养的广西10个地方优质品种鸡外周血淋巴细胞RNA中扩增了γ-干扰素基因，结果，均得到了大小为520 bp的特异性片段。将扩增产物纯化并克隆到pMD18-T载体上，获得重组质粒，经PCR和EcoRⅠ＋SalⅠ双酶切鉴定后测序。序列分析结果表明，广西10个地方优质品种鸡γ-干扰席基因均编码145个氨基酸的成熟蛋白，分子质量约为16．8ku，与哺乳动物和其他品种鸡的核苷酸序列同源性分别为38．8％～39．0％和99．2％～100％，氨基酸序列同源性分别为23．69／6～24．8％和97．6％～99．4％。     

水产饲料环保特性评价体系的初步构建

利用筛绢吊篓、烧杯、水浴锅等,于实验室内模拟水产养殖生产条件,分别测定了20℃和30℃水温下豆粕、鱼粉入水后的干物质溶失率、粗蛋白溶失量以及水质化学耗氧量、氨氮含量,对这两种蛋白饲料原料的环保特性进行了比较和评估,进而以设备条件、测定方法、测定指标等为主要内容,初步构建了水产饲料环保特性的评价体系。     

The Effect of Gender on Slaughtering Performance and Meat Quality in Sunite Sheep

The purpose of this experiment was to study the effect of gender on the slaughter performance and meat quality of Sunite sheep.Twelve 6-month-old Sunite rams and ewes at the same body condition were selected from the grazing herds of the Sunite Zuoqi Improvement Station Breeding Farm to determine their slaughter performance and meat quality.The slaughter test results showed that the bone quality and bone-meat ratio of the rams were significantly higher than that of the ewes (P<0.05),and the net meat quality and net meat rate of the ewes were significantly higher than that of the rams (P<0.05).The results of meat quality traits showed that the drip loss of ewes were significantly lower than that of rams (P<0.05),and the other indicators were not significantly different (P>0.05).The results of conventional nutrient composition of muscles showed that the intramuscular moisture content of the rams were significantly higher than that of the ewes (P<0.05),and the intramuscular fat content of the ewes were significantly higher than that of the rams (P<0.05),while the other indicators were not significantly different (P>0.05).The results of amino acid determination showed that the essential amino acid ratio in muscle of sunite ewes were significantly higher than that of rams (P<0.05),but there was no significant difference between the rams and ewes that formed the precursor amino acid content of meat flavor (P>0.05).Fatty acid measurement results showed that the saturated fatty acid ratio in the subcutaneous fat of rams was significantly lower than that of ewes (P<0.05).There was no significant difference in the ratio of saturated fatty acids in muscle fat and tail fat (P>0.05);The ratio of monounsaturated fatty acids in ewes tail fat was significantly higher than that of rams (P<0.05).The ratio of monounsaturated fatty acids in subcutaneous fat and intermuscular fat was not significantly different between rams and ewes (P>0.05).The ratio of polyunsaturated fatty acids in intermuscular fat of rams was significantly higher than that of ewes (P<0.05),and there was no significant difference in the content of polyunsaturated fatty acids in subcutaneous fat and tail fat (P>0.05).In summary,it showed that gender had a certain influence on the slaughter performance and meat quality of 6-month-old Sunite sheep.     

我国牧区畜牧业经营形式的历史沿革、分析及改革思路

本文通过阐述我国牧区畜牧业经营形式的历史演变及特点,推出了牧区畜牧业经营形式的两种模式,进一步结合经济学分析和现实问题,提出了牧区畜牧业经营形式改革的可行性和思路。     

不同蛋白质水平条件下氨基酸平衡日粮对生长猪氮、磷及能量代谢的影响

选用平均体重为(40±2)kg杜长大三元杂交去势公猪3头,按3×3的拉丁方进行试验设计,试验分3期,每期为7 d,采用全收粪法,研究不同蛋白质(CP)水平的氨基酸平衡日粮CP水平分别为14%、16%和18%)对氮、磷及能量代谢的影响。结果表明:不同蛋白质水平日粮处理间的氮表观消化率差异不显(著P>0.05),氮的沉积率以14%CP和16%CP组显著高于18%CP组(P<0.05),而14%CP和16%CP组之间无显著差异(P>0.05)。粪氮排出量14%CP日粮组显著低于16%CP和18%CP日粮组(P<0.05),16%CP日粮组和18%CP日粮组间无显著差异(P>0.05)。随着日粮蛋白质水平下降,生长猪的氮排出量、粪氮和尿氮均减少,总排泄氮中尿氮的比例较大,而粪氮则较少。不同蛋白质水平日粮对磷和能量的消化利用无显著影响(P>0.05)。     

牛传染性鼻气管炎病毒gD基因的克隆与表达

构建pET32a重组表达载体,转化到BL21(DE3),诱导表达牛传染性鼻气管炎病毒重组gD蛋白。用已发表的IBRV的gD基因序列设计一对特异性引物,通过PCR方法扩增一条766bp的目的基因gD,将gD基因克隆到原核表达载体pet32a,得到重组表达载体pet32-gD,转化到BL21(DE)中,通过IPTG诱导获得融合蛋白。重组表达蛋白经纯化后,免疫印迹分析。结论证明表达的重组蛋白具有良好的免疫原性。     

番茄资源材料的品质分析

观测分析了12份引进番茄新材料的主要品质指标。结果表明,材料间可溶性固形物含量、总酸度、番茄红素含量、VC含量差异明显,T31果实的番茄红素含量达到加工番茄标准;材料间外观品质在果面、果脐、生长习性上差异较小。综合表现较好的是PI120256、PI174263和T31,最差的是SnowWhite。GoldenRoma、VitaGold、HighCaroteneTM928、Sapho、HealthKick的利用价值有待进一步研究。     

0.1%氯吡脲可溶性液剂对西瓜产量和品质的影响

0.1%氯吡脲可溶性液剂50 mg/kg、45 mg/kg、40 mg/kg(毫克/公斤)(有效含量)涂抹西瓜雌花果柄可显著提高西瓜座果率,从而提高西瓜产量,同时在一定程度上可增加西瓜的含糖量和维生素C的含量.