Serologic correlation of suspected Leptospira interrogans serovar pomona-induced uveitis in a group of horses

After the observation of 2 horses with uveitis on a horse farm in the Minnesota River valley, 100 horses from this geographic area were given ophthalmologic examinations and were evaluated serologically for leptospirosis. A statistically significant (P less than 0.001) association was observed between the finding of antibodies against Leptospira interrogans serovar pomona and uveitis.     

Field release of a non-toxigenic Aspergillus flavus L strain in central Benin

Journal of Plant Diseases and Protection - Non-toxigenic strains of Aspergillus flavus (Link) can be used as biological control agents to reduce the contamination of grains such as maize with...     

Malignant lymphoma with cardiac and bone involvement in a dog

Malignant lymphoma involving bone and heart was diagnosed in a 4-year-old Whippet, using radiography, histologic examination, nuclear scintigraphy of the skeleton and heart, and cardiac ultrasonography.     

Use of a quantitative TaqMan-PCR for the fast quantification of mycobacteria in broth culture, eukaryotic cell culture and tissue

The quantification of slow-growing mycobacteria such as Mycobacterium tuberculosis or M. bovis from in vitro and in vivo samples is complicated by their long generation time, their ability to form aggregates, and their capacity to persist in a state of dormancy. We compared different methods for the establishment of growth curves for broth cultures of M. bovis bacille Calmette-Guérin (BCG). A quantitative TaqMan-PCR yielded results comparable with those obtained by protein quantification and measurement of the ATP content of the cultures. The quantitative TaqMan-PCR furthermore turned out to be particularly suitable for the measurement of multiplication of BCG within eukaryotic cells. Furthermore, it is a fast method allowing an estimation of the mycobacterial load in tissue long before colony counts can be obtained.