鸡大肠杆菌性脑病

作者发现大肠杆菌侵入鸡脑部引起昏睡、神经症状和下痢的脑病,且该菌在脑的分离成功率最高,命名为鸡大肠杆菌性脑病。发现血清型 O_(22)、O_(53)、O_(86):B_7等大肠杆菌能突破鸡的血脑屏障进入脑部引起如上脑病。本病既可在鸡的某些呼吸道病如滑膜霉形体病、败血霉形体病、传染性鼻炎、传染性喉气管炎的基础上继发或混合感染,又可独立发生。     

鸡与鸭饲料氨基酸消化率测定值比较

本研究应用排空强饲法及石岐杂公鸡、樱桃谷公鸭分别测定定了5种饲料氨基酸表观消化率和真消化率。结果表明：除玉米氨基酸表观消化率外,其余饲料的大多数氨基酸消化率在鸡和鸭之间都存在着明显差异;在32小时收粪时间里,鸭的内源氨基酸排出量明显比鸡多;内源氨基酸对鸡和鸭的饲料氨基酸真消化率都有影响,影响大小随蛋白质含量高低而异,一般饲料蛋白质含量越低的饲料两者相差越大。而内源氨基酸排出量对鸭饲料,尤其对低蛋白含量饲料的氨基酸真消化率的影响比鸡更明显。     

克伦特罗缓释药囊的制备,体外释药及对育肥猪生产性能的影响

选用可生物降的白氨酸，谷氨酸甲酯，谷氨酸三元聚物制成克伦特罗缓释药囊。体外释药研究所表明，药囊能保持长期缓慢恒速释药，释药速率随取合物中谷氨酸含量增多而增大，其中ＰＬＭＧＧ－２５的克伦特罗药囊释药时间为８０ｄ、稳态后每天释药量平均为１．２３ｍｇ／ｄ。     

牛暂时热研究进展

牛暂时热是一种发生于热带与温带地区牛的急性、免疫病理性病毒病，包括蚊、蠓在内的多种昆虫参与了该病的传播。本文就BEFV的病原学、流行病学、传播、防制、研究进展及研究趋势做了综述。     

Dietary amylose and amylopectin ratio and resistant starch content affects plasma glucose,lactic acid,hormone levels and protein synthesis in splanchnic tissues

Experiments were conducted to determine the effects of feeding different starch sources on piglets. Four diets were formulated with maize, brown rice, sticky rice and Hi‐Maize 1043 as starch sources, with resistant starch (RS) contents of 2.3%, 0.9%, 0.0%, 20.6%, and amylose and amylopectin ratio of 0.23%, 0.21%, 0.18%, 0.06% respectively. Fifty‐six pigs weaned at 28 days of age were randomly assigned to one of the four diets. In Exp. 1, six piglets in each group were fitted with an indwelling jugular catheter. After 25 days of feeding trial, venous blood samples were obtained at time zero and every 1 h for 4 h. In Exp. 2, the remaining piglets were used to determine the effects of different starch sources on the fractional synthesis rate (FSR). The results indicated that feeding the Hi‐Maize 1043 diet decreased (p < 0.05) plasma contents of glucose, insulin, lactic acid and T₃, while sticky rice increased plasma contents of glucose and insulin. The insulin contents in piglets fed the sticky rice diet was 69.2 μIU/ml at 1 h post‐feeding which was highest among the starch diets. The FSR in the pancreas, spleen, duodenum, jejunum, ileum and colon in the corn group were much higher (p < 0.05) than that in the sticky rice group. These results suggest that RS is potentially beneficial for improving insulin sensitivity in young pigs and that the ratio of amylose and amylopectin have significantly effects on the FSR in splanchnic tissues in weaned piglets. Another finding of this study indicated maize with a ratio of amylose and amylopectin of 0.23 has the best starch sources for pig production.     

新城疫病毒实时荧光RT-PCR检测方法的建立与应用

为满足新城疫病毒高通量快速检测的需要,针对新城疫病毒M基因序列,通过基因比对分析保守区域,经同源性分析后,设计合成多条引物和探针并对其筛选,建立了一种能够快速检测新城疫病毒的实时荧光RTPCR检测方法,并对该方法的特异性、灵敏性进行了评估。结果显示,该方法检测耗时短、特异性好,检测下限达10~(-4) ng/μL。利用该方法,对480份临床采集的各类家禽咽拭子样品进行检测,共检测出25份阳性,与常规RT-PCR检测方法结果一致,κ值为1(P 0.001)。结果表明,该方法快速、准确,可用于新城疫病毒的快速检测。     

我国居民肉类消费情况调查

为更好研究落实2020年中央1号文件作出的决策部署,对居民肉类消费情况进行了调查。本次调查对全国30个省(区、市)各年龄层次、收入水平的1 060名居民,进行了猪肉、牛肉、羊肉、禽肉、水产品等肉类消费情况和习惯摸底。结果显示:2019年我国居民日常猪肉消费量有所降低,但猪肉仍是主要肉类消费产品;居民喜食热鲜猪肉的消费习惯已发生变化,对冷鲜猪肉接受度较高,对冷冻猪肉接受度低;卫生安全和价格是影响居民对猪肉消费决策的主要因素。可考虑从创新检疫工作模式,重点扶持"养殖-屠宰-销售"一体化企业,强化冷冻肉技术研发和推广,利用新媒体进行舆论引导等4个方面,进一步提高居民对冷鲜、冷冻肉品的认可度,为未来推动从"调猪"向"调肉"转变提供良好的市场环境。     

Evaluation of immunogenicity and protective efficacy of Actinobacillus pleuropneumoniae HB04C(-) mutant lacking a drug resistance marker in the pigs

Previously, we reported the construction and characterization of a genetically defined Actinobacillus pleuropneumoniae (A. pleuropneumoniae) apxIIC gene mutant, HB04C(-), which conferred protection to mice against infection with A. pleuropneumoniae. In this study, we further evaluated HB04C(-) for safety and its ability to elicit protective immunity in pigs. It was demonstrated that a dose of 2 x 10(8) CFU HB04C(-) was safe to the pigs via intranasal or intramuscular injection. Immunization with a dose of 2 x 10(8) HB04C(-) by both intranasal and intramuscular routine could yield equal protective efficacy and elicited significant protection against experiment challenge with homologous or heterologous serotypes of a virulent A. pleuropneumonia. Taken together, HB04C(-) might serve as a promising vaccine candidate against infection with A. pleuropneumoniae.     

企业如何实现零呆帐销售?

所谓零呆帐销售是一种与时俱进确保企业盈利的营销策略,也是一种追求“机会最大化,风险最小化”的营销战术,更是一种讲究“销前预防、销中控制、销后催款”的企业文化,这是摆脱“冒险销售”这个紧箍咒的最好办法,能够帮助企业百分之百收回应收帐款,保证利润百分之百落地生根变呆帐为零。