高校心理健康教育网络课程模式的构建与实例分析

计算机多媒体技术和网络技术日新月异的发展,教育信息化的推进,为努力实现高校心理健康教育课程教学内容、教学手段和教学方法的全面创新,本文以《心理健康教育》网络课程为例,尝试剖析其在模式构建、教学设计、交互安排、教学应用等方面的特征,以期为开辟高校心理健康教育课程的新模式提供思路。     

Increasing selenium supply during the close-up dry period improves nutrient metabolism and attenuates inflammatory response after calving in dairy cows

The objective of this study was to investigate whether feeding selenium (Se)-replete cows a Se-yeast supplement in late pregnancy affects nutrient metabolism and inflammatory response during the periparturient period. Twenty cows were randomly assigned to two groups with 10 cows each. Cows in one group received Se-yeast at 0.3 mg Se/kg DM during the last 4 weeks before calving in addition to fed a TMR containing supplemented sodium selenite at 0.3 mg Se/kg DM (Se-yeast), while cows in another group were only fed a TMR containing supplemented sodium selenite at 0.3 mg Se/kg DM (Control). Blood samples were collected and analyzed for nonesterified fatty acids (NEFA), β-hydroxybutyrate (BHBA), glucose, insulin, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, serum amyloid A (SAA), haptoglobin (Hp), and albumin. In control cows, plasma NEFA, IL-1β, IL-6, SAA, and Hp levels increased after calving, but glucose, insulin, and albumin levels decreased after parturition. Se-yeast supplemental cows had lower postpartum concentrations of NEFA, TNF-α, IL-1β, IL-6, SAA, and Hp, and higher postpartum levels of glucose, insulin, and albumin compared with control cows. The results indicate that feeding Se-replete cows a Se-yeast supplement in late pregnancy improves nutrient metabolism and attenuates the inflammatory response after calving.     

中喙丽金龟成虫对不同植物种类的危害程度

调查了近年来在上海局部区域严重发生的农林害虫——中喙丽金龟成虫对不同植物种类的危害程度。共计调查243个植物种类,其中109种植物受害,占调查植物种类数量的44.86%。109种受害植物隶属于39个科,以蔷薇科、豆科植物种类最多。     

峨眉山野生茶树资源农艺性状多样性分析

为挖掘峨眉山野生茶树和群体种中的优良资源,利用因子分析、聚类分析和感官审评等方法,对峨眉山40份野生茶树种质资源农艺性状进行遗传多样性分析和资源评价。结果表明,峨眉山茶树种质资源丰富,且多以灌木型为主;18个芽叶形态性状的变异系数为7.70%~60.99%,变异系数最大的性状是芽叶色泽(60.99%)。因子分析表明,18个芽叶形态性状综合为6个公因子,前6个公因子的特征值大于1且累计贡献率达80.74%,主要代表性指标为叶片大小、叶片锯齿和叶面特征等。聚类分析将研究材料分为5个大的类群,显示了复杂的亲缘关系和丰富的遗传多样性。适制性研究表明,初步筛选的11份种质资源制绿茶适制性较好,感官审评总分在92分以上,大体表现为香气鲜香带花香、清香高长带花香或栗香浓郁带花香,滋味鲜爽、鲜甜或鲜浓醇。     

Molecular cloning and sequence analysis of an LFY homologous gene from Juglans regia L.

The existence of a long juvenile phase is found seriously to affect the early-stage economic benefits of later mature walnuts (Juglans regia L.). Studies on LFY, a key gene controlling floral transition and flower differentiation, may be helpful in solving the problem. This study reports the identification and characterization of a JrLFY gene from Juglans regia L., a homolog of FLORICAULA/LFY. The gene was isolated from flower buds of precocious walnut cultivar Zhonglin No. 5 by RT-PCR and RACE. The cDNA sequence of JrLFY (GenBank accession no. GU194836) was 1496 bp and contained an ORF of 1158 bp. Its corresponding genomic sequence (GenBank accession no. HQ019159) showed that the JrLFY contained three exons and two introns. The predicted amino acid sequence of the gene consisted of 385 amino acids and had a conserved region in the C-terminal when being aligned with sequences of other LFY homologs. Phylogenetic analysis showed that the LFY protein of walnut was close to those of hickory and chestnut. These studies will lay a foundation for understanding the mechanism of early fruiting and preparation for transfer of the JrLFY as a transgene to later mature walnuts.     

省工棉新品种选育简报

介绍了省工棉的背景、特点、栽培技术及所选育的省工棉品种的特征特性,井提出了省工棉的发展方向。     

超氧化物歧化酶模拟物对氧化应激IPEC-J2细胞抗氧化性能的影响

试验旨在探究在H₂O₂诱导的氧化应激状态下，超氧化物歧化酶模拟物（SODm）对仔猪空肠上皮细胞系（IPEC-J2）的保护作用。利用MTT法筛选出构建氧化应激模型H₂O₂的适宜浓度；将IPEC-J2细胞分别用0、0.05、0.5、5、50、500、2 000 U/mL SODm进行培养，利用MTT法分别在2、4、8、12 h时测定各组细胞存活率，筛选出SODm作用的适宜浓度和时间；根据构建的氧化应激模型和SODm适宜浓度和时间，将IPEC-J2细胞随机分为空白组、模型组、SODm处理组（SODm_0.5、SODm₅、SODm₅₀）和超氧化物歧化酶（SOD）处理组（SOD_0.5、SOD₅、SOD₅₀），分别测定各组细胞存活率、活性氧（ROS）含量及细胞内SOD、谷胱甘肽过氧化物酶（GSH-Px）活性及丙二醛（MDA）含量和总抗氧化能力（T-AOC）。结果表明：①H₂O₂构建细胞氧化应激模型的适宜浓度是1.0 mmol/mL。②当不同浓度SODm分别作用4、8、12 h时，0.5~50 U/mL SODm组细胞存活率显著高于空白组（P<0.05）；且8 h时存活率最高，在12 h时处于下降趋势。③除空白组外，SODm₅和SOD₅预处理的IPEC-J2存活率显著高于其他组（P<0.05）；且SODm和SOD组中细胞ROS含量显著低于模型组（P<0.05）；模型组与空白组相比，均显著降低了SOD、GSH-Px活性和T-AOC水平，提高了MDA含量（P<0.05）；与模型组相比，所有SODm和SOD处理组均显著提高了SOD、GSH-Px活性和T-AOC水平，降低了MDA含量（P<0.05），同时SODm₅₀组T-AOC水平显著低于SOD₅₀组（P<0.05）。综上，SODm对H₂O₂诱导氧化损伤状态下IPEC-J2具有保护作用。     

茶和天下语境下历史典故的重温与传播——以“以茶代酒”为例

中华茶文化历史源远流长,文化内容博大精深,是中国文化乃至世界文化重要的组成部分。然而,历史茶文化尤其是历史典故,大多需要重新解读,只有将历史与现实结合起来,有针对性且有说服力地赋予新的时代内涵,才能扩大其在世界范围的价值认同。基于此,本文以“以茶代酒”为例,将其置于茶和天下的语境下,在重温这一历史典故的基础上,从增加中华茶文化魅力,促进历史茶文化的传播的角度出发,分析探讨了以茶代酒的当代价值或中华茶文化核心价值的当代体现,并根据全球化与茶文化传播的需要,从增强自信性、构建“同型性”、抢占先机性、设计备选性等方面,提出了如何通过以茶代酒,推动中华茶文化核心价值实现的相关策略。